Screening the Marker Components in Psoralea corylifolia L. with the Aids of Spectrum-Effect Relationship and Component Knock-Out by UPLC-MS2

Abstract

Psoralea corylifolia L., (P. corylifolia), which is used for treating vitiligo in clinic, shows inhibitory and activating effects on tyrosinase, a rate-limiting enzyme of melanogenesis. This study aimed to determine the active ingredients in the ethenal extracts of P. corylifolia on tyrosinase activity. The spectrum-effect relationship and knock-out method were established to predict the active compounds. Their structures were then identified with the high resolution mass spectra. A high performance liquid chromatography method was established to obtain the specific chromatograms. Tyrosinase activity in vitro was assayed by the method of oxidation rate of levodopa. Partial least squares method was used to test the spectrum-effect relationships. Chromatographic peaks P2, P4, P9, P10, P11, P13, P21, P26, P28, and P30 were positively related to the activating effects on tyrosinase activity in PE, whereas chromatographic peaks P1, P3, P6, P14, P16, P19, P22, and P29 were negatively related to the activating effects on tyrosinase in the P. corylifolia (PEs). When the sample concentration was 0.5 g·mL−1, equal to the amount of raw medicinal herbs, the target components were daidzein (P2), psoralen (P5), neobavaisoflavone (P13), and psoralidin (P20), which were consistent with the results of spectrum-effect relationships.

Pharmacological Activities of Psoralidin: A Comprehensive Review of the Molecular Mechanisms of Action

“…Patients with mild forms of vitiligo showed maximum improvement in 1-10 months, while chronic cases with lip vitiligo showed an inadequate response. Exposure of the patient to sunlight (5-30 min daily) with oral administration of this for 1-7 weeks gave improved the beneficial effects (Sharma et al, 2005). Possible mechanism of action: PSO interacts with ultraviolet in the epidermis forming photosensitive fragments of DNA.…”

Dynamic changes of secondary metabolites and tyrosinase activity of Malus pumila flowers

“…A 3-factor 3-level orthogonal experiment was designed and statistically analyzed by SPSS 19.0 [24]. The investigated levels of each factor were selected depending on the above experiment results of the single-factor.…”

Anti-Hepatoma Compound Determination by the Method of Spectrum Effect Relationship, Component Knock-Out, and UPLC-MS2 in Scheflera heptaphylla (L.)Frodin Harms and Its Mechanism

“…“Spectrum-Effect Relationship” and “Component Knock-Out” technology has been successfully applied in screening the active ingredients of natural products ( Shi et al, 2018 and Li et al, 2019 ). In this paper, partial least square method was used to analyze the correlation between 10 quantitative characteristic chromatographic peaks of S. heptaphylla and the anti-hepatoma activity (IC 50 value) of S. heptaphylla .…”