Second metabolite content and antioxidative, α-amylase inhibitory activity of Phellinus gilvus fruiting body extracts

Abstract

Fractions yielded from Phellinus gilvus fruiting bodies by liquid-liquid partition of methanol extract contained various bioactive compounds such as phenolics, flavonoids and terpenes. Butanol, water and ethyl acetate fractions had high content of total phenolics, 158.12 – 247.31 mg of gallic acid equivalents (GAE)/g and low content of flavonoids, 21.74 - 36.05 mg of quercetin equivalents (QE)/g, respectively. These values highly correlated with their antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging potential with IC50 values of 84.5 -108.44 μg/mL and reducing power. These fractions showed moderate α-amylase inhibitory activity with IC50 values of 4.77, 10.69 and 3.9 mg/mL respectively. The P. gilvus fractions exhibited very slight inhibitory activity on the growth of Gram-positive and Gram-negative bacterial strains at the concentration of 20 mg/mL. The results suggest the utility of butanol, water, and ethyl acetate fractions from fruiting bodies of P. gilvus collected in Vietnam for further characterization in order to apply for diabetes or anti-aging prevention.

Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses

“…Various anticancer and anticholinesterase agents have been detected in F. torulosa , which display antibacterial, antifungal, antioxidant, and cytotoxic activities (Covino et al 2019 ; Deveci et al 2019 ). Fuscoporia gilva also exhibits various bioactivities, displaying potential for diabetes control and improved immunity (Sun et al 2020 ; Duong and Dang 2022 ). However, F. gilva has been reported as “ Phellinus gilvus ” in some studies, despite the species combination of Wagner and Fischer ( 2002 ).…”

“…A relatively high number of ITS GenBank sequences is present for another macro-fungal taxon, Fuscoporia ( Hymenochaetales , Basidiomycota ), as ITS is commonly used to identify Fuscoporia species, especially in non-taxonomic studies (Covino et al 2019 ; Noji et al 2021 ). Precise identification of Fuscoporia species is crucial because some species, such as F. gilva and F. torulosa , are being intensively studied for their medicinal effects (Deveci et al 2019 ; Duong and Dang 2022 ). Incorrect species identification may cause confusion in establishing accurate species profiling for medicinal and biotechnological applications.…”