Screening for library-assisted identification and fully validated quantification of 22 beta-blockers in blood plasma by liquid chromatography–mass spectrometry with atmospheric pressure chemical ionization

Maurer, Hans H.; Tenberken, Oliver; Kratzsch, Carsten; Weber, Achim; Peters, Frank T.

doi:10.1016/s0021-9673(04)01308-1

Cited by 80 publications

(16 citation statements)

References 28 publications

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“…Hence, for TDM studies, a simple and fast procedure is preferred. Liquid-liquid extraction, 11 solid-phase extraction, 12 and protein precipitation (PPT) 13 are the main techniques used to pretreat the samples prior to monitor levels of drugs in plasma, serum, and urine samples. These methods suffer from high consumption of organic solvents and relatively long processing time.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Ultrasound Assisted and Dispersive Liquid-Liquid Microextractions Combined with HPLC-UV Method for Determination of Bosentan in Human Plasma and Urine

Sajedi-Amin

Asadpour‐Zeynali

Khoubnasabjafari

et al. 2016

J. Braz. Chem. Soc.

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A feasible, rapid, sensitive and reliable dispersive liquid-liquid microextraction (DLLME) and ultrasonic assisted liquid-liquid microextraction (USAEME) combined with high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for analysis of bosentan in human plasma and urine samples. All validation tests were reported according to FDA guidelines. Since chromatographic methods are precise, accurate and provide excellent separation are increasingly being used in determination of trace amount of drugs in biological goals. So the optimized experimental extraction factors from our previous work were used to evaluate HPLC-UV method for decreasing the limit of quantifications (LOQs) to cover the therapeutic range. Simple centrifugation of urine samples was sufficient prior to microextraction procedure, while acetonitrile protein precipitation provides sample clean up for plasma samples. The suggested method was linear (correlation coefficient R 2 > 0.998) over a range of 0.1-10 μg mL -1 which covers the therapeutic range. Proposed methods are practical for quantitative analysis of bosentan in real samples, and could be used in routine clinical analyses.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of Ultrasound Assisted and Dispersive Liquid-Liquid Microextractions Combined with HPLC-UV Method for Determination of Bosentan in Human Plasma and Urine

Sajedi-Amin

Asadpour‐Zeynali

Khoubnasabjafari

et al. 2016

J. Braz. Chem. Soc.

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“…A comparison of the results obtained by our method with others is clearly shown in Table 1. As can be seen, the detection limits of AT and ME, obtained by our method, were lower than that of HPLC coupling with a capacitively coupled contactless conductivity detector (HPLC-C4D), 14 HPLC-ECL 17 and CE-UV, 21 and were comparable to HPLC-MS, 16 but were higher than that of HPLC-MS/MS. 15 …”

Section: Reproducibility Detection Limits and Linearitymentioning

confidence: 69%

“…6,7 However, the sensitivity of these methods was limited. The most widely used were chromatographic techniques, such as gas chromatography (GC), 8,9 high-performance liquid chromatography (HPLC) [10][11][12][13][14][15][16][17] and urtra-performance liquid chromatography (UPLC). 18 Among them, expensive columns and costly apparatus were needed, and the analysis time was relatively long.…”

Section: Introductionmentioning

confidence: 99%

Determination of Atenolol and Metoprolol by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection

et al. 2007

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Atenolol (AT) and metoprolol (ME) are antihypertensive drugs that belong to the family of selective β(1)-adrenergic receptor antagonists (β-blockers), which are widely used in the treatment of hypertension, angina pectoris, cardiac arrhythmias and myocardial infarction. 1 Due to their sedative effect, they are also used as doping agents in sports. An overdose of β-blockers may lead to bradycardia, hypotension, aggravation of cardiac failure, bronchospasm, hypoglycemia, and fatigue.2 Therefore, it is necessary to develop a sensitive and simple analytical method to determine β-blockers in many fields, including doping control, forensic analysis, toxicology and pharmacokinetic study.As a consequence of the widespread use of AT and ME, several analytical techniques have been developed for their determination in recent years, including spectrophotometry [3][4][5] and differential pulse voltammetry. 6,7 However, the sensitivity of these methods was limited. The most widely used were chromatographic techniques, such as gas chromatography (GC), 8,9 high-performance liquid chromatography (HPLC) [10][11][12][13][14][15][16][17] and urtra-performance liquid chromatography (UPLC). 18 Among them, expensive columns and costly apparatus were needed, and the analysis time was relatively long.The capillary electrophoresis (CE) technique has been developed for over 20 years, and has become a mature and wellestablished analytical tool. CE is characterized as a powerful separation technique because of its high efficiency, relatively short analysis time, and low sample and reagent consumption. As an alternative and complementary technique to HPLC for routine analysis, CE-UV has been used for AT and ME analysis. 19-22Because of the small sample volume being assayed, this method has encountered the problem of poor sensitivity, which has limited its application in practice. He et al. 23 developed an on-line ion-exchange preconcentration method for improving the detection sensitivity of CE-UV. The proposed method enhanced the detection sensitivity of CE-UV by about 5000 times for metoprolol compared with the normal electrokinetic injection.However, the preparation of a preconcentration column was complex for a general laboratory. Thus, it is very important to develop a simple and sensitive method to detect AT and ME.Electrochemiluminescence (also called electrogenerated chemiluminescence) is a means of converting electrical energy into radiative energy. 24 It has been proved to be a powerful analytical tool that combines the simplicity of electrochemistry and the inherent sensitivity and the wide linear range of the chemiluminescence method. 25Among the ECL systems, tris(2,2′-bipyridyl)ruthenium(II) Ru(bpy)3 2+ -based ECL is the most valuable in fundamental studies and applications because of its stability and capability of undergoing ECL at room temperature in aqueous solutions and its regenerating during the ECL process. 26Since the first report of Ru(bpy)3 2+ ECL in 1972, 27 Ru(bpy)3 2+ ECL as a sensitive detection method has been...

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“…The sensitivity of the proposed method was approximately 600, 60 and 14 times higher than that of HPLC with amperometric detection [17], ELISA [18] and HPLC with atmospheric pressure chemical ionization mass spectrometry [19]. Although the sensitivity of the proposed method was comparable with that of HPLC with tandem mass spectrometry [20], the proposed method does not require expensive and elaborate instruments.…”

Section: Calibration Curve Detection Limit and Repeatabilitymentioning

confidence: 93%

Labeling of alprenolol with fluorescent aryl iodide as a reagent based on Mizoroki–Heck coupling reaction

Kishikawa

Ohkuma

Wada

et al. 2011

Journal of Chromatography A

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A novel fluorescent labeling method for alprenolol was developed based on Mizoroki-Heck coupling reaction. We designed and synthesized fluorescent aryl iodide, 4-(4,5-diphenyl-1H-imidazol-2-yl)iodobenzene (DIBI) as a labeling reagent.DIBI has a lophine skeleton carrying an iodide atom acting as fluorophore and reactive center, respectively. In order to evaluate the usefulness of DIBI, a highperformance liquid chromatography (HPLC) with fluorescence detection method was developed for the determination of alprenolol as a model compound of terminal double bond. The fluorescent labeling of alprenolol with DIBI was achieved in the presence of palladium acetate as a catalyst, and the labeled alprenolol was detected fluorometrically. In addition, it was found that the fluorescence of DIBI derivative increased and red shifted when compared that of DIBI. Furthermore, the proposed method could be applied to determine the alprenolol concentration in rat plasma after administration of alprenolol without interferences from biological components. The detection limit (S/N=3) for alprenolol in rat plasma was 0.74 ng/mL (30 fmol on column).

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Screening for library-assisted identification and fully validated quantification of 22 beta-blockers in blood plasma by liquid chromatography–mass spectrometry with atmospheric pressure chemical ionization

Cited by 80 publications

References 28 publications

Development and Validation of Ultrasound Assisted and Dispersive Liquid-Liquid Microextractions Combined with HPLC-UV Method for Determination of Bosentan in Human Plasma and Urine

Development and Validation of Ultrasound Assisted and Dispersive Liquid-Liquid Microextractions Combined with HPLC-UV Method for Determination of Bosentan in Human Plasma and Urine

Determination of Atenolol and Metoprolol by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection

Labeling of alprenolol with fluorescent aryl iodide as a reagent based on Mizoroki–Heck coupling reaction

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