Screening, optimization and characterization of poly hydroxy butyrate from fresh water microalgal isolates

Kanaga, Selvaraj; Vishvanathan, Nandhini; Dandapani, Ramamurthy

doi:10.1080/24759651.2021.1926621

Cited by 10 publications

(4 citation statements)

References 65 publications

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“…The peaks from 1132 cm −1 to 826 cm −1 were associated with the stretching vibrations of C-O and C-C, while that at 1183 cm −1 was associated with the asymmetric stretching vibration of the C-O-C group. The peaks at 1300 and 1100 cm −1 correspond to the symmetric stretching of the C-O-C group, according to Heitmann et al (2016) [52], who reported the characterization of a nanostructured niobium oxyhydroxide dispersed PHB film, and Selvaraj et al ( 2021) [53].…”

Section: Ft-ir Spectrummentioning

confidence: 92%

Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using Bacillus megaterium ATCC 14581

Senila,

Gál,

Kovacs

et al. 2023

Polymers

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This study aimed to analyze the production of poly(3-hydroxybutyrate) (PHB) from lignocellulosic biomass through a series of steps, including microwave irradiation, ammonia delignification, enzymatic hydrolysis, and fermentation, using the Bacillus megaterium ATCC 14581 strain. The lignocellulosic biomass was first pretreated using microwave irradiation at different temperatures (180, 200, and 220 °C) for 10, 20, and 30 min. The optimal pretreatment conditions were determined using the central composite design (CCD) and the response surface methodology (RSM). In the second step, the pretreated biomass was subjected to ammonia delignification, followed by enzymatic hydrolysis. The yield obtained for the pretreated and enzymatically hydrolyzed biomass was lower (70.2%) compared to the pretreated, delignified, and enzymatically hydrolyzed biomass (91.4%). These hydrolysates were used as carbon substrates for the synthesis of PHB using Bacillus megaterium ATCC 14581 in batch cultures. Various analytical methods were employed, namely nuclear magnetic resonance (1H-NMR and13C-NMR), electrospray ionization mass spectrometry (EI-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA), to identify and characterize the extracted PHB. The XRD analysis confirmed the partially crystalline nature of PHB.

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Section: Ft-ir Spectrummentioning

confidence: 92%

Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using Bacillus megaterium ATCC 14581

Senila,

Gál,

Kovacs

et al. 2023

Polymers

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show abstract

“…In addition, Microcystis aeruginosa has been shown to exhibit PHB concentrations of up to 0.49 ± 0.5 mg/L [79]. Selvaraj et al [80] recorded maximum 80% PHB production by Chlorella sp. The highest recorded PHB content of 27% dry weight was achieved by Mehariya et al [81] during cultivation of Chlorococcum sp.…”

Section: Polyhydroxyalkanoatesmentioning

confidence: 99%

Green Synthesis of Bioplastics from Microalgae: A State-of-the-Art Review

Adetunji,

Erasmus

2024

Polymers

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The synthesis of conventional plastics has increased tremendously in the last decades due to rapid industrialization, population growth, and advancement in the use of modern technologies. However, overuse of these fossil fuel-based plastics has resulted in serious environmental and health hazards by causing pollution, global warming, etc. Therefore, the use of microalgae as a feedstock is a promising, green, and sustainable approach for the production of biobased plastics. Various biopolymers, such as polyhydroxybutyrate, polyurethane, polylactic acid, cellulose-based polymers, starch-based polymers, and protein-based polymers, can be produced from different strains of microalgae under varying culture conditions. Different techniques, including genetic engineering, metabolic engineering, the use of photobioreactors, response surface methodology, and artificial intelligence, are used to alter and improve microalgae stocks for the commercial synthesis of bioplastics at lower costs. In comparison to conventional plastics, these biobased plastics are biodegradable, biocompatible, recyclable, non-toxic, eco-friendly, and sustainable, with robust mechanical and thermoplastic properties. In addition, the bioplastics are suitable for a plethora of applications in the agriculture, construction, healthcare, electrical and electronics, and packaging industries. Thus, this review focuses on techniques for the production of biopolymers and bioplastics from microalgae. In addition, it discusses innovative and efficient strategies for large-scale bioplastic production while also providing insights into the life cycle assessment, end-of-life, and applications of bioplastics. Furthermore, some challenges affecting industrial scale bioplastics production and recommendations for future research are provided.

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“…Another study was done on chlorella species for PHB content. Results indicated it has 2.15 g/500 mL PHB content [ 39 ]; thus, there rises a need for gene-editing tools like CRISPR for modifying enzyme-producing PHB/PHA content in microalgae species for up-scaling bioplastic production.…”

Section: Modern Bioengineering Techniques To Enhance Production Of Bi...mentioning

confidence: 99%

“…According to previous reports, the maximum concentration (80%) of PHB is produced by Chlorella vulgaris [ 39 ]. A number of cyanobacteria species ( Arthrospira, Nostoc muscorum , Synechocystis PCC6803 and Synechococcus sp.…”

Section: Introductionmentioning

confidence: 99%

Microalgae in Bioplastic Production: A Comprehensive Review

Arora

Sharma

2023

Arab J Sci Eng

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The current era of industrialization includes a constantly increasing demand for plastic products, but because plastics are rarely recycled and are not biodegradable plastic pollution or “white pollution” has been the result. The consumption of petroleum-based plastics will be 20% of global annual oil by 2050, and thus there is an inevitable need to find an innovative solution to reduce plastic pollution. The biodegradable and environmentally benign bioplastics are suitable alternative to fossil-based plastics in the market due to sustainability, less carbon footprint, lower toxicity and high degradability rate. Microalgal species is an innovative approach to be explored and improved for bioplastic production. Microalgae are generally present in abundant quantity in our ecosystem, and polysaccharide in the algae can be processed and utilized to make biopolymers. Also, these species have a high growth rate and can be easily cultivated in wastewater streams. The review aims to determine the recent status of bioplastic production techniques from microalgal species and also reveal optimization opportunities involved in the process. Several strategies for bioplastic production from algal biomass are being discussed nowadays, and the most prominent are “with blending” (blending of algal biomass with bioplastics and starch) and “without blending” (microalgae as a feedstock for polyhydroxyalkanoates production). The advanced research on modern bioengineering techniques and well-established genetic tools like CRISPR–Cas9 should be encouraged to develop recombinant microalgae strains with elevated levels of PHA/PHB inside the cell.

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Screening, optimization and characterization of poly hydroxy butyrate from fresh water microalgal isolates

Cited by 10 publications

References 65 publications

Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using Bacillus megaterium ATCC 14581

Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using Bacillus megaterium ATCC 14581

Green Synthesis of Bioplastics from Microalgae: A State-of-the-Art Review

Microalgae in Bioplastic Production: A Comprehensive Review

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