Screening of polyhydroxyalkanoates (PHA)-producing bacteria from soil bacteria strains

Ratnaningrum, Diah; Saraswaty, Vienna; Priatni, Sri; Lisdiyanti, Puspita; Purnomo, Adi Setyo; Pudjiraharti, Sri

doi:10.1088/1755-1315/277/1/012003

Cited by 9 publications

(11 citation statements)

References 14 publications

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“…Tan et al [2014a] demonstrated that, Nile blue staining provides an evidence for the production of PHAs which enabled microbe producers to be isolated and identified. Ratnaningrum et al [2019] stated that all the studied colonies confirmed to be able to present the cells that fluoresce under a fluorescent microscope.…”

Section: Results and Discussion Isolation And Screening Of The Pha Producing Bacteriamentioning

confidence: 83%

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Yasin¹,

Al-Mayaly²

2021

J. Ecol. Eng.

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Polyhydroxyalkanoates (PHAs) have gained much attention as biodegradable polymers, many efforts are being made to minimize the cost of PHAs by finding cheap carbon source depending on the type of microorganism and fermentation conditions. The aims of this study were to evaluate the effects of different glucose concentrations and other important conditions on the PHA production by Bacillus cereus isolated from soil. Polyhydroxyalkanoates PHAs accumulated by soil microorganisms were examined by screening the isolated bacteria using Sudan B Black and Nile Blue staining process. A Gram positive strain was identified using the 16s rRNA gene, deposited in the NCBI GenBank sequence database. Different growth conditions (favorite glucose concentrations 1-8 % (w/v), temperatures and pH) were tested and the growth parameters (sugar consumption, cell counting and Cell Dry Weight CDW) were studied. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer followed by a GC-MS analysis. The pure bacterial strain isolated from soil was deposited in the NCBI GenBank database B. cereus strain ARY73, which showed significant black colored granules (or dark blue) using Sudan B Black stain, it also showed positive to Nile blue A as a high indicator stain for PHA accumulation. B. cereus ARY73 showed high production of PHA using (w/v): 2% glucose and 1% nitrogen source at 35 °C and pH7 yields 79% per Cell Dry Weight and 96 h of incubation. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer confirming the PHA structure. The FTIR spectrophotometer, followed by a GC-MS analysis indicated the Scl-co-mcl PHA structure. This research demonstrates that the isolated strain B. cereus ARY73 was a good candidate for PHA production with a better quality for use in biomedical and other applications. The use of biopolymer in soil, enhanced the accumulation of the microorganisms (such as bacteria) capable of degrading biopolymer or biodegradation by-products yields by other species which were isolated in this study and demonstrated their efficiency in producing biopolymers.

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Section: Results and Discussion Isolation And Screening Of The Pha Producing Bacteriamentioning

confidence: 83%

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Yasin¹,

Al-Mayaly²

2021

J. Ecol. Eng.

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“…It is important to note that the production of PHB by Burkholderia sp. B73 was 52.9% in Ramsay's minimal medium (Ratnaningrum et al, 2019). In this study, we reveal that the yield of PHB obtained by using molasses as substrate was two times higher.…”

Section: Effect Of Initial Ph Value On Phb Yieldmentioning

confidence: 56%

“…1B). The data have been accomplished in the previous research (Ratnaningrum et al, 2019). Therefore, Burkholderia sp.…”

Section: Resultsmentioning

confidence: 99%

“…The confirmation of Burkholderia sp. B73 as PHB-producing bacteria has been done in previous research (Ratnaningrum et al, 2019). PHBproducing bacteria were observed based on Bhuwal et al (2013) andSpiekermann et al (1999) methods.…”

Section: Confirmation Of Burkholderia Sp B73 As Phb Producing Bacteriamentioning

confidence: 99%

“…Burkholderia sp. has been reported for the first time to produce a relatively high amount of PHB in synthetic media compared with other polyhydroxyalkanoates (PHA)-producing bacteria (Ratnaningrum et al, 2019). However, the effect of utilizing molasses for the production of PHB by Burkholderia sp.…”

Section: Introductionmentioning

confidence: 99%

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Harnessing molasses as a low-cost carbon source for production of poly-hydroxy butyrate (PHB) using Burkholderia sp. B73 bacteria

Ratnaningrum

Endah

Lisdiyanti

et al. 2021

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Burkholderia sp. has been reported as a poly-hydroxy-butyrate (PHB) producer. PHB is a natural polyester class with a wide range of applications in foods, medicines, and biomedicines. However, the high production cost of PHB may limit its potential. Molasses, a by-product of the sugarcane industry available abundantly, may be used as an alternative carbon source of PHB production. In this research, we aimed to evaluate PHB production by Burkholderia sp. B73 in fermentation media using molasses as an alternative carbon source. Small-scale experiments were performed in Erlenmeyer flasks on a shaker at 150 rpm and 30 °C to evaluate the best initial C/N ratio for biomass accumulation and PHB production. A set of parameters including bacterial growth, dry cell weight, yield, and FTIR spectrum of PHB were observed. The results showed that molasses could be used to grow Burkholderia sp. B73 and the highest PHB production was obtained when a 20:1 C/N ratio of molasses was applied in the fermentation medium. In addition, when the initial pH was adjusted to 7.0, the highest PHB yield was also produced. More importantly, the use of molasses as a carbon source improved the PHB yield by nearly 2-fold compared with our previous report using a synthetic Ramsay’s minimal medium. In conclusion, the experiment results showed that molasses could be used as a low-cost carbon source for PHB production by Burkholderia sp. B73 bacteria.

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Microbial Polyhydroxyalkanoates (PHAs): Efficient Replacement of Synthetic Polymers

et al. 2020

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Screening of polyhydroxyalkanoates (PHA)-producing bacteria from soil bacteria strains

Cited by 9 publications

References 14 publications

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Harnessing molasses as a low-cost carbon source for production of poly-hydroxy butyrate (PHB) using Burkholderia sp. B73 bacteria

Microbial Polyhydroxyalkanoates (PHAs): Efficient Replacement of Synthetic Polymers

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