Search for alkaloids on callus culture of Passiflora alata

Machado, Michelli Wesz; Neto, Carlos Stern; Salgado, Juliana Mota; Zaffari, Gilmar Roberto; Barison, Andersson; Campos, Francinete Ramos; Corilo, Yuri; Eberlin, Marcos N.; Biavatti, Maique W.

doi:10.1590/s1516-89132010000400020

Cited by 16 publications

(9 citation statements)

References 28 publications

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“…9 Moreover, LC-MS targeted analysis (i.e., analysis of specific metabolites) may be a powerful tool for plant metabolomics studies. 10 This work focuses on targeted analysis leading to the identification of further β-carboline alkaloids from sweet (P. alata) and sour (P. edulis) pulp and seeds by UHPLC-MS techniques and the quantification of harmane and harmine in sweet fruits by SBSE(PDMS)-LC/Flu.…”

Section: Introductionmentioning

confidence: 99%

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

Freire

Silva

Yariwake

2017

J. Braz. Chem. Soc.

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Samples of pulp and seeds of sour (P. edulis) and sweet (P. alata) passionfruit were subjected to targeted analysis to identify β-carboline alkaloids, using combined UHPLC-MS (ultrahigh performance liquid chromatography-mass spectrometry) and SBSE(PDMS) (stir bar sorption extraction using polydimethylsiloxane as stationary phase) for sample preconcentration. All the samples contained harmane and harmine; harmaline was found only in P. alata seeds and in P. edulis pulp, while harmalol was found only in P. alata seeds. An analysis of sweet passionfruit by SBSE(PDMS) combined with LC/Flu (liquid chromatography/fluorescence detection) indicated 1.03 × 10 -1 ± 3.05 × 10 -3 µg harmane L -1 pulp and 7.44 × 10 -5 ± 2.55 × 10 -6 µg harmane g -1 seeds, but harmine was not detectable by the quantitative method. Our findings underscore the need for further studies about passionfruit food safety, in view of the potential toxicity of β-carboline alkaloids.

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Section: Introductionmentioning

confidence: 99%

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

Freire

Silva

Yariwake

2017

J. Braz. Chem. Soc.

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“…The explants reacted differently depending on the hormonal balance and physico-chemical factors. It has been found that callus induction occurs both in the photoperiod (16/8 h light / dark) (Antognoni et al, 2007;Machado et al, 2010;Pinto et al, 2011;Rocha et al, 2012), as well as in total darkness (da Silva et al, 2009;Carvalho et al, 2013) cited by Ozarowski, 2011. Using leaf fragments from P. quadrangularis, the callus was efficiently induced on the base medium MS supplemented with BA (2.0 mg/l) and GA3 (1.0 mg/l) but also in the case of the variant containing only BA (0.5 mg/l).…”

Section: Callusogenesismentioning

confidence: 99%

In Vitro Plant Tissue Culture: Means for Production of Passiflora Species

Paula¹,

Buru²,

Cătană³

et al. 2020

IJIAAR

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Passiflora genus includes over 600 species native to tropical and subtropical areas of America, appreciated for the production of fruit and medicinal value. Their ornamental potential is especially appreciated in North America and in Europe. With the expansion of the flower trade and the use of secondary metabolites in the pharmaceutical industry, a need for the constant monopolization of new technologies and alternative in vitro techniques that allow to obtain a uniform, high quality material free of pests and diseases occurs. Passiflora's tissue cultures began to be studied in 1966, raising more and more interest of researchers worldwide.Depending on the source and type of the explant, plant growth regulators, and the used genotype, direct and indirect organogenesis are the main regeneration pathways for Passiflora. The latest approaches regarding the choice of explant and its source, the plant material surface sterilization and the specific requirements of each micropropagation stage are presented within our review. To this genus, the reduced gas exchange of in vitro growing of seedlings has been shown as the main cause of lack of success. In this regard, for regeneration and obtained improvements in morphogenesis, different protocols have been developed by using inhibitors of ethylene. In recent years, studies suggest that via somatic embryogenesis, starting from mature and immature zygotic embryos, regenerated plants that have maintained their mother plant ploidy can be successfully obtained. This confirms the callus cultures as main path to obtain in vitro regenerated Passiflora plants.

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“…Additionally, alkaloids also exhibit autophagic effects. β‐Carboline (harmol) is isolated from Peganum harmala , Passiflora edulis, and Passiflora incarnate (Machado et al, ). These plants are used in traditional medicine for treatment of asthma, and a recent study revealed that this alkaloid exhibits cytotoxicity toward cancer cells (Khan, Maalik, Iqbal, & Malik, ).…”

Section: Natural Product‐derived Compounds As Potent Inducers Of Cellmentioning

confidence: 99%

Molecular mechanisms of natural compounds in cell death induction and sensitization to chemotherapeutic drugs in lung cancer

Wattanathamsan

Hayakawa

Pongrakhananon

2019

Phytotherapy Research

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Cancer remains one of the leading causes of death worldwide, especially lung cancer.Chemotherapeutic drugs are commonly used for lung cancer treatment; nonetheless, undesirable side effects and drug resistance remain major challenges for therapeutic success. Therefore, harmless and effective treatments against lung cancer are urgently required. The use of natural phytochemical products, in single or combinatorial therapy, is an emerging strategy for prevention and cure of cancer because of the various remarkable anticancer properties of these compounds. Cell death, which primarily occurs via apoptosis and nonapoptotic mechanisms (necrosis, autophagy, and cellular senescence), is one of the antineoplastic effects of natural compounds. In this review, we highlight representative plant-derived compounds with cancer chemopreventive and sensitizing effects in combination with chemotherapeutic drugs with various cell death-inducing mechanisms. Relevant molecular mechanisms implicated in the pharmacological effects of these natural compounds are discussed. Overall, this review provides a reference and new perspective for application of phytochemical agents as potential anti-lung cancer drugs for further cancer drug research and development.

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Search for alkaloids on callus culture of Passiflora alata

Cited by 16 publications

References 28 publications

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

In Vitro Plant Tissue Culture: Means for Production of Passiflora Species

Molecular mechanisms of natural compounds in cell death induction and sensitization to chemotherapeutic drugs in lung cancer

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