Secretion of human leukocyte antigen-G by human embryos is associated with a higher in vitro fertilization pregnancy rate

Yie, Shang-mian; Bałakier, Hanna; Motamedi, Gelareh; Librach, Clifford L.

doi:10.1016/j.fertnstert.2004.06.059

Cited by 101 publications

(74 citation statements)

References 32 publications

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“…In the study presented here, a traditional Day 3 morphology compared to the sHLA-G expression plus a GES-score in order to identify embryos for transfer resulting into favorable clinical and ongoing pregnancy rates. These findings are supported by a number of researchers [20,[23][24][25][26]. Furthermore, we reported a decrease of fetal loss, in the sHLA-G positive group, between 6 week and 12 week ultrasound, a finding supported by Rebmann et al [27].…”

Section: Treatment Groups Percent Of Fetusses Lost (%)supporting

confidence: 88%

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Kotzé

Hansen

Keskintepe

et al. 2010

J Assist Reprod Genet

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Section: Treatment Groups Percent Of Fetusses Lost (%)supporting

confidence: 88%

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Kotzé

Hansen

Keskintepe

et al. 2010

J Assist Reprod Genet

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“…In the other, no pregnancies were obtained in 26 of 66 women whose transferred embryos did not secrete soluble HLA-G, while nine pregnancies occurred in the 40 of 66 women whose embryos did secrete soluble HLA-G (8). It has also been reported that soluble HLA-G secretion was independent of embryo grade, but the cleavage rate of embryos secreting soluble HLA-G was significantly higher than that of those lacking it, as was the live birth rate (9). A prospective study, in which at least one embryo known to be producing soluble HLA-G was transferred to the mother, has also shown significantly improved implantation and pregnancy rates (10).…”

Section: Discussionmentioning

confidence: 94%

Differential Expression of Alternatively Spliced Transcripts of HLA-G in Human Preimplantation Embryos and Inner Cell Masses

Yao

Barlow

Sargent

2005

The Journal of Immunology

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It has been reported that preimplantation human embryos secrete HLA-G, and the levels may be predictive of their ability to implant. However, it is not known which of the membrane-bound (HLA-G 1–4) and soluble (HLA-G 5–6) alternatively spliced forms are present, nor the developmental stage at which they appear. Therefore, we have investigated HLA-G mRNA isoform expression on single embryos at the two-, four-, six-, and eight-cell, morula, and blastocyst stages. The percentage of embryos expressing each HLA-G isoform mRNA increased with developmental stage, but contrary to expectation, HLA-G5 mRNA was not detected in single two- to eight-cell embryos and was only expressed by 20% of morulae and blastocysts. Similarly, soluble HLA-G6 mRNA was not detected until the blastocyst stage and then in only one-third of embryos. In contrast, labeling with MEM G/9 Ab (specific for HLA-G1 and -G5) was observed in 15 of 20 two- to eight-cell embryos and 5 of 5 blastocysts. This disparity between mRNA and protein may be due to HLA-G protein remaining from maternal oocyte stores produced before embryonic genome activation and brings into question the measurement of soluble HLA-G for clinical evaluation of embryo quality. Although HLA-G is expressed in the preimplantation embryo, later it is primarily expressed in the invasive trophoblast of the placenta rather than the fetus. Therefore, we have investigated whether down-regulation of HLA-G first occurs in the inner cell mass (precursor fetal cells) of the blastocyst and, in support of this concept, have shown the absence HLA-G1 and -G5 protein and mRNA.

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“…This has been associated with increased cleavage rates and successful implantation of the embryo during in vitro fertilization (IVF) treatment (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). In IVF protocols, embryo selection for transfer to the uterus is based on visual assessment of embryo morphology and cleavage rate.…”

mentioning

confidence: 99%

Analysis of HLA-G in Maternal Plasma, Follicular Fluid, and Preimplantation Embryos Reveal an Asymmetric Pattern of Expression

Shaikly

Morrison

Taranissi³

et al. 2008

The Journal of Immunology

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Soluble HLA-G (sHLA-G) secretion by human preimplantation embryos in culture has been associated with successful embryo development, and therefore has potential to serve as a noninvasive marker of embryo viability. We have examined the spatial and temporal expression of HLA-G in embryos of varying developmental competence and the role of maternal factors in human embryonic HLA-G expression. Embryos that reached blastocyst stage on day 5 showed a higher frequency of sHLA-G secretion than those at morula or arrested stages (p < 0.05). There was no significant difference in sHLA-G secretion between normal embryos and those diagnosed as chromosomally abnormal by preimplantation genetic diagnosis. HLA-G detected in maternal plasma and follicular fluid did not appear to correlate with HLA-G expressed in the embryo or embryo supernatants. Confocal microscopy analysis indicated that HLA-G protein expression in embryos was not homogeneous; mostly, it was confined to blastocysts localized on trophectoderm and trophectoderm projections. Single-particle fluorescent imaging analysis of HLA-G on the cell surface of JEG-3 cells showed that HLA-G particles were mostly monomeric, but dimeric and higher order oligomers were also observed. These results suggest that HLA-G play an important role in preimplantation embryo development. However, the observed expression of HLA-G in arrested and chromosomally abnormal embryos indicates that HLA-G testing should be used with caution and in conjunction with conventional methods of embryo screening and selection.

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Secretion of human leukocyte antigen-G by human embryos is associated with a higher in vitro fertilization pregnancy rate

Cited by 101 publications

References 32 publications

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome

Differential Expression of Alternatively Spliced Transcripts of HLA-G in Human Preimplantation Embryos and Inner Cell Masses

Analysis of HLA-G in Maternal Plasma, Follicular Fluid, and Preimplantation Embryos Reveal an Asymmetric Pattern of Expression

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