Screening out the anti-insomnia components from Prunella vulgaris L. based on plasma pharmacochemistry combined with pharmacodynamic experiments and UPLC-MS/MS analysis

Lin, Tian-Feng; Qiu, Junna; Zhang, Shuang; Zhang, Yan; Sun, Meng; Zhang, Jinhua; Liu, Bin; Cheng, Fang; Jiang, Yanyan

doi:10.1016/j.jep.2021.114373

Cited by 14 publications

(14 citation statements)

References 31 publications

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“…Most medicinal herbs are orally administered, and the components absorbed into the blood are generally regarded as the potential active constituents. 13,14 However, little is known about the components of AVF that are absorbed into the blood. Network pharmacology, as an emerging area of pharmacology, offers a platform for exploring the interactions between components, disease, targets, and pathways.…”

Section: Introductionmentioning

confidence: 99%

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Integrating serum pharmacochemistry and network pharmacology to identify chemical markers for quality control of Apocyni Veneti Folium

Shi

Long

et al. 2022

Phytochemical Analysis

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Introduction: Apocyni Veneti Folium (AVF) is a commonly used traditional Chinese medicinal herb for the treatment of hypertension. Chemical markers are crucial for the quality control of herbal medicines; however, the therapeutic components of AVF remain to be well elucidated.Objectives: This study was intended to integrate serum pharmacochemistry and network pharmacology to identify chemical markers of AVF and establish an efficacy-related quality control method of AVF.Material and methods: Ultra-performance liquid chromatography quadrupole timeof-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) was applied to identify the absorbed AVF constituents in rat serum. Network pharmacology was further used to identify anti-hypertension-related chemical markers. Subsequently, a quantitative method was established using UPLC with diode array detection (DAD) and applied for quality evaluation of commercial AVF samples.Results: Thirteen prototype constituents were unequivocally or tentatively characterized in serum samples, among which quercetin, kaempferol, hyperoside, isoquercitrin, chlorogenic acid, cryptochlorogenic acid, and neochlorogenic acid were identified as dominant chemicals related to anti-hypertensive efficacy. The quantitative data showed that the total contents of seven marker components even showed 2-fold variation among 14 batches of commercial AVF samples with RSD values ranging from 12.15% to 75.61%. Hierarchical cluster analysis and heatmap analysis showed that 14 batches of commercial AVF samples could be divided into three main groups. Conclusion:The chemical markers obtained from this study could be applicable for efficacy-related quality control of AVF.

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Section: Introductionmentioning

confidence: 99%

“…Most medicinal herbs are orally administered, and the components absorbed into the blood are generally regarded as the potential active constituents 13,14 . However, little is known about the components of AVF that are absorbed into the blood.…”

Section: Introductionmentioning

confidence: 99%

Integrating serum pharmacochemistry and network pharmacology to identify chemical markers for quality control of Apocyni Veneti Folium

Shi

Long

et al. 2022

Phytochemical Analysis

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“…1 Modern phytochemical and pharmacological researches revealed that the major bioactive components of PS were phenolic acids, flavonoids, triterpenoids and saccharides [2][3][4] that are responsible for wide efficacies, such as anti-tumour, 5,6 anti-oxidative, 7,8 antiinflammatory, 9 anti-bacteria, 10 anti-virus 11,12 and sedative effects. 13 In Chinese Pharmacopoeia (2020 version), rosmarinic acid, a phenolic acid compound, was used as a single qualitative and quantitative marker for the quality control of PS with the specified content more than 0.2%. 1 However, the efficacy of medicinal herbs depends on its specific chemical composition, including chemical types, contents and proportions based on the "multicomponent and multitarget" property of medicinal herbs, 14 so rosmarinic acid alone should be inadequate for holistic quality control of PS, and other bioactive components also might be the potential quality control markers.…”

Section: Introductionmentioning

confidence: 99%

“…It is derived from the dried spica of Prunella vulgaris L., an annual herb that flowers and withers in summer, and is widely circulated throughout China 1 . Modern phytochemical and pharmacological researches revealed that the major bioactive components of PS were phenolic acids, flavonoids, triterpenoids and saccharides 2–4 that are responsible for wide efficacies, such as anti‐tumour, 5,6 anti‐oxidative, 7,8 anti‐inflammatory, 9 anti‐bacteria, 10 anti‐virus 11,12 and sedative effects 13 …”

