Secondary vascularised hairy flap transfer for eyebrow reconstruction

Mizuno, Hiroshi; Akaishi, Satoshi; Kobe, Kyoko

doi:10.1016/j.bjps.2008.11.113

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…These stem cells have been characterized at length to differentiate along multiple lineage pathways useful in soft tissue repair [6]. The implantation of hASCs with various other materials has begun to expand the usefulness of soft tissue engineering as reconstructive options [7-9]; however, problems with engineered adipose tissue retention, graft persistence, and neovascularization remain.…”

Section: Introductionmentioning

confidence: 99%

Engineering vascularized soft tissue flaps in an animal model using human adipose–derived stem cells and VEGF+PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle

et al. 2015

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Insufficient neovascularization is associated with high levels of resorption and necrosis in autologous and engineered fat grafts. We tested the hypothesis that incorporating angiogenic growth factor into a scaffold–stem cell construct and implanting this construct around a vascular pedicle improves neovascularization and adipogenesis for engineering soft tissue flaps. Poly(lactic-co-glycolic-acid/polyethylene glycol (PLGA/PEG) microspheres containing vascular endothelial growth factor (VEGF) were impregnated into collagen-chitosan scaffolds seeded with human adipose-derived stem cells (hASCs). This setup was analyzed in vitro and then implanted into isolated chambers around a discrete vascular pedicle in nude rats. Engineered tissue samples within the chambers were harvested and analyzed for differences in vascularization and adipose tissue growth. In vitro testing showed that the collagen-chitosan scaffold provided a supportive environment for hASC integration and proliferation. PLGA/PEG microspheres with slow-release VEGF had no negative effect on cell survival in collagen-chitosan scaffolds. In vivo, the system resulted in a statistically significant increase in neovascularization that in turn led to a significant increase in adipose tissue persistence after 8 weeks versus control constructs. These data indicate that our model—hASCs integrated with a collagen-chitosan scaffold incorporated with VEGF-containing PLGA/PEG microspheres supported by a predominant vascular vessel inside a chamber—provides a promising, clinically translatable platform for engineering vascularized soft tissue flap. The engineered adipose tissue with a vascular pedicle could conceivably be transferred as a vascularized soft tissue pedicle flap or free flap to a recipient site for the repair of soft-tissue defects.

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Section: Introductionmentioning

confidence: 99%

Engineering vascularized soft tissue flaps in an animal model using human adipose–derived stem cells and VEGF+PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle

et al. 2015

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Ophriopoïèse

Baraër¹,

Darsonval

Lejeune³

et al. 2013

Annales de Chirurgie Plastique Esthétique

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The eyebrow is an essential anatomical area, from a social point of view, so its reconstruction, in case of skin defect, must be as meticulous as possible, with the less residual sequela. Capillary density extremely varies from one person to another and the different methods of restoration of this area should absolutely take this into consideration. We are going to review the various techniques of reconstruction, according to the sex and the surface to cover.

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Supraorbital Arter Ada Flebi ile Kaş Rekonstrüksiyonu

Kılınç¹

2012

JIUMF

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Secondary vascularised hairy flap transfer for eyebrow reconstruction

Cited by 3 publications

References 5 publications

Engineering vascularized soft tissue flaps in an animal model using human adipose–derived stem cells and VEGF+PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle

Engineering vascularized soft tissue flaps in an animal model using human adipose–derived stem cells and VEGF+PLGA/PEG microspheres on a collagen-chitosan scaffold with a flow-through vascular pedicle

Ophriopoïèse

Supraorbital Arter Ada Flebi ile Kaş Rekonstrüksiyonu

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