Scutellaria orientalis subsp. virens ve Scutellaria salviifolia üzerinde Anatomik, Mikromorfolojik, Karyolojik ve Biyokimyasal bir çalışma

Abstract

In this study, the anatomical and micromorphological structure, karyological characteristics and biochemical content of Scutellaria orientalis subsp. virens and endemic Scutellaria salviifolia, whose distributions areas overlap, were compared. Some anatomical and micromorphological differences were observed on the taxa; scleranchymatic pericycle layer on the stem, stomata density, distribution of trichomes, as well as the main vascular bundle and general shape of the petiole. The chromosome numbers of both taxa were determined as 2n = 22. However, there was a difference between chromosome length range and total chromosome length. The chromosome numbers and chromosome morphologies of these species have been defined for the first time in this paper. Differences in biochemical content were observed between species. Chlorophyll a (Chl a), total chlorophyll (Total Chl), total carbohydrate and malondialdehyde (MDA) contents were determined higher in leaf and stem samples of S. orientalis subsp. virens than S. salviifolia. There was no significant difference between the two taxa in terms of chlorophyll b (Chl b) content. Carotenoid (Car) content was detected higher in leaves samples of S. orientalis subsp. virens, but no significant difference was found between stems samples. Also, the effect of taxa on biochemical contents in relation to the habitat they live in is given in this study.

Karyotype report for eight <i>Euphorbia</i> taxa cultivated in Turkey

“…The seeds in Petri dishes are sprout at 25 C on wet filter paper. The root tips are pretreated with 0.05% colchicine for 2 h at room temperature before being fixed with glacial acetic acid-absolute ethanol (1 : 3) for at least 24 h at 4 C. The materials are then rinsed in tap water for 3 times after being hydrolyzed in 1 M HCl for 10 min at 60 C. The components are then mounted in 45% acetic acid after being stained in Feulgen reagent for 1 h (Elci 1982, Acar et al 2022. Photographs of the metaphase chromosomes of the examined species were taken under a Nikon E200 microscope with, a Nikon Digital Sight DS Fi2 microscope camera.…”