Screening System for the Maillard Reaction Inhibitor from Natural Product Extracts.

Matsuura, Nobuo; Aradate, Tadashi; Sasaki, Chihiro; Kojima, Hiroyuki; Ohara, Mitsuharu; Hasegawa, Junichi; Ubukata, Makoto

doi:10.1248/jhs.48.520

Cited by 155 publications

(96 citation statements)

References 33 publications

(36 reference statements)

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“…The formation of AGE was assessed using the fluorescence method (Matsuura et al, 2002) with some modifications. Briefly, 20 µL various concentrations MOE, amino guanidine (inhibitor), or phosphate buffer was added to the freshly prepared reaction mixture containing 400 µL 1 mg/mL bovine serum albumin (BSA) and 80 µL 1.0 M glucose in phosphate buffer, pH 7.4.…”

Section: Inhibition Of Age Formation Assaymentioning

confidence: 99%

Effect of Moringa oleifera on advanced glycation end-product formation and lipid metabolism gene expression in HepG2 cells

Sangkitikomol¹,

Rocejanasaroj²,

Tencomnao³

2014

Genet. Mol. Res.

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ABSTRACT. In Thai traditional medicine, Moringa oleifera is used for the treatment of diabetes and hyperlipidemia. Oxidative stress plays a major role in the pathogenesis of many degenerative diseases, such as hyperlipidemia, diabetes mellitus, and cardiovascular disease. We evaluated the antioxidant effect of M. oleifera extract (MOE) for reduction of advanced glycation end-product (AGE) formation, cell viability, oxidative stress, and lipid metabolism gene expression in HepG2 cells. We found that the lyophilized form of MOE in 80% ethanol possessed mean (± SD) total antioxidant, polyphenolic, and flavonoid contents of 9307 ± 364 TE mM/kg dry mass, 218 ± 1 GE mM/kg dry mass, and 286 ±12 QE mM/kg dry mass, determined using an oxygen radical absorbance capacity assay, a Folin Ciocalteu phenol assay, and a total flavonoids assay, respectively. Concentrations of 2.5-10.0 mg/ mL MOE could inhibit AGE-formation by 10-45%, and 100-1000 mg/L MOE reduced intracellular oxidative stress (P < 0.05) in a dose-dependent manner in the DCFH-DA assay. However, MOE induced cytotoxicity at high doses (2000-3000 mg/L), as shown by the MTT assay. MOE significantly downregulated the mRNA expression of the HMG-CoAR, PPARα1, and PPARγ genes (P < 0.05). We concluded that M. oleifera could have benefits for human health by reducing oxidative stress and AGE formation. Moreover, M. oleifera may reduce cholesterol and lipid synthesis by suppression of HMG-CoAR, PPARα1, and PPARγ gene expression, thereby maintaining lipid homeostasis.

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Section: Inhibition Of Age Formation Assaymentioning

confidence: 99%

Effect of Moringa oleifera on advanced glycation end-product formation and lipid metabolism gene expression in HepG2 cells

Sangkitikomol¹,

Rocejanasaroj²,

Tencomnao³

2014

Genet. Mol. Res.

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“…The evaluation of inhibitory activity against the Maillard reaction in vitro was performed according to the previously described method (Matsuura et al, 2002) with slight modification. The modeling of AGEs formation was between BSA and glucose.…”

Section: Bovine Serum Albumin (Bsa)-glucose Assay: Bsa-glucose Glycatmentioning

confidence: 99%

Protective effects of hesperidin derivatives and their stereoisomers against advanced glycation end-products formation

Mitsuhashi

Ubukata

2012

Pharmaceutical Biology

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“…The measurement of AGE formation and its inhibition by test compounds were performed according to Matsuura et al [17]. The inhibition of the Maillard reaction by the test samples was determined by the decrease in fluorescence in comparison with the positive control without the drug treatment.…”

Section: Age Inhibition Assaymentioning

confidence: 99%

Inhibition of Advanced Glycation End-Product Formation by Quercetin and Catechin: An Alternative Therapy for Treating Diabetic Complications

Julius

Hopper

2017

Asian J Pharm Clin Res

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Objective: The objective of this research was to determine early advanced glycation end-product (AGE) inhibition by natural aldose reductase inhibitors (ARIs), quercetin and catechin. Methods:The assay mixture (4 ml) consisted of 2 ml of 50 mM phosphate-buffered saline (pH 7.4), 50 µg/µl bovine serum albumin (BSA), and 2 mM glucose with or without the inhibitor. The test samples were treated with three different concentrations (10 mM, 20 mM, and 40 mM) of quercetin and catechin. High-throughput screening-based assay was adapted to perform the BSA-glucose test to determine the induction of AGE formation and its inhibition by quercetin, and catechin, using the fluorescence of the AGE-BSA sample at excitation and emission wavelengths of 350 and 450 nm. Result:The ARIs, quercetin and catechin inhibited early glycation with an inhibitory concentration value of 15.58 mM and 35.01 mM, respectively. Conclusion:The suppression of AGEs formation by natural inhibitors of aldose reductase would provide an alternative approach to the control of diabetic complications.

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Screening System for the Maillard Reaction Inhibitor from Natural Product Extracts.

Cited by 155 publications

References 33 publications

Effect of Moringa oleifera on advanced glycation end-product formation and lipid metabolism gene expression in HepG2 cells

Effect of Moringa oleifera on advanced glycation end-product formation and lipid metabolism gene expression in HepG2 cells

Protective effects of hesperidin derivatives and their stereoisomers against advanced glycation end-products formation

Inhibition of Advanced Glycation End-Product Formation by Quercetin and Catechin: An Alternative Therapy for Treating Diabetic Complications

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