Screening of plant extracts for anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Huang, Aiguo; Yi, Yanglei; Ling, Fei; Lu, Lin; Zhang, Qizhong; Wang, Gao‐Xue

doi:10.1007/s00436-013-3597-7

Cited by 32 publications

(22 citation statements)

References 20 publications

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“…Wang et al (2010a) found that the efficacy of steroidal saponins from Dioscorea zingiberensis was up to 90% and Wang et al (2011) found that osthol extract from Radix angelicae pubescentis had efficacy of 100%. On the other hand, Huang et al (2013) reported that ethyl acetate extract from Lysima chiachristinae presented 50% efficacy against D. intermedius.…”

Section: Discussionmentioning

confidence: 95%

Essential oils of Lippia sidoides and Mentha piperita against monogenean parasites and their influence on the hematology of Nile tilapia

et al. 2016

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This study evaluated the use of therapeutic baths containing essential oils of Lippia sidoides (pepper rosemary) and Mentha piperita (peppermint) on the hematological parameters of Nile tilapia parasitized by the monogeneans Cichlidogyrus tilapiae, Cichlidogyrus thurstonae, Cichlidogyrus halli, and Scutogyrus longicornis. A total of 320 juvenile fish were distributed into 16 tanks of capacity 100 L (20 fish per tank), divided into 4 treatments in quadruplicates: fish exposed to a bath of L. sidoides at 20 mg L −1 ; fish exposed to M. piperita at 40 mg L −1 ; fish exposed only to a water bath; and fish exposed to water + DMSO (dimethyl sulfoxide) bath. The fish were subjected to 3 baths for 10 min, at intervals of 24 h between treatments. After the third bath, parasitological and hematological analyses were performed. The parasite prevalence in fish treated with essential oils was seen to have decreased by 70%. The efficacy attained among fish treated with L. sidoides, in comparison with control water and water + DMSO, was 1.96% and 14.16%, respectively; and among fish treated with M. piperita, it was 33.33% and 41.63%, respectively. The total numbers of red blood cells (RBC) and thrombocytes were lower in fish treated with L. sidoides. Glucose concentration and neutrophil count were significantly higher in fish treated with L. sidoides. Because of the efficacy and positive hematological results, we suggest that baths of M. piperita at 40 mg L −1 should be used as anthelmintic action. Statement of relevance: Authors believe on the use of essential oils to treat ectoparasites of cultured fish and consequently no damages for hematological profile of Nile tilapia were found.

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Section: Discussionmentioning

confidence: 95%

Essential oils of Lippia sidoides and Mentha piperita against monogenean parasites and their influence on the hematology of Nile tilapia

et al. 2016

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“…Hashimoto et al (2016) also found low efficacy (1.9%) for 20 mg/L of the essential oil of L. sidoides against monogenoideans of Nile tilapia. On the other hand, exposure of C. auratus to extracts of Cinnamomum cassia, Lindera aggregata, Pseudolarix kaempferi, Caesalpinia sappan, Lysima chiachristinae, Cuscuta chinensis, Artemisia argyi and Eupatorium fortunei showed 100% efficacy against Dactylogyrus intermedius (Huang et al, 2013;Ji et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the chemical products involved cause death among fish because of their toxicity (Boijink et al, 2015;Steverding et al, 2005;Zhang et al, 2013;Zhang et al, 2014). Thus, many researchers' attention has been drawn towards seeking natural antiparasitic products (Boijink et al, 2015;Hashimoto et al, 2016;Huang et al, 2013;Ji et al, 2012;Steverding et al, 2005;Xiao-Feng et al, 2014;Zhang et al, 2013;Zhang et al, 2014;Zheng et al, 2015), since these could form an alternative to chemotherapeutic products.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, an extract from Euphorbia fischeriana showed 87.3% in vitro efficacy against Dactylogyrus vastator in Carassius auratus (Zhang et al, 2014). Although medicinal plants or their separate major chemical constituents have the advantage of low toxicity in comparison with chemotherapeutic products (Huang et al, 2013;Zhang et al, 2013;Zheng et al, 2015), the high concentrations obtained in in vitro trials cannot always be used in antiparasitic control and treatment among fish because of their toxicity (Hashimoto et al, 2016;Ji et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…These bioactive properties of the essential oil of L. alba are attributed to the richness of its major chemical constituents, such as geranial, carvone, neral, linalool and limonene. Recently, use of natural products derived from plants, for controlling parasites, has received great attention (Boijink et al, 2015;Hashimoto et al, 2016;Huang et al, 2013;Ji et al, 2012;Steverding et al, 2005;Xiao-Feng et al, 2014;Zhang et al, 2013;Zhang et al, 2014;Zheng et al, 2015). However, the effects of the essential oil of L. alba on ectoparasites of fish have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Soares

