Secretion of Phenolic Compounds into Root Exudates of Pea Seedlings upon Inoculation with Rhizobium leguminosarum bv. viceae or Pseudomonas siringae pv. pisi

Makarova, L. E.; Дударева, Л. В.; Petrova, I. G.; Vasil’eva, G. G.

doi:10.1134/s0003683816020095

Cited by 15 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Naringenin (Fig. 1A), a flavonoid released by pea seedlings [11], had no effect at all [8]. However, a detailed understanding of the origins of this specificity is still lacking.…”

Section: Introductionmentioning

confidence: 99%

The induction mechanism of the flavonoid‐responsive regulator FrrA

et al. 2021

View full text Add to dashboard Cite

Bradyrhizobium diazoefficiens, a bacterial symbiont of soybean and other leguminous plants, enters a nodulation-promoting genetic programme in the presence of host-produced flavonoids and related signalling compounds. Here, we describe the crystal structure of an isoflavonoidresponsive regulator (FrrA) from Bradyrhizobium, as well as cocrystal structures with inducing and noninducing ligands (genistein and naringenin, respectively). The structures reveal a TetR-like fold whose DNAbinding domain is capable of adopting a range of orientations. A single molecule of either genistein or naringenin is asymmetrically bound in a central cavity of the FrrA homodimer, mainly via C-H contacts to the psystem of the ligands. Strikingly, however, the interaction does not provoke any conformational changes in the repressor. Both the flexible positioning of the DNA-binding domain and the absence of structural change upon ligand binding are corroborated by small-angle X-ray scattering (SAXS) experiments in solution. Together with a model of the promoter-bound state of FrrA our results suggest that inducers act as a wedge, preventing the DNA-binding domains from moving close enough together to interact with successive positions of the major groove of the palindromic operator.

show abstract

“…Naringenin (Fig. 1A), a flavonoid released by pea seedlings [11], had no effect at all [8]. However, a detailed understanding of the origins of this specificity is still lacking.…”

Section: Introductionmentioning

confidence: 99%

The induction mechanism of the flavonoid‐responsive regulator FrrA

et al. 2021

View full text Add to dashboard Cite

show abstract

“…viceae , Pseudomonas syringae pv. pisi and Plasmodiophora brassicae (Makarova et al, 2016). However, we did not detect significant accumulation of naringenin in tobacco after infection with P .…”

Section: Discussionmentioning

confidence: 99%

“…viceae , Pseudomonas syringae pv. pisi or Plasmodiophora brassicae (Makarova et al, 2016; Paesold et al, 2010). In addition, naringenin has shown anti‐inflammatory, antiviral and antifungal activities, for example against Fusarium spp.…”

Section: Introductionmentioning

confidence: 99%

Naringenin confers defence against Phytophthora nicotianae through antimicrobial activity and induction of pathogen resistance in tobacco

Sun

Wang

et al. 2022

Molecular Plant Pathology

View full text Add to dashboard Cite

Tobacco black shank caused by Phytophthora nicotianae is a serious disease in tobacco cultivation. We found that naringenin is a key factor that causes different sensitivity to P. nicotianae between resistant and susceptible tobacco. The level of basal flavonoids in resistant tobacco was distinct from that in susceptible tobacco. Of all flavonoids with different content, naringenin showed the best antimicrobial activity against mycelial growth and sporangia production of P. nicotianae in vitro. However, naringenin showed very low or no antimicrobial activity to other plant pathogens.We found that naringenin induced not only the accumulation of reactive oxygen species, but also the expression of salicylic acid biosynthesis-related genes. Naringenin induced the expression of the basal pathogen resistance gene PR1 and the SAR8.2 gene that contributes to plant resistance to P. nicotianae. We then interfered with the expression of the chalcone synthase (NtCHS) gene, the key gene of the naringenin synthesis pathway, to inhibit naringenin biosynthesis. NtCHS-RNAi rendered tobacco highly sensitive to P. nicotianae, but there was no change in susceptibility to another plant pathogen, Ralstonia solanacearum. Finally, exogenous application of naringenin on susceptible tobacco enhanced resistance to P. nicotianae and naringenin was very stable in this environment. Our findings revealed that naringenin plays a core role in the defence against P. nicotianae and expanded the possibilities for the application of plant secondary metabolites in the control of P. nicotianae.

show abstract

“…Pisi and X. campestris pv. Malvacearum ( Beckman, 2000 ; Kangatharalingam et al, 2002 ; Päsold et al, 2010 ; Makarova et al, 2016 ). Furthermore, we found that naringenin induces pathogen resistance to Pst DC3000 ( Figures 1A,B ), suggesting that accumulated naringenin by pathogen leads to pathogen resistance ( Jia et al, 2010 ; Yang et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…Naringenin is accumulated by infected biotrophic pathogen Plasmodiophora brassicae and Pseudomonas siringae pv. pisi ( Päsold et al, 2010 ; Makarova et al, 2016 ). Furthermore, naringenin is reported to confer not only anti-inflammatory and antiviral activities ( Hartogh and Tsiani, 2019 ) but also resistance against Fusarium ( Skadhauge et al, 1997 ) and Pyricularia oryzae ( Padmavati et al, 1997 ).…”

Section: Introductionmentioning

confidence: 99%

Naringenin Induces Pathogen Resistance Against Pseudomonas syringae Through the Activation of NPR1 in Arabidopsis

Kim

Bahk

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Flavonoids are well known for the coloration of plant organs to protect UV and ROS and to attract pollinators as well. Flavonoids also play roles in many aspects of physiological processes including pathogen resistance. However, the molecular mechanism to explain how flavonoids play roles in pathogen resistance was not extensively studied. In this study, we investigated how naringenin, the first intermediate molecule of the flavonoid biosynthesis, functions as an activator of pathogen resistances. The transcript levels of two pathogenesis-related (PR) genes were increased by the treatment with naringenin in Arabidopsis. Interestingly, we found that naringenin triggers the monomerization and nuclear translocation of non-expressor of pathogenesis-related genes 1 (NPR1) that is a transcriptional coactivator of PR gene expression. Naringenin can induce the accumulation of salicylic acid (SA) that is required for the monomerization of NPR1. Furthermore, naringenin activates MPK6 and MPK3 in ROS-dependent, but SA-independent manners. By using a MEK inhibitor, we showed that the activation of a MAPK cascade by naringenin is also required for the monomerization of NPR1. These results suggest that the pathogen resistance by naringenin is mediated by the MAPK- and SA-dependent activation of NPR1 in Arabidopsis.

show abstract

Secretion of Phenolic Compounds into Root Exudates of Pea Seedlings upon Inoculation with Rhizobium leguminosarum bv. viceae or Pseudomonas siringae pv. pisi

Cited by 15 publications

References 13 publications

The induction mechanism of the flavonoid‐responsive regulator FrrA

The induction mechanism of the flavonoid‐responsive regulator FrrA

Naringenin confers defence against Phytophthora nicotianae through antimicrobial activity and induction of pathogen resistance in tobacco

Naringenin Induces Pathogen Resistance Against Pseudomonas syringae Through the Activation of NPR1 in Arabidopsis

Contact Info

Product

Resources

About