Screening for Resistance Against Pulse Beetle, Callosobruchus analis (F.) in Greengram (Vigna radiata (L.) Wilczek) Accessions

Abstract

The susceptibility of eighty five green gram accessions were evaluated against pulse beetle, Callosobruchus analis (F.) under laboratory conditions. Accessions which exhibited lesser susceptibility index during the preliminary free choice tests were further subjected to 'force choice' test for confirmation of bruchid resistance. No green gram accession was found immune to bruchid infestation and showed significant difference in terms of oviposition, developmental period, adult emergence and seed weight loss. Two accessions viz, Km-12-5 and P-S-16 revealed lesser per cent adult emergence of 12.22 and 14.29, respectively. Prolonged developmental period (25.67 and 26.00 days, respectively) and lesser susceptibility index (0.042 and 0.044 respectively) in comparison to highly susceptible Ganga 8, which exhibited a survival of 71.67 %, developmental period of 20.67 days and susceptibility index of 0.09. Correlation between GI and other growth parameters of pulse beetle on different accessions indicated that GI had negative relationship with mean developmental period (-0.924) and significant positive relationship with adult emergence (0.931), weigh loss (0.952) and eggs laid (0.734). Hence, these accessions can be effectively used as promising donors for developing bruchid resistant varieties which would ensure food security by reducing postharvest losses under storage conditions.

Gas Chromatography-Mass Spectrometry (GC-MS) based Metabolomics of Promising Chickpea Genotypes against Callosobruchus chinensis (L.)

“…Whereas, highest GI (0.073) was observed in JG11 (0.073). These results are in agreement with the observations of Soumia et al (2017) who reported GI of C. analis ranging from 0.042-0.09 in green gram genotypes. The minimum per cent weight loss was recorded on ICCV07108 (4.22) followed by ICCV92944 (10.71), HC1 (11.23).…”

Host Plant Resistance and Analysis of Chemical Compounds Responsible for Bruchid Resistance in Greengram Vigna radiata (L.) Wilczek

“…The mean developmental period recorded was 27 days (GGISC 140), 26 days (GGISC 124, GGISC 150) and 23 days in the susceptible check VBN (Gg) 2. Soumia et al (2017) also reported longer developmental period in resistant lines when compared to susceptible lines in greengram. The weight of 10 pairs of bruchids were 45.00 mg in VBN (Gg) 2 and however sufficient number of bruchids was not emerged in the specified time (30 days) inorder to record weight of the bruchids.…”

Host Plant Resistance for Bruchids in Pre-breeding Lines of Greengram [Vigna radiata (L.) Wilczek]

“…sublobata) reached 100 and 12 per cent adult emergence, respectively on 30 th day of screening. Soumia et al (2017) reported that the reduction adult emergence is an indication of the presence of antinutritional factors in seed that results in the prolongation of developmental period of bruchid in greengram.…”

“…Samyuktha et al (2020) screened VBN (Gg) 2 for bruchid resistance by using no choice method of screening and also used weight loss per cent and susceptibility index for assessing the resistance level and concluded that VBN (Gg) 2 belongs to susceptible category. Sarkar and Bhattacharyya (2015) and Soumia et al (2017) reported that the susceptibility index was more than 0.05 and seed damage was more than 40 per cent in susceptible varieties of greengram.…”