Sct1 functions in partnership with Cdc10 in a transcription complex that activates cell cycle START and inhibits differentiation

Caligiuri, Maureen; Beach, David

doi:10.1016/0092-8674(93)90079-6

Cited by 114 publications

(162 citation statements)

References 43 publications

Supporting

Mentioning

159

Contrasting

Order By: Relevance

“…Consistent with our previous results, resl -and res2-spores generated from resl +Iresl -res2+1res2-diploid cells germinated and formed colonies at 30°C with 45 and 97% efficiencies, respectively. On the contrary, resl -res2- (Tanaka et al, 1992) and the recent biochemical data support this implication (Caligiuri and Beach, 1993). We examined the ability of res2+ to rescue temperature-sensitive cdc10-129 and res2+ controls the cell cycle…”

Section: Resultsmentioning

confidence: 99%

“…Res2 markedly differs from ResI in relationship to Cdc10. The Res protein has been implicated to act in association with CdclO (Tanaka et al, 1992;Caligiuri and Beach, 1993 Figure 4 and Table I; Tanaka et al, 1992 (Table Ill).…”

Section: Discussionmentioning

confidence: 99%

“…We have identified a new cdc gene called resl + from S.pombe (Tanaka et al, 1992); Caligiuri and Beach (1993) identified the same gene independently. This gene encodes a protein with two copies of the Swi/ankyrin motif and significant similarity to Cdc10, Swi4 and Swi6.…”

mentioning

confidence: 99%

See 2 more Smart Citations

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

Miyamoto¹,

Tanaka²,

Okayama³

1994

The EMBO Journal

135

View full text Add to dashboard Cite

In the fission yeast Schizosaccharomyces pombe, the cdc10+ and res1+ genes play a crucial role in the start of mitotic and meiotic cycles. They encode structurally related transcriptional complex proteins and regulate some S phase‐specific genes. Here we report the identification of a new member of this family named as res2+. res2+ has been isolated as a multicopy suppressor of a res1‐ null mutant and specifies a 73 kDa protein, which has two copies of the Swi/ankyrin motif and shares the highest sequence and structure similarity with the Res1 protein. res2+ is largely redundant in function with res1+ and is required for the initiation of mitotic and premeiotic DNA synthesis, but has an additional role in meiotic division. Unlike res1+, res2+ is highly induced during conjugation and strongly depends on cdc10+ for its activity. We conclude that the fission yeast contains two functionally overlapping parallel ‘start’ systems, Res1‐Cdc10 and Res2‐Cdc10, the former of which plays a major role in mitotic cycle whereas the latter in meiotic cycle.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

Miyamoto¹,

Tanaka²,

Okayama³

1994

The EMBO Journal

135

View full text Add to dashboard Cite

show abstract

“…ste9 Mutants Enter the M-Phase from the Pre-Start G 1 -Phase As described above, ste9 ϩ is essential for the proper regulation of cell cycle progression in the G 1 -phase. The Cdc10 protein, in combination with Res1 or Res2, functions as a transcription factor for the genes that are essential for transition from the G 1 to the S-phase (Lowndes et al, 1992;Tanaka et al, 1992;Caligiuri and Beach, 1993;Miyamoto et al, 1994). At the restrictive temperature, cdc10 ts mutants arrest in the pre-Start period of the G 1 -phase (Nurse et al, 1976;Nurse and Bissett, 1981).…”

Section: Ste9 and Wee1 Mutations Cause Synthetic Lethalitymentioning

confidence: 99%

Fission Yeast Ste9, a Homolog of Hct1/Cdh1 and Fizzy-related, Is a Novel Negative Regulator of Cell Cycle Progression during G₁-Phase

Kitamura

Maekawa

Shimoda

1998

MBoC

110

125

View full text Add to dashboard Cite

When proliferating fission yeast cells are exposed to nitrogen starvation, they initiate conjugation and differentiate into ascospores. Cell cycle arrest in the G1-phase is one of the prerequisites for cell differentiation, because conjugation occurs only in the pre-Start G1-phase. The role ofste9 + in the cell cycle progression was investigated. Ste9 is a WD-repeat protein that is highly homologous to Hct1/Cdh1 and Fizzy-related. The ste9 mutants were sterile because they were defective in cell cycle arrest in the G1-phase upon starvation. Sterility was partially suppressed by the mutation in cig2 that encoded the major G1/S cyclin. Although cells lacking Ste9 function grow normally, the ste9 mutation was synthetically lethal with the wee1 mutation. In the double mutants ofste9 cdc10 ts, cells arrested in G1-phase at the restrictive temperature, but the level of mitotic cyclin (Cdc13) did not decrease. In these cells, abortive mitosis occurred from the pre-Start G1-phase. Overexpression of Ste9 decreased the Cdc13 protein level and the H1-histone kinase activity. In these cells, mitosis was inhibited and an extra round of DNA replication occurred. Ste9 regulates G1 progression possibly by controlling the amount of the mitotic cyclin in the G1-phase.

show abstract

“…Among them, we can find the cyclin-dependent kinase, Cdc2 1 and the activity of the transcription factor MBF, which is a multimeric complex whose core proteins are Cdc10, Res1 and Res2. [2][3][4] MBF, which is the functional analog of mammalian pRB/E2F, drives the G1-to-S wave of transcription, controlling the expression of some genes that are directly or indirectly required for DNA synthesis, such as cdc18 (the fission yeast homolog to CDC6), cdt1 and the gene coding for ribonucleotide reductase, cdc22. 3,5,6 We have shown that while cig2 is one of the genes that is under the control of MBF, the protein encoded by this gene, the cyclin Cig2, is also part of a negative feed-back loop that phosphorylates and inhibits MBF.…”

Section: Introductionmentioning

confidence: 99%

A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave

et al. 2016

View full text Add to dashboard Cite

The Schizosaccharomyces pombe MBF complex activates the transcription of genes required for DNA synthesis and S phase. The MBF complex contains several proteins, including the core components Cdc10, Res1 and Res2, the co-repressor proteins Yox1 and Nrm1 and the co-activator Rep2. It has recently been shown how MBF is regulated when either the DNA damage or the DNA synthesis checkpoints are activated. However, how MBF is regulated in a normal unperturbed cell cycle is still not well understood. We have set up a genome-wide genomic screen searching for global regulators of MBF. We have crossed our knock-out collection library with a reporter strain that allows the measurement of MBF activity in live cells by flow cytometry. We confirm previously known regulators of MBF and show that COP9/ signalosome and tRNA methyltransferases also regulate MBF activity.

show abstract

Sct1 functions in partnership with Cdc10 in a transcription complex that activates cell cycle START and inhibits differentiation

Cited by 114 publications

References 43 publications

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

Fission Yeast Ste9, a Homolog of Hct1/Cdh1 and Fizzy-related, Is a Novel Negative Regulator of Cell Cycle Progression during G₁-Phase

A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave

Contact Info

Product

Resources

About

Sct1 functions in partnership with Cdc10 in a transcription complex that activates cell cycle START and inhibits differentiation

Cited by 114 publications

References 43 publications

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

Fission Yeast Ste9, a Homolog of Hct1/Cdh1 and Fizzy-related, Is a Novel Negative Regulator of Cell Cycle Progression during G1-Phase

A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave

Contact Info

Product

Resources

About

Fission Yeast Ste9, a Homolog of Hct1/Cdh1 and Fizzy-related, Is a Novel Negative Regulator of Cell Cycle Progression during G₁-Phase