SDF-1α loaded bioengineered human amniotic membrane-derived scaffold transplantation in combination with hyperbaric oxygen improved diabetic wound healing

Nasiry, Davood; Khalatbary, Ali Reza; Abdollahifar, Mohammad‐Amin; Bayat, Mohammad; Amini, Abdollah; Ashtiani, Mohammad Kazemi; Rajabi, Sarah; Noori, Afshin; Piryaei, Abbas

doi:10.1016/j.jbiosc.2022.01.012

Cited by 29 publications

(16 citation statements)

References 39 publications

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“…In the present study, in order to accurately evaluate the interventions performed in diabetic wound healing, evaluations were performed in three time periods. The seventh day was due to the overlap between the inflammatory and proliferative phases, the fourteenth day was due to the overlap of the proliferative phases and the beginning of the regeneration phase, and the 21st day was due to the peak of tissue regeneration and maturation [10,11,13]. Prolonged inflammation is one of the most important challenges in the chronic wound healing process [35].…”

Section: Discussionmentioning

confidence: 99%

“…The progressive changes in the wound closure were photographed on days 0, 7, 14, and 21 after surgery, using a digital camera (FinePix S20, Japan). Then, the photos were analyzed using MacBiophotonics in ImageJ software (National Institutes of Health) and wound closure rate was calculated and compared between the groups on each time point [ 13 ], using the following formula: …”

Section: Methodsmentioning

confidence: 99%

“…For this purpose, a total of 25 randomly picked micrographs from 5 sections were captured for each sample using a 40 × magnification objective lens. The collagen percentage was calculated by dividing the total blue-colored area by the total areas of the micrographs [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…Then, qRT-PCR reactions were performed for three biological replicates on the real-time PCR system (Applied Biosystems StepOne instrument) using SYBR Green Master Mix and sets of primers (Table 1 ). The final analyses were performed using the comparative CT method (2 −ΔΔct ) [ 13 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…The main and important factor in the occurrence of angiogenesis is vascular endothelial growth factor (VEGF), which is produced by the local cells of the wound [ 6 ]. In diabetic wounds, due to the reasons mentioned earlier, the production of this cytokine faces a serious problem [ 12 , 13 ]. It seems that if it is possible to use a combination that has properties such as availability, anti-inflammatory, antioxidant and antimicrobial, it can be helped to a great extent in the healing of diabetic wounds [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing

Nasiry

Khalatbary

Ghaemi

et al. 2022

BMC Complement Med Ther

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Background Diabetic wounds are one of the most important issues in diabetic patients. It seems that Juglans regia L. leaf with antioxidant and anti-inflammatory potentials can be profitable for healing of diabetic wounds. The aim of present study was to investigate the topical administration of Juglans regia L. leaf extract in diabetic wound healing. Methods Seventy-five diabetic male rats were randomly divided into 5 groups (n = 15), including: untreated (Control) group, Eucerin group, 2% Juglans regia L. ointment (JRL 2%) group, 5% Juglans regia L. ointment (JRL 5%) group, and Phenytoin group as a reference drug. Sampling was performed at days 7, 14, and 21 after surgery. Evaluation tests included stereology, immunohistochemistry, molecular, and biomechanical. Results Our results showed that the wound closure rate, volumes of newly formed of epidermis and dermis, density of fibroblasts and blood vessels, collagen deposition, density of proliferation cells, expression levels of TGF-β and VEGF genes, and biomechanical characteristics were significantly higher in extract groups compared to control and eucerin groups, however, these changes were considerable in the JRL 5% group (P < 0.05). This is while that the density of neutrophils and expression levels of TNF-α and IL-1β genes in the extract groups, especially in the JRL 5% group, were significantly reduced compared to control and eucerin groups (P < 0.05). Conclusion Topical administration of Juglans regia L. leaf extract, especially in 5% concentration, considerably accelerates diabetic wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%