Seasonal changes in the protein profile and enzyme activity of the epididymal luminal fluid in the lizard, Eutropis carinata (Schneider, 1801)

Medini, R.; Bhagya, Mahadevaiah; Samson, S.

doi:10.1163/15707563-17000124

Cited by 4 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the annual reproductive cycle, the epididymis of the lizard E. carinata undergoes dramatic changes, both morphologically and biochemically, that occur in a well-organized sequence. Enzymes, such as acid phosphatase, alkaline phosphatase, and α-glucosidase, are highly sensitive to seasonal changes and their activity parallels the production of epididymal proteins 29,[56][57][58] . Beta-hexosaminidase (Hex) is the major lysosomal enzyme associated with the event of fertilization.…”

Section: Discussionmentioning

confidence: 99%

Epididymis of the Lizard <i>Eutropis carinata</i>: A Light Microscopic and Ultrastructural Seasonal Study

Aranha

Bhagya

Sagar

2023

JER

Self Cite

View full text Add to dashboard Cite

The epididymis of the lizard Eutropis carinata can be divided into four regions viz., an initial segment (extra testicular) Efferent ductules (Eds) and three regions, the anterior region, a broad middle, and a narrow posterior, comparable to the caput, corpus, and cauda epididymidis of mammals. The anterior region of the epididymis is closely associated with a whitish initial segment (extra testicular), the Efferent ductules (Eds). The epididymal wall consists of an epithelial layer lining the lumen resting on a basement membrane surrounded by four to five layers of smooth circular muscles, a layer of connective tissue, and a layer of serosa. The ultrastructure study of the initial segment of epididymis the Eds during breeding season discloses four cell types in its epithelium viz., ciliated, non-ciliated, Apical-Mitochondria Rich Cells (AMRC), and basal cells. The anterior, middle, and posterior regions of the epididymis show five different cell types namely principal, basal, AMRC, narrow, and clear cells. Ciliated and non-ciliated cells are limited to the initial segment of the epididymis while, principal, narrow, and clear cells are found in the rest of the three regions of the epididymis. Basal and AMRC are found in all four regions. AMRCs are the most abundant cell type in the initial segment while principal cells are the major components of the epithelial lining of the remaining regions of the epididymis. During the non-breeding season, all the cell types are present but with regression and altered cytology of the cells without any sign of cellular activity in the different regions of the epididymis. During the breeding season, even the circulating testosterone levels are significantly higher compared to the non-breeding season. This is the first report describing different cell types in the initial segment and three different regions of the epididymis with ultrastructural seasonal variations in the Keeled Indian Mabuya, Eutropis carinata (Scincidae).

show abstract

Section: Discussionmentioning

confidence: 99%

Epididymis of the Lizard <i>Eutropis carinata</i>: A Light Microscopic and Ultrastructural Seasonal Study

Aranha

Bhagya

Sagar

2023

JER

Self Cite

View full text Add to dashboard Cite

show abstract

“…The wall of the epididymal duct in E. perditus and U. vautieri is lined by a simple cylindrical epithelium with secretory vesicles. In Eutropis carinata , Medini et al (2018) found that these secretory vesicles present in the epididymis of some lizards can play an important role in the maturation of sperm while stored in this organ. These vesicles are similar to that reported in other Squamatas: T. torquatus (Silva et al, 2016), E. nanuzae (Melo et al, 2019), Sitana ponticeriana (Akbarsha et al, 2006), Podarcis sicula (Desantis et al, 2002) and S. pygaea (Sever, 2010).…”

Section: Discussionmentioning

confidence: 99%

Histological study and immunohistochemical location of cytoskeletal proteins in the testis and epididymis of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

et al. 2022

View full text Add to dashboard Cite

The objective of the study was to histologically describe the structure of the testis and epididymis, identifying the GAGs in them. Furthermore, the distribution of smooth muscle alpha‐actin, desmin, and vimentin is described of E. bilineatus, E. perditus, and U. vautieri, through immunohistochemical method. Two adult males of each species of the lizards were collected in southeastern Brazil. In the testicular albuginea, the presence of neutral glycoproteins was detected. In E. bilineatus, the epididymal duct epithelium is simple, composed of secretory cells, cylindrical in shape and acidophilic cytoplasm. In the testis of the three lizards there was a positive immunolocation accentuated for α‐SMA in the dense connective tissue of the tunica albuginea. A moderate reaction was observed in the connective tissue albuginous and in the layer of smooth muscle fibers in a circumferential arrangement of the epididymal duct. The immunolocation for desmin was accentuated in the fibers of the testicular albuginea of E. bilineatus, while in U. vautier and E. perditus was moderate. In the epididymis, only in E. perditus and E. bilineatus positive immunoreaction was verified. In the three lizards there were no observations of immunostaining for vimentin with the antibody utilized.

show abstract

“…Certain enzymes, such as acid phosphatase and alkaline phosphatase, are highly sensitive to seasonal changes, and their activity coincides with the production of proteins in epididymis. The number of protein fractions present in epididymal lumen is the highest during the breeding season [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of the Season and Region Factor on Phosphoproteome of Stallion Epididymal Sperm

Dyrda

Orzołek

Ner‐Kluza

et al. 2021

Animals

View full text Add to dashboard Cite

Epididymal maturation can be defined as a scope of changes occurring during epididymal transit that prepare spermatozoa to undergo capacitation. One of the most common post-translational modifications involved in the sperm maturation process and their ability to fertilise an oocyte is the phosphorylation of sperm proteins. The aim of this study was to compare tyrosine, serine, and threonine phosphorylation patterns of sperm proteins isolated from three subsequent segments of the stallion epididymis, during and out of the breeding season. Intensities of phosphorylation signals and phosphoproteins profiles varied in consecutive regions of the epididymis. However, significant differences in the phosphorylation status were demonstrated in case of endoplasmic reticulum chaperone BiP (75 and 32 kDa), protein disulfide-isomerase A3 (50 kDa), nesprin-1 (23 kDa), peroxiredoxin-5 (17 kDa), and protein bicaudal D homolog (15 kDa) for season x type of phosphorylated residues variables. Significant differences in the phosphorylation status were also demonstrated in case of endoplasmic reticulum chaperone BiP and albumin (61 kDa), protein disulfide-isomerase A3 (50 kDa), and protein bicaudal D homolog (15 kDa) for region x type of phosphorylated residues variables.

show abstract

Seasonal changes in the protein profile and enzyme activity of the epididymal luminal fluid in the lizard, Eutropis carinata (Schneider, 1801)

Cited by 4 publications

References 44 publications

Epididymis of the Lizard <i>Eutropis carinata</i>: A Light Microscopic and Ultrastructural Seasonal Study

Epididymis of the Lizard <i>Eutropis carinata</i>: A Light Microscopic and Ultrastructural Seasonal Study

Histological study and immunohistochemical location of cytoskeletal proteins in the testis and epididymis of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

Influence of the Season and Region Factor on Phosphoproteome of Stallion Epididymal Sperm

Contact Info

Product

Resources

About