Advanced driving assistance systems (ADAS) are primarily designed to increase driving safety and reduce traffic congestion without paying too much attention to passenger comfort or motion sickness. However, in view of autonomous cars, and taking into account that the lack of comfort and motion sickness increase in passengers, analysis from a comfort perspective is essential in the future car investigation. The aim of this work is to study in detail how passenger’s comfort evaluation parameters vary depending on the driving style, car or road. The database used has been developed by compiling the accelerations suffered by passengers when three drivers cruise two different vehicles on different types of routes. In order to evaluate both comfort and motion sickness, first, the numerical values of the main comfort evaluation variables reported in the literature have been analyzed. Moreover, a complementary statistical analysis of probability density and a power spectral analysis are performed. Finally, quantitative results are compared with passenger qualitative feedback. The results show the high dependence of comfort evaluation variables’ value with the road type. In addition, it has been demonstrated that the driving style and vehicle dynamics amplify or attenuate those values. Additionally, it has been demonstrated that contributions from longitudinal and lateral accelerations have a much greater effect in the lack of comfort than vertical ones. Finally, based on the concrete results obtained, a new experimental campaign is proposed.