Screening wild Lycopersicon species for resistance to powdery mildew (Oidium lycoperiscum)

Lindhout, P.; Pet, G.; Beek, H. van der

doi:10.1007/bf00023771

Cited by 53 publications

(33 citation statements)

References 2 publications

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“…Oidium neolycopersici was collected from infected tomato plants in the Netherlands (Lindhout et al 1994a) and is continuously maintained on S-MM plants in growth chambers at 20 ± 2°C, relative humidity (RH) 70% and 16 h day-length. Fresh spores were washed from seriously infected leaves with water to prepare the inoculum (2 · 10 4 spores/ml).…”

Section: Fungal Materials and Inoculum Preparationmentioning

confidence: 99%

Tomato defense to the powdery mildew fungus: differences in expression of genes in susceptible, monogenic- and polygenic resistance responses are mainly in timing

Bai²,

Jacobsen³

et al. 2006

Plant Mol Biol

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Oidium neolycopersici is a causal agent of tomato powdery mildew. In this paper, gene expression profiles were investigated of susceptible, monogenicand polygenic resistant tomato genotypes in response to O. neolycopersici infection by using cDNA-AFLP. Around 30,000 TDFs (Transcript Derived Fragments), representing~22% of the transcriptome based on in silico estimation, were identified and 887 TDFs were differentially expressed (DE-TDFs) upon inoculation with O. neolycopersici spores. Forty-two percent of the identified DE-TDFs were detected in both the compatible and incompatible interactions, a subset of these were studied for their temporal patterns. All of these common induced DE-TDFs displayed an expression peak at 7 days post incoluation in monogenic resistant response but sustained up-regulation in the susceptible and the polygenic resistant response. While more than half of these common DE-TDFs showed earlier timing in incompatible interactions compared to compatible interaction. Only 2% of the identified DE-TDFs were specific to either the monogenic or the polygenic resistant response. By annotation of the 230 sequenced DE-TDFs we found that 34% of the corresponding transcripts were known to be involved in plant defense, whereas the other transcripts played general roles in signal transduction (11%), regulation (24%), protein synthesis and degradation (11%), energy metabolism (12%) including photosynthesis, photorespiration and respiration.

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Section: Fungal Materials and Inoculum Preparationmentioning

confidence: 99%

Tomato defense to the powdery mildew fungus: differences in expression of genes in susceptible, monogenic- and polygenic resistance responses are mainly in timing

Bai²,

Jacobsen³

et al. 2006

Plant Mol Biol

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“…Research in the last 15 years has broadened knowledge of the genetic basis of resistance to O. neolycopersici (Lindhout et al 1994b;Ciccarese et al 1998;Huang et al 2000b;Bai et al 2003Bai et al , 2004Bai et al , 2005Li et al 2006Li et al , 2007Li et al , 2008Li et al , 2012Seifi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

et al. 2013

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Tomato powdery mildew (Oidium neolycopersici) is one of the most devastating diseases of cultivated tomatoes worldwide. Although the first epidemics were recorded more than 25 years ago many aspects of this host-pathogen interaction are still not well understood. Detailed morphological and molecular studies of the anamorphs confirmed that O. neolycopersici is phylogeneticaly close to Erysiphe aquilegiae var. ranunculi. Host range is rather broad, apart from Solanaceae hosts were found in the families Apocynaceae, Campanulaceae, Crassulaceae, Cistaceae, Cucurbitaceae, Linaceae, Malvaceae, Papaveraceae, Pedialiaceae, Scrophulariaceae, Valerianaceae a Violaceae. Non-host resistance within these families is not based on inhibition of formation of primary haustorium, however, on post-haustorial hypersensitive reponse and another type of non-hypersensitive resistance. Screening of wild Solanum species (previous Lycopersicon spp.) germplasm revealed valuable sources of resistance (S. habrochaites, S. pennellii, S. cheesmaniae, S. chilense, S. peruvianum). The main resistance mechanism was found to be a hypersensitive response (HR), in some cases followed by limited development of the pathogen. However, there is a broad variation in resistance response on the histological and cytological level. Interaction between many wild Solanum spp. and O. neolycopersici is race-specific, at least three races were differentiated. In some interspecific crosses (S. lycopersicum × S. habrochaites) adult plant resistance was observed. Biochemical studies focusing on production of reactive oxygen species (ROS) and peroxidase activity during infection of O. neolycopersici showed that production of ROS and activity of corresponding enzymes is related to activation of defence responses in genotypes of wild Solanum sect. Lycopersicon. The significance of nitric oxide (NO) in O. neolycopersici pathogenesis was supported by experiments with NO donors and scavengers. In moderately resistant genotype S. chmielewskii, treatment by heat stress caused slight deceleration of pathogen development, increased production of jasmonic acid (JA) and abscisic acid (ABA) and increased peroxidase activity in infected plants. The different degree of tomato resistance/ susceptibility did not markedly change the rate and extent of photosynthetic response to O. neolycopersici; only minimal impairment of photosynthesis was found in both susceptible and moderately resistant genotypes during the Harper Adams University, Newport, Shropshire TF10 8NB, UK first 9 days after inoculation. The accumulated evidence confirm a crucial role of localised increased production of ROS and reactive nitrogen species (RNS) in response to pathogen penetration into plant tissue and its involvement in the plant resistance responses including the initiation and progression of plant cell death in host wild Solanum species. Crucial points of further research are discussed.

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“…Complete resistance was observed in accession LA 1775 of L. hirsutum tested against a local population of O. lycopersici in Poland (Kozik, 1993). Moderate resistance occurred in L. parviflorum and L. pennellii (Kozik, 1993;Lindhout et al, 1994). Kozik (1993) reported moderate resistance in several L. esculentum selections.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and inheritance of resistance to powdery mildew (Oidium lycopersici) in Lycopersicon species

et al. 1998

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Among 146 accessions of Lycopersicon pimpinellifolium, 132 of L. esculentum var. cerasiforme and 53 of L. peruvianum screened for resistance to powdery mildew, caused by Oidium lycopersici, a wide variation in reactions was found. Two plants of L. esculentum var. cerasiforme accession LA-1230 were resistant. One resistant symptomless plant of accession LA-1230, designated LC-95, produced homozygous resistant progenies. LC-95 was crossed with cv. Marmande (susceptible parent) and F 1 , F 2 and backcrosses to the resistant and the susceptible parents were derived. These genotypes were grown in glasshouses at 23ЊC and 95-100% RH and inoculated with O. lycopersici. The F 1 plants were susceptible. F 2 and backcross segregations fitted the hypothesis of a single recessive gene which is here designated ol-2.

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Screening wild Lycopersicon species for resistance to powdery mildew (Oidium lycoperiscum)

Cited by 53 publications

References 2 publications

Tomato defense to the powdery mildew fungus: differences in expression of genes in susceptible, monogenic- and polygenic resistance responses are mainly in timing

Tomato defense to the powdery mildew fungus: differences in expression of genes in susceptible, monogenic- and polygenic resistance responses are mainly in timing

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

Occurrence and inheritance of resistance to powdery mildew (Oidium lycopersici) in Lycopersicon species

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