Section: Introductionmentioning

confidence: 99%

Quality consistency evaluation of commercial Prunellae Spica by integrating determination of secondary metabolites and saccharides

Lin

Zhou

Hao

et al. 2022

Phytochemical Analysis

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Introduction: Prunellae Spica (PS) is a commonly used medicinal herb in China.Secondary metabolites and saccharides are major bioactive components of PS. However, holistic quality consistency of commercial PS is ambiguous due to lack of comprehensive evaluation methods and reliable quality control markers.Objectives: Integrating multiple chromatographic and chemometric methods to comprehensively evaluate the holistic quality of PS.Material and methods: Ultrahigh-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-TQ-MS/MS) was applied to quantify 12 secondary metabolites of PS. High-performance liquid chromatography coupled with photodiode array/evaporative light scattering detection (HPLC-PDA/ ELSD) and high-performance gel permeation chromatography (HPGPC) methods were used to characterise the saccharides. Multivariate statistical analysis was adopted to evaluate the quality consistency of commercial PS and explore the potential quality control markers. Results:The contents of secondary metabolites and saccharides were significantly different among commercial PS. All samples could be classified into three groups with ferulic acid, protocatechualdehyde, gallic acid, ursolic acid/oleanolic acid, sucrose, p-coumaric acid, chlorogenic acid as the major contributing components responsible for the difference. The content of rosmarinic acid was correlated with that of betulinic acid, hyperposide, chlorogenic acid, rutin, caffeic acid, p-coumaric acid and glucose, whereas polysaccharides, ferulic acid, protocatechualdehyde and ursolic acid/oleanolic acid, quercetin, sucrose and majority monosaccharides were not. Conclusion:The holistic quality of commercial PS was inconsistent. Together with rosmarinic acid, ferulic acid, protocatechualdehyde, ursolic acid/oleanolic acid, polysaccharides and sucrose might be recommended as potential quality control markers for the holistic quality control of PS.

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“…It is considered that mangiferin, salviaflaside, and rosmarinic acid are responsible for the anti-insomnia effects of P. vulgaris (Lin et al, 2021). P. vulgaris extracts have been shown to inhibit uterine tumor growth via the estrogen signaling and mitochondrionmediated apoptotic pathways (Lin et al, 2020).…”

mentioning

confidence: 99%

Analysis of the phytochemical components of Prunella vulgaris using high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry combined with molecular networking and assessment of their antioxidant and anti‐α‐glucosidase activities

Cao,

Li,

et al. 2023

Biomedical Chromatography

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Prunella vulgaris has long been used in traditional medicine and is consumed as a tea in China. Here, the total phenolic and flavonoid concentrations of plants from different geographical regions were measured. It was found that the total phenolic acid concentration ranged from 4.15 to 8.82 g of gallic acid equivalent per 100 g of dry weight (DW), and the total flavonoid concentration was 4.67–7.33 g of rutin equivalent per 100 g DW. Antioxidant activities were measured using 2,2‐diphenyl‐1‐picrylhydrazyl and 2,2′‐azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt, and the results ranged from 73.47% to 94.43% and 74.54% to 93.39%, respectively, whereas α‐glucosidase inhibition was between 75.31% and 95.49%. Correlation analysis showed that the total flavonoids in P. vulgaris had superior antioxidant and anti‐α‐glucosidase activities compared to the total phenolic compounds. The active components of P. vulgaris were analyzed using high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry combined with both classical molecular networking and feature‐based molecular networking on the Global Natural Products Social platform, identifying 32 compounds, namely 14 flavonoids, 12 phenolic compounds, and 6 other chemical components. These results could provide useful information on the use of P. vulgaris as a functional tea.

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Screening out the anti-insomnia components from Prunella vulgaris L. based on plasma pharmacochemistry combined with pharmacodynamic experiments and UPLC-MS/MS analysis

Cited by 14 publications

References 31 publications

Integrating serum pharmacochemistry and network pharmacology to identify chemical markers for quality control of Apocyni Veneti Folium

Integrating serum pharmacochemistry and network pharmacology to identify chemical markers for quality control of Apocyni Veneti Folium

Quality consistency evaluation of commercial Prunellae Spica by integrating determination of secondary metabolites and saccharides

Analysis of the phytochemical components of Prunella vulgaris using high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry combined with molecular networking and assessment of their antioxidant and anti‐α‐glucosidase activities

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