Secondary Ag/AgCl Pseudo-Reference Electrode on Silicon Substrate

Abstract

Amperometric analysis systems with two or three require a potential of reference electrode stable in function of time. In this work we developed an Ag/AgCl pseudo-reference onto gold film on silicon substrate. The amperometric electrodeposition method utilized (constant potential) provided a uniform deposited film and a controlled process characterization. It allows us to obtain electrodes with excellent performance during fourteen consecutives day. The characterization in function of the chloride concentration and time were also presented.

Investigation of the signaling mechanism and verification of the performance of an electrochemical real-time PCR system based on the interaction of methylene blue with DNA

“…A pseudo-reference Ag/AgCl electrode was fabricated on the gold reference electrode patterned on a glass chip by silver and silver chloride deposition. 33,34 A cleaning step was performed with the potential cycling from 0 to 1.3 V with 0.3 V s À1 scan rate for 2 h in 10 mM H 2 SO 4 . Electro-deposition of the silver layer was then conducted in 2 mM AgNO 3 by applying reduction potential (0 to À0.4 V) under a 0.5 mV s À1 scan rate in the presence of a additional platinum counter electrode and Ag/ AgCl reference electrode.…”

Silver Chloride/Ferricyanide-Based Quasi-Reference Electrode for Potentiometric Sensing Applications

“…Ag/AgCl quasi-reference electrodes (QREs) are described in [29][30][31][32]. With all the convenience and functionality of Ag/AgCl QREs, their serious drawback is the non-stability of Ag/AgCl measuring surface from the environment in which it is located.…”

“…Encapsulation of Ag/AgCl/Clinto strong hardening polymers such as polyvinyl chloride [51,52] or poly (n-butyl acrylate) [53][54][55] is described in recent studies. SSREs described in [26,[31][32][33]35] keep the potential constant in a solution containing [Fe(CN) 6 ] 3-/4-, but protective polymer layers, in some cases, create a barrier for the penetration of substances generating the analytical signal to the measuring surface. Additionally, the complexity of such electrodes' manufacturing makes their mass use unlikely.…”

“…Figure 2a: Ag 0 (s) + Cl -(aq.) → AgCl (s) + eat E > 0.05 V[31,32];• Figure 2b: 4Ag 0 (s) + [Fe(CN) 6 ] 4-(aq.) → Ag 4 [Fe(CN) 6 ] (s) + 4e -(first peak at 0.05 V < Е< 0.15 V) and [Fe(CN) 6 ] 4-(s) → [Fe(CN) 6 ] 3-(s) + e -(second peak at Е> 0.15 V), which is associated with a similar process for an indifferent Pt [Fe(CN) 6 ] 4-(aq.)…”

Desenvolvimento de um sensor eletroquímico planar modificado com 1-2 Diaminobenzeno (DAB) para monitoração de nitrito por FIA-automatizada.

“…Na extremidade desse tubo há uma membrana porosa l (SCE), Hg/Hg 2 Cl 2 (1 mol L -1 ) de KCl (Calomel), Hg/Hg 2 SO 4 , g/HgO e Ag/AgCl. Estes são exemplos de eletrodos de referência secundários mais c ][32] [33]. Eles são mais robustos e homogêneos, Em sistemas de medição com dois ou três eletrodos é fundamental a utilização de um eletrodo de referência com potencial estável para garantir uma medição confiável pelo eletrodo de trabalho [28].…”