Cotton screening was conducted at the Regional Agricultural Research Station (RARS) in Warangal, Telangana, India. The experimental material consisted of 52 cotton germplasms and 13Bt cotton hybrids with a check were tested against bacterial blight and tobacco streak virus diseases duringKharif-2016 at RARS,Warangal. Out of 52 cotton germplasms,17 entries viz.,HYPS-152,H-1250,RAH-4,KH-2244N,LH-900,SA-434,RAH-12,JK-354,GBHU-164,NA-340,BS-37,TCH-724, ICMF-23,ICMF-20,SA-1004,ARB-89001 and BB-2 were found resistant to bacterial blight disease and eighteen entries namely.,SA-53-1,RHH-101,KH-2244N,KH-134,TCH-1649,NH-557,IH-08,PH-1008,TCH-724,JK-205,MRK-38,LH-2170,AKH-2822,ARB-8901,G-COT-10,JK2764,CCH 1071 and CCH-11germplasms were noticed tobacco streak virus disease rating scale 0 (Immune). Out of 13 Bt cotton hybrids , two entries viz., Balhwan and Raja were moderately resistant to bacterial blight disease and eleven entries namely viz., Bhakti, Raja, Balhwan, Akka, First class,RCH-812,Ankur-3224,ACH-199,RCH-836,RCH-812 and ATM entries were showed disease rating scale 1(resistant) to tobacco streak virus disease.