Screening the bioactive compound from<i>Coptis chinensis</i>inflorescence by immobilized peroxisome proliferator‐activated receptor‐gamma

Sun, Jingjing; Zhang, Chaofeng

doi:10.1002/jssc.202101014

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…Research studies conducted by Sun and Hou also confirmed that PPAR-γ is the target of PAL. They immobilized PPAR-γ to screen the bioactive compounds fromCoptis chinensis inflorescence, and identified PAL as the bioactive compounds targeting PPARγthat can specifically bind to PPAR-γ [41,42]. In this study, we further analyzed the potential binding sites of PAL and PPAR-γ using molecular docking analysis, the results showed that The 325THR in PPAR-γ interact with PAL by forming a hydrogen bond.…”

Section: Discussionmentioning

confidence: 92%

PAL inhibits foam cell formation by regulating the PPAR-γ signaling pathway in oxidized low-density lipoprotein-induced THP-1 macrophages

Li,

Jia,

Mao

et al. 2023

Preprint

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Atherosclerotic cardiovascular disease continues to pose a major threat to human health. It has been shown that Chinese herbal medicines rich in palmatine(PAL) exert significant beneficial effects on atherosclerosis(AS). However, the specific biological functions and mechanisms of PAL in AS remain unclear. In this study, we investigated the effects of PAL on the core pathological changes in macrophage foam cell formation, a key process in the development of AS. The results demonstrated that PAL significantly reduced the formation of lipid droplets and accumulation of cholesterol ester in oxidized low-density lipoprotein (OX-LDL)-induced macrophage foam cells. RNA-sequencing was used to comprehensively analyze the effect of PAL. The findings revealed that the peroxisome proliferator-activated receptor-γ (PPAR-γ) pathway is the primary target of PAL in suppressing foam cell formation. Real-time fluorescence quantitative polymerase chain reaction and western blotting results confirmed that PAL substantially upregulated the transcription and expression levels of lipid metabolism-related proteins, such as PPAR-γ and liver X receptor-α (LXR-α), whereas it inhibited the transcription of inflammatory factors TNF-α, interleukin-1β (IL-1β), and IL-6. These biological changes were reversed by treatment with the PPAR-γ inhibitor T0070907. Molecular docking analysis showed that the binding sites of PAL with PPAR-γ (325THR) is close to the reported PPAR-γ agonist rosiglitazone（323HIS）, suggesting that PAL may act as a PPAR-γ partial agonist to improve atherosclerosis. Collectively, the results of this study suggest that PAL promotes cholesterol efflux and inhibits inflammatory responses mainly by regulating the PPAR-γ signaling pathway, thus ameliorating macrophage foam cell formation. Our findings may provide a foundation for further research on the pharmacological activity and potential value of PAL in the treatment of AS.

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Section: Discussionmentioning

confidence: 92%

PAL inhibits foam cell formation by regulating the PPAR-γ signaling pathway in oxidized low-density lipoprotein-induced THP-1 macrophages

Li,

Jia,

Mao

et al. 2023

Preprint

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Quality Assessment of Corydalis saxicola Bunting Using Quantitative Analysis of Multi‐Components by Single Marker and Fingerprint Analysis

Shen,

Yang,

Feng

et al. 2024

J of Separation Science

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Yan Huanglian is the whole grass and rhizome of Corydalis saxicola Bunting (CSB) of the Papaveraceae family. CSB has various pharmacological effects and is a promising ethnopharmaceutical. The chromatographic fingerprint of 16 batches of CSB samples was established in this study. Quantitative analysis of multi‐components by single marker and the external standard method (ESM) was used to quantitatively assess seven constituents of CSB. Because of its high stability and availability, dehydrocavidine was used as a reference to determine the relative correction factors (RCFs) of columbamine, epiberberine, coptisine hydrochloride, palmatine chloride, berberine hydrochloride, and chelerythrine based on high‐performance liquid chromatography. The calculated quantitative analysis of multi‐components by single marker values is consistent with those obtained from the ESM, and the repeatability of the RCFs was demonstrated. For the first time, the quantitative analysis of multi‐components by the single marker method has been established for the simultaneous determination of seven components, and it is demonstrated to be feasible and accurate for the quality evaluation of CSB combined with chromatographic fingerprint analysis. The combination of these methods could be used as a reference for quality control in Chinese medicine.

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Berberine hydrochloride ameliorates PM2.5-induced pulmonary fibrosis in mice through inhibiting oxidative stress and inflammatory

Zhao,

Ma,

et al. 2023

Chemico-Biological Interactions

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Screening the bioactive compound fromCoptis chinensisinflorescence by immobilized peroxisome proliferator‐activated receptor‐gamma

Cited by 4 publications

References 30 publications

PAL inhibits foam cell formation by regulating the PPAR-γ signaling pathway in oxidized low-density lipoprotein-induced THP-1 macrophages

PAL inhibits foam cell formation by regulating the PPAR-γ signaling pathway in oxidized low-density lipoprotein-induced THP-1 macrophages

Quality Assessment of Corydalis saxicola Bunting Using Quantitative Analysis of Multi‐Components by Single Marker and Fingerprint Analysis

Berberine hydrochloride ameliorates PM2.5-induced pulmonary fibrosis in mice through inhibiting oxidative stress and inflammatory

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