Neves²,

Oliveira

et al. 2016

Aquaculture

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This study investigated the in vivo and in vitro antiparasitic effects of the essential oil of Lippia alba and the bloodrelated and histopathological alterations that it causes in Colossoma macropomum. In the in vitro trial, the anthelminthic effects of 160, 320, 640, 1280 and 2560 mg/L of the essential oil were tested against monogenoideans (Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri) of the gills of this fish, which are its natural parasites. The concentrations of 1280 mg/L and 2560 mg/L showed 100% efficacy after 20 min of exposure to the essential oil, while at lower concentrations this efficacy against the gill monogenoideans only occurred after 2-3 h of in vitro exposure. However, in the controls, mortality of all of these monogenoideans only occurred after 9 h. A total of 240 fry were distributed into four treatments (20 fish per repetition) and three repetitions were used in the in vivo trial for baths with 100 and 150 mg/L of the essential oil of L. alba, for 30 min. The efficacy in this trial against Ichthyophthirius multifiliis in fish exposed to 100 and 150 mg/L of the essential oil was 40.7% and 50.3%, respectively. However, for monogenoideans, there was efficacy of 14.0% only in the fish exposed to 100 mg/L of the essential oil used. Moreover, the fish exposed to these concentrations of the essential oil presented increased plasma glucose levels, thus denoting signs of stress. Severe lesions such as hyperplasia, fusion of the lamellar epithelium, capillary dilatation, epithelial detachment, lamellar aneurysm, epithelial rupture with hemorrhage, congestion, edema, necrosis, mucous cell proliferation, chloride cells and lamellar hypertrophy were observed in the gills of the fish exposed to 100 and 150 mg/L of the essential oil of L. alba. Alterations to total protein levels, hemoglobin, hematocrit, red blood cell number, thrombocytes number, white blood cell number, lymphocytes, eosinophils and blood neutrophil number was also observed in these fish. The essential oil of L. alba showed great potential for antiparasitic treatment, given that it had high in vitro efficacy against monogenoideans and in vivo efficacy against the protozoon I. multifiliis. Because of the low concentrations of the essential oil (100 and 150 mg/L) that were tolerated by the fish and thus could be used in the therapeutic baths, the efficacy against monogenoideans was low. This indicates that there is a need for additional strategies for using this essential oil in antiparasitic treatments, since the concentrations that eliminate these ectoparasites are toxic for the hosts. Lastly, this was the first study on the antiparasitic activity of L. alba. Statement of Relevance The manuscript entitled "Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects", represents original article on use of the essential oil of Lippia alba on ectoparasites of tambaqui, an important finfish of Amazon regio...

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Use of Medicinal Plants in Aquaculture

Reverter

Tapissier‐Bontemps

Sasal

et al. 2017

Diagnosis and Control of Diseases of Fish and Shellfish

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Plants have been reported to produce various effects such as antistress, growth promotion, appetite stimulation, immunostimulation, aphrodisiac and to have antipathogen properties in fish and shrimp aquaculture due to their varied active principles such as alkaloids, terpenoids, tannins, saponins and flavonoids. To date, most scientific studies on the use of medicinal plants in aquaculture have focused on identification of biological activity rather than natural product determination. The plant species that have displayed the highest potential for use in aquaculture are garlic (Allium sativum), pomegranate (Punica granatum), bermuda grass (Cynodon dactylon), Indian ginseng (Whitania somnifera) and ginger (Zingiber officinale). Algae are considered to be a rich source of original bioactive molecules which display multiple bioactivities. In aquaculture, several recent studies have showed the potential of algae for the treatment of pathogens or to improve fish fitness.

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Screening of plant extracts for anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Cited by 32 publications

References 20 publications

Essential oils of Lippia sidoides and Mentha piperita against monogenean parasites and their influence on the hematology of Nile tilapia

Essential oils of Lippia sidoides and Mentha piperita against monogenean parasites and their influence on the hematology of Nile tilapia

Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects

Use of Medicinal Plants in Aquaculture

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