2020
DOI: 10.1038/s41592-020-01011-4
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Screening for functional circular RNAs using the CRISPR–Cas13 system

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Cited by 232 publications
(161 citation statements)
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“…The knockdown efficiency of all available Cas13 family proteins (LwaCas13a, PguCas13b, PspCas13b, RanCas13b, AdmCas13d, EsCas13d, or RfxCas13d) were compared for circPOLR2A and circRTN4 in vitro. 35 Most of them could knockdown the circRNAs with no detectable effects on their linear counterparts, but RfxCas13d exhibited the highest knockdown efficiency. RfxCas13d was used to target many other circRNAs, and results showed specific and robust knockdown of all circRNAs.…”
Section: Strategies To Target Circrnasmentioning
confidence: 99%
See 1 more Smart Citation
“…The knockdown efficiency of all available Cas13 family proteins (LwaCas13a, PguCas13b, PspCas13b, RanCas13b, AdmCas13d, EsCas13d, or RfxCas13d) were compared for circPOLR2A and circRTN4 in vitro. 35 Most of them could knockdown the circRNAs with no detectable effects on their linear counterparts, but RfxCas13d exhibited the highest knockdown efficiency. RfxCas13d was used to target many other circRNAs, and results showed specific and robust knockdown of all circRNAs.…”
Section: Strategies To Target Circrnasmentioning
confidence: 99%
“… 33 , 34 Furthermore, CRISPR technology and Cas13 systems in particular have demonstrated great potential in knocking down circRNAs in a specific and robust manner. 35 , 36 …”
Section: Introductionmentioning
confidence: 99%
“…METLIN MS2, is chemical standards spectral DB that is well annotated and structurally diverse database consisting of over 850,000 chemical standards with MS/MS data generated in both positive and negative ionization modes at multiple collision energies (CEs), collectively containing over 4,000,000 curated HR MS/MS data that covers almost 1% of PubChem’s 93 million compounds (Xue et al 2020 ).…”
Section: Databasesmentioning
confidence: 99%
“…There is no standard nomenclature of circRNAs, although the study of circRNAs has exploded in the past few years, which is not convenient for academic exchanges (155). Many individual circRNA functions remain unknown due to the lack of adequate methods for distinguishing circRNAs from cognate mRNAs with overlapping exons (156). RNA sequencing (RNA-seq) data are used to detect genome-wide circRNA expression by numerous algorithms, but there is no clear standard way to evaluate the accuracy of these algorithms and little overlap in their predictions (157).…”
Section: Conclusion and Future Perspectivesmentioning
confidence: 99%