Secretome analysis using a hollow fiber culture system for cancer biomarker discovery

“…Alternatively, the hollow fiber culture (HFC) system could be used to reduce cell death and detect low abundance proteins in the large volumes of collected CM [ 47 ]. This system, composed of small fibers sealed in a cartridge shell, provides an in vivo-like 3D environment able to hold many cells in a small volume, thereby concentrating the secreted proteins.…”

Section: Secretome Samplesmentioning

confidence: 99%

Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer

2020

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Searching for new cancer-related biomarkers is a key priority for the early detection of solid tumors, such as colorectal cancer (CRC), in clinically relevant biological fluids. The cell line and/or tumor tissue secretome represents a valuable resource for discovering novel protein markers secreted by cancer cells. The advantage of a secretome analysis is the reduction of the large dynamic range characterizing human plasma/serum, and the simultaneous enrichment of low abundance cancer-secreted proteins, thereby overcoming the technical limitations underlying the direct search in blood samples. In this review, we provided a comprehensive overview of recent studies on the CRC secretome for biomarker discovery, focusing both on methodological and technical aspects of secretome proteomic approaches and on biomarker-independent validation in CRC patient samples (blood and tissues). Secretome proteomics are mainly based on LC-MS/MS analyses for which secretome samples are either in-gel or in-solution trypsin-digested. Adequate numbers of biological and technical replicates are required to ensure high reproducibility and robustness of the secretome studies. Moreover, another major challenge is the accuracy of proteomic quantitative analysis performed by label-free or labeling methods. The analysis of differentially expressed proteins in the CRC secretome by using bioinformatic tools allowed the identification of potential biomarkers for early CRC detection. In this scenario, this review may help to follow-up the recent secretome studies in order to select promising circulating biomarkers to be validated in larger screenings, thereby contributing toward a complete translation in clinical practice.

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“…For example, in Chiu et al, a cHFB in the configuration shown in Figure 3C was used for secretome analysis and cancer biomarker discovery. The authors reported several advantages of using cHFBs in this field, including a high expansion and lower cell lysis rate and, consequently, an increased volume of samples to be analyzed when compared to static culture conditions (64) (Tab. I).…”

Section: Other Applications Of Hfbsmentioning

confidence: 99%

Hollow Fiber Bioreactor Technology for Tissue Engineering Applications

et al. 2016

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Hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological vascular networks and engineer organs and tissues in vitro. The reason for this lies in the interesting features of this bioreactor type, including excellent mass transport properties. Indeed, hollow fiber bioreactors allow limitations to be overcome in nutrient transport by diffusion, which is often an obstacle to engineer sizable constructs in vitro. This work reviews the existing literature relevant to hollow fiber bioreactors in organ and tissue engineering applications. To this purpose, we first classify the hollow fiber bioreactors into 2 categories: cylindrical and rectangular. For each category, we summarize their main applications both at the tissue and at the organ level, focusing on experimental models and computational studies as predictive tools for designing innovative, dynamic culture systems. Finally, we discuss future perspectives on hollow fiber bioreactors as in vitro models for tissue and organ engineering applications.

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Secretome analysis using a hollow fiber culture system for cancer biomarker discovery

Cited by 7 publications

References 76 publications

Tumor-dependent secretion of close homolog of L1 results in elevation of its circulating level in mouse model for human lung tumor

Tumor-dependent secretion of close homolog of L1 results in elevation of its circulating level in mouse model for human lung tumor

Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer

Hollow Fiber Bioreactor Technology for Tissue Engineering Applications

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