Screening of multiple hormonal activities in surface water and sediment from the Pearl River system, South China, using effect‐directed in vitro bioassays

Zhao, Jian‐Liang; Ying, Guang‐Guo; Yang, Bin; Liu, Shan; Zhou, Lijun; Chen, Zhi Feng; Lai, Hua‐Jie

doi:10.1002/etc.625

Cited by 62 publications

(37 citation statements)

References 46 publications

(60 reference statements)

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“…The recombinant yeast was donated by J. P. Sumpter (Brunel University, Uxbridge, United Kingdom). The YES assay was conducted according to the method described by Zhao et al 26 In brief, a sample extract was diluted by twofold in series, then 10 mL sample of each concentration was transferred to the corresponding wells on a 96-well plate. The solvent was allowed to dry in a laminar ow cabinet, and then 200 mL yeast solution in growth medium was added to each well to obtain a yeast density of 4 Â 10 7 cells per mL and chlorophenol red-b-D-galactopyranoside concentration of 0.1 mg mL À1 in the well.…”

Section: Yeast Estrogen Screen (Yes) Assaymentioning

confidence: 99%

Simultaneous removal of inorganic and organic compounds in wastewater by freshwater green microalgae

Zhou

Ying

Liu

et al. 2014

Environ. Sci.: Processes Impacts

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143

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Batch experiments were carried out for 7 days to investigate the simultaneous removal of various organic and inorganic contaminants including total nitrogen (TN), total phosphorus (TP), metals, pharmaceuticals and personal care products (PPCPs), endocrine disrupting chemicals (EDCs), and estrogenic activity in wastewater by four freshwater green microalgae species, Chlamydomonas reinhardtii, Scenedesmus obliquus, Chlorella pyrenoidosa and Chlorella vulgaris. After treatment for 7 days, 76.7-92.3% of TN, and 67.5-82.2% of TP were removed by these four algae species. The removal of metals from wastewater by the four algae species varied among the metal species. These four algae species could remove most of the metals efficiently (>40% removal), but showed low efficiencies in removing Pb, Ni and Co. The four algae species were also found to be efficient in removing most of the selected organic compounds with >50% removal, and the estrogenic activity with removal efficiencies ranging from 46.2 to 81.1% from the wastewater. Therefore, algae could be harnessed to simultaneously remove various contaminants in wastewater. Environmental impactDomestic wastewater contains various inorganic and organic contaminants, which could pose risks to the environment and public health. To avoid these adverse effects, wastewater must be treated prior to its nal discharge into the receiving environment. Unfortunately, incomplete removals have oen been reported for these contaminants in wastewater by conventional wastewater treatment technologies. The use of microalgae in the treatment of wastewater has gained great interest over the past few years. However, previous studies on wastewater treatment by algae have been limited and most of them are focused on the removal of inorganic or organic pollutants in articial wastewaters. This study investigated the simultaneous removal capacity of inorganic (nitrogen, phosphorus and metals) and organic pollutants (PPCPs and EDCs) by four freshwater microalgae, and also evaluated the elimination of estrogenic activity in the wastewater by these algae species. The results demonstrated that simultaneous removal of various contaminants in wastewater was achieved, which showed potential application of these algae species in the wastewater treatment.

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Section: Yeast Estrogen Screen (Yes) Assaymentioning

confidence: 99%

Simultaneous removal of inorganic and organic compounds in wastewater by freshwater green microalgae

Zhou

Ying

Liu

et al. 2014

Environ. Sci.: Processes Impacts

Self Cite

143

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“…The YAS assay was performed according to the method developed previously by Sohoni and Sumpter (1998) with some modification, which was described in detail by Zhao et al (2011b). In brief, dihydrotestosterone (DHT) was used as the positive control with an initial concentration of 2 μM, and methanol was used as the blank control.…”

Section: Recombinant Yeast Screening Of Hormonal Activitiesmentioning

confidence: 99%

Hormonal effects of tetrabromobisphenol A using a combination of in vitro and in vivo assays

Huang

Ying

Liang

et al. 2013

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…The methods for YAES originated from the YES assay with some modifications, which were described by Zhao et al [39]. During the procedure, dilutions of extracted effluent samples each were treated with 10 ml E2 standard (1.36 mg/L), which is sufficient to cause submaximal estrogenic activity.…”

Section: Yeast Anti-estrogen Screen Assaymentioning

confidence: 99%

“…All media and strains for assays were prepared according to the original protocol for the YAS [40], and the assay procedure was modified from the procedure for the YES, which was described by Zhao et al [39]. In the YAS assay, dihydrotestosterone (DHT) standard reagent (final concentration: 7 Â 10 À8 M to 5 Â 10 À10 M) was used as the positive control and the methanol as the blank control.…”

Section: Yeast Androgen Screen Assaymentioning

confidence: 99%

“…For screening of anti-androgenic activity, the method originated from the YAS assay with some modifications was described by Zhao et al [39]. Briefly, all dilutions of extracted effluent samples were each treated with 10 ml DHT as standard reagents (43.6 mg/L; final concentration 2.18 mg/L), which is sufficient to cause submaximal androgenic activities.…”

Section: Yeast Androgen Screen Assaymentioning

confidence: 99%

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Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Fang

Ying

Zhao

et al. 2012

Enviro Toxic and Chemistry

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Wastewaters from various industries are a main source of the contaminants in aquatic environments. The authors evaluated the hormonal activities (estrogenic/anti‐estrogenic activities, androgenic/anti‐androgenic activities) and genotoxicity of various effluents from textile and dyeing plants, electronic and electroplate factories, pulp and paper mills, fine chemical factories, and municipal wastewater treatment plants in the Pearl River Delta region by using in vitro bioassays (yeast estrogen screen [YES]; yeast androgen screen [YAS]; and genotoxicity assay [umu/SOS]) combined with chemical analysis. The results demonstrated the presence of estrogenic, anti‐estrogenic, and anti‐androgenic activity in most industrial effluents, whereas no androgenic activities were detected in all of the effluents. The measured estrogenic activities expressed as estradiol equivalent concentrations (EEQs) ranged from below detection (3 of 26 samples) to 40.7 ng/L, with a mean of 7.33 ng/L in all effluents. A good linear relationship was found between the EEQs measured by YES bioassay and the EEQs calculated from chemical concentrations. These detected estrogenic compounds, such as 4‐nonylphenol and estrone, were responsible for the estrogenic activities in the effluents. The genotoxic effects expressed as benzo[a]pyrene equivalent concentrations (BaP EQs) varied between below detection and 88.2 µg/L, with a mean of 8.76 µg/L in all effluents. The target polycyclic aromatic hydrocarbons were minor contributors to the genotoxicity in the effluents, and some nontarget compounds in the effluents were responsible for the measured genotoxicity. In terms of estrogenic activities and genotoxicity, discharge of these effluents could pose high risks to aquatic organisms in the receiving environments. Environ. Toxicol. Chem. 2012;31:1273–1282. © 2012 SETAC

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Screening of multiple hormonal activities in surface water and sediment from the Pearl River system, South China, using effect‐directed in vitro bioassays

Cited by 62 publications

References 46 publications

Simultaneous removal of inorganic and organic compounds in wastewater by freshwater green microalgae

Simultaneous removal of inorganic and organic compounds in wastewater by freshwater green microalgae

Hormonal effects of tetrabromobisphenol A using a combination of in vitro and in vivo assays

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

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