Screening of proper leaf size in Centella asiatica for antioxidant potential and separation of phenolics using RP-HPLC

Tatmiya, Ritisha N.; Chudasama, Kiran S.; Jhala, Vibhuti M.; Thaker, Vrinda S.

doi:10.7324/japs.2014.40207

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…The biosynthesis of polyphenols which play an important role as antioxidant is a water soluble glycosylated flavonoids and mainly stored in epidermal cell vacuoles whereas the hydroxycinnamic acids are bound to cell walls in leaves (Meyer et al, 2006) (2013). Interestingly, those species promote better performance as compared to Centella asiatica (66.07 ± 4.66) that reported containing asiaticoside and poses high antioxidant activity (Tatmiya et al, 2014), and therefore were subjected to different solvents extraction for further analysis.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Bioactivities and Phytochemicals Content of Vegetables From Sabah, Malaysia

A. G.,

E.,

How

2016

BSJ

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This study aims to investigate potential of vegetables from Sabah with value-added benefits in nutraceuticals. Fifty-five samples of vegetables were collected from local market and tested for antioxidant activity using DPPH assay. Four species with high DPPH scavenging activity (>80%) which are Cosmos caudatus, Eryngium foetidum, Ipomoea batatas and Manihot esculenta Crantz were selected and subjected to different solvents extraction and tested to different scavenging assays (DPPH, O2 and NO), protein kinase-phosphatase assay (GSK-3β, MKK1, and MSG5) and antibacterial tests. Ethanol extract of I. batatas (90.56%), boiled water extract of M. esculenta Crantz (62.77%) and extractable polyphenol extract of E. foetidum (50.93%) exhibits comparable scavenging activities to catechin for DPPH, O2 and NO, respectively. Polyphenols, phenolic acids, flavonoids and proanthocynidins are detected in all extracts at concentration between 0.001 mg/g to 0.52 mg/g. The highest total polyphenols content (0.40±0.01 mg GAE/g), total phenolics content (0.52±0.01 mg GAE/g), total flavonoids content (0.13±0.01 mg CE/g) and total proanthocyanidins content (0.12±0 mg CE/g) were obtained in extractable polyphenols of Cosmos caudatus. No extracts were observed as inhibitor for GSK-3β, MKK1 and MSG5. Inhibition of Pseudomonas aeruginosa (8.0 mm to 12.3 mm) was only obtained in extractable polyphenols and ethanol extracts. Extractable polyphenols of E. foetidum exhibit the largest inhibition of Pseudomonas aeruginosa (12.3 mm).

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Section: Resultsmentioning

confidence: 99%

In Vitro Bioactivities and Phytochemicals Content of Vegetables From Sabah, Malaysia

A. G.,

E.,

How

2016

BSJ

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A linear model for leaf area measurement to screen potential leaf material for herbal drug in Adhatoda vasica L.

Thaker

Kuvad

Thaker

2016

JANS

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Leaf area is an important parameter in physiology and agronomy studies. Linear models for leaf area measurement are developed for plant species as a nondestructive method. The plant Adhatoda vasica L. (a medicinal plant) was selected and the leaves of this plant were used for development of linear model for leaf area using Leaf Area Meter (LAM) software. Planimetric parameters (length, length2, width and width2) and gravimetric (dry weight and water content) parameters are considered for the development of linear model for this plant species. Single factor ANOVA and linear correlations were worked out using these parameters and leaf area. The plant was showed significant relationship with the parameters studied. The best correlation as represented by regression coefficient (R2) was used and improved R2 is worked out. It is observed that with increase in leaf area, water content is also increased and showed best correlation with the leaf area. Thus water content can be taken as a parameter for developing linear model for leaf area is concluded.

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Screening of proper leaf size in Centella asiatica for antioxidant potential and separation of phenolics using RP-HPLC

Cited by 2 publications

References 8 publications

In Vitro Bioactivities and Phytochemicals Content of Vegetables From Sabah, Malaysia

In Vitro Bioactivities and Phytochemicals Content of Vegetables From Sabah, Malaysia

A linear model for leaf area measurement to screen potential leaf material for herbal drug in Adhatoda vasica L.

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