Secretory phospholipase A2 activity in blood serum: The challenge to sense

Алексеева, А. С.; Коротаева, А. А.; Самойлова, Е. В.; Volynsky, Pavel E.; Водовозова, Е. Л.; Болдырев, Иван

doi:10.1016/j.bbrc.2014.10.069

Cited by 11 publications

(9 citation statements)

References 17 publications

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“…Hence, BODIPY-Sudan III-PC probe 33 (Scheme 6) was used to measure the sPLA 2 activity directly in blood serum. 12 The approach developed circumvents the required probe purification common to chromatography based methods which can result in a loss of accuracy. These studies were based on the FRET phenomena, although in this case, the occurrence of fluorescence can also be due to the possible bond of the probe to proteins in serum.…”

Section: Glycerophospholipid Conjugatesmentioning

confidence: 99%

“…This correlation suggests that sPLA 2 can be potentially used as a biomarker of cardiovascular risk. Hence, BODIPY-Sudan III-PC probe 33 (Scheme ) was used to measure the sPLA 2 activity directly in blood serum . The approach developed circumvents the required probe purification common to chromatography based methods which can result in a loss of accuracy.…”

Section: Applications Of Fluorescent Glycerophospholipid Conjugatesmentioning

confidence: 99%

“…Different synthetic strategies were developed for the synthesis of both probe 27b and 27c (Scheme 5). 16 Alekseeva et al 12 developed a new fluorescent lipid probe encapsulated in vesicles to monitor sPLA 2 activity directly in blood serum. The probe contained a Sudan III quencher at the end of a 10-carbon-atom chain at the sn-2 position and a fluorescent BODIPY reporter group attached to the end of sn-1 fatty acid with a longer carbon chain (C 13 ).…”

Section: ■ Introductionmentioning

confidence: 99%

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Emergent Glycerophospholipid Fluorescent Probes: Synthesis and Applications

et al. 2019

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Fluorescent labeling through bioconjugation is the preferred tool for the investigation of biological functions involving lipids, namely for clarifying metabolic pathways and molecular mechanisms of diseases. The lack of functionalized lipid probes with biological and physicochemical properties suitable for these studies is still a major limitation. Moreover, the synthesis of these probes is challenging and strongly dependent on the application envisioned. The objective of this Review is to highlight advances in the application of fluorescent glycerophospholipid probes through innovative approaches in the synthesis reported in the past decade. The reaction pathways, choice of fluorophore, and location of fluorophore in the glycerophospholipid structure are critically addressed. The relevance of these bioconjugates is exemplified