Searching for New Targets and Treatments in the Battle Against Squamous Cell Carcinoma of the Head and Neck, with Specific Focus on Tumours of the Tongue

Sgaramella, Nicola; Gu, Xiaolian; Boldrup, Linda; Coates, Philip J.; Fåhræus, Robin; Califano, Luigi; Tartaro, Gianpaolo; Colella, Giuseppe; Spaak, Lena Norberg; Strom, Adrian; Wilms, Torben; Muzio, Lorenzo Lo; Orabona, Giovanni Dell’Aversana; Santagata, Mario; Loljung, Lotta; Rossiello, R.; Danielsson, Karin; Strindlund, Klas; Lillqvist, Sandra; Nylander, Karin

doi:10.2174/1568026618666180116121624

Cited by 19 publications

(20 citation statements)

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“…despite substantial efforts to develop effective anticancer therapies, the clinical outcomes of patients with TScc remain unsatisfactory because of the characteristic high malignancy rate of TScc (6,7). Over the past few decades, there were no significant improvements in the 5-year survival rate of patients with TScc, and the morbidity associated with TScc has been increasing every year (8). consequently, elucidation of the complicated pathogenesis of TScc may aid in devising novel effective therapeutic approaches and improving the prognosis of patients with TScc.…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Zou

et al. 2020

Int J Mol Med

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A long non-coding RNA (lncRNA) called prostate cancer-associated non-coding RNA 1 (PRNCR1) serves crucial roles in the aggressive phenotypes of colorectal cancer and non-small cell lung cancer. However, there is little research on the expression profile, clinical value and detailed functions of PRNCR1 in tongue squamous cell carcinoma (TScc). The aim of the present study was to determine PRNCR1 expression in TScc and to examine the involvement of PRNCR1 in TScc progression. The molecular mechanisms behind the oncogenic effects of PRNCR1 in TScc cells were also investigated. PRNCR1 was revealed to be upregulated in TScc tumors and cell lines. The high PRNCR1 expression showed a significant correlation with tumor size, clinical stage, lymph node metastasis, and shorter overall survival times among patients with TScc. A PRNCR1-knockdown reduced TScc cell proliferation, migration and invasion, and increased apoptosis in vitro. Additionally, the PRNCR1-knockdown slowed down in vivo tumor growth of TScc cells. With regards to the mechanism, PRNCR1 acted as a competing endogenous RNA on microRNA-944 (miR-944) in TScc cells, and the effects of the PRNCR1-knockdown were reversed by an miR-944-knockdown. HOXB5 was validated as a direct target gene of miR-944 in TScc cells, and HOXB5 expression was found to be positively regulated by PRNCR1. Furthermore, resumption of HOXB5 expression reversed the tumor-suppressive actions of miR-944 in TScc cells. In conclusion, PRNCR1 acts as an oncogenic lncRNA in TScc through the upregulation of HOXB5 by sponging miR-944, thereby indicating a potential therapeutic target in TScc.

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Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Zou

et al. 2020

Int J Mol Med

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“…Although advanced diagnostic strategies and therapies, including surgery, chemotherapy and radiotherapy, are widely used, the possibility of complete recovery is remote (8,9). Therefore, a complete recognition of the molecular mechanisms involved in the multi-step processes of TSCC carcinogenesis and progression could provide more effective therapeutic targets for TSCC treatment (10,11).…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA LINC00152 acts as a sponge of miRNA‑193b‑3p to promote tongue squamous cell carcinoma progression

Rui

Cao

et al. 2020

Oncol Lett

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Dysregulated expression of long non-coding RNAs has been determined to be important in cancer development; however, their role in tongue squamous cell carcinoma (TSCC) progression and carcinogenesis, to the best of our knowledge, is yet to be elucidated. The present study revealed that long intergenic non-coding RNA 00152 (LINC00152) expression was significantly increased in human TSCC tissues compared with in tissues from matched controls using RT-qPCR. In TSCC cell lines, CAL-27 and SCC-9, LINC00152 was revealed to promote TSCC cell proliferation, enhance cell cycle progression and inhibit cell apoptosis. Additionally, migration and invasion of TSCC cell lines was increased in response to LINC00152 overexpression. Mechanistically, LINC00152 was determined to be localized in the cytoplasm and acted as a microRNA (miR)-193b-3p sponge, and LINC00152 knockdown or miR-193b-3p mimics both inhibited PI3K signaling pathway activation and downstream AKT phosphorylation; therefore, promoting TSCC progression in vitro. Overall, the results of the present study suggested that increased LINC00152 expression in TSCC tissues may act as a sponge of miR-193b-3p to promote cancer progression in vitro.

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“…1). All 25 tumours analysed expressed MUC1, with 11 tumours presenting low levels (QS 1-5) and 14 presenting medium levels (QS [6][7][8][9][10][11][12]. No tumour had a QS >12 (Table I).…”

Section: Methodsmentioning

confidence: 99%

“…Elevated MUC1 serum level is associated with shorter disease-free survival and overall survival time in patients with breast cancer (17). Since MUC1 is one of the most highly upregulated genes in tumour-free tongue tissues (7), it could be used as a potential marker of so-called 'field changes' in SCCOT. These changes could be due to pre-neoplastic genetic events or be indicative of environmental alterations predisposing to tumour formation (18,19).…”

Section: Introductionmentioning

confidence: 99%

Levels of MUC1 in tumours and serum of patients with different sub‑types of squamous cell carcinoma of the head and neck

Boldrup

Coates

et al. 2020

Oncol Lett

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Mucin 1 (MUC1) is a membrane-bound and secreted glycoprotein that has a protective role in surface epithelia. We recently demonstrated that MUC1 mRNA expression was upregulated in tumour-free tongue tissues adjacent to squamous cell carcinoma of the oral tongue (SCCOT) compared with that in the tumour tissues. The present study investigated MUC1 protein in SCCOT tissue and serum from patients with squamous cell carcinoma of the head and neck (SCCHN) at different sub-sites. The results from immunohistochemistry demonstrated that all SCCOT tissues expressed MUC1; however, the protein levels were not correlated with MUC1 mRNA levels in the same tumours. Furthermore, serum MUC1 level was lower in patients with SCCOT, tonsil SCC and gingival SCC compared with that in healthy subjects; however, the difference was only significant for patients with SCCOT (P=0.0421). No correlation was seen between MUC1 level in tumour tissues and MUCI level in serum from the same patients. The absence of correlation between MUC1 protein and mRNA levels in SCCOT tissues emphasized the importance of validating genomic data in clinical samples. Although significant MUC1 downregulation was observed in the serum of patients with SCCOT, there was a large variation within the groups, suggesting that MUC1 may not be used as a biomarker for these types of tumors.

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Searching for New Targets and Treatments in the Battle Against Squamous Cell Carcinoma of the Head and Neck, with Specific Focus on Tumours of the Tongue

Cited by 19 publications

References 0 publications

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Long non‑coding RNA LINC00152 acts as a sponge of miRNA‑193b‑3p to promote tongue squamous cell carcinoma progression

Levels of MUC1 in tumours and serum of patients with different sub‑types of squamous cell carcinoma of the head and neck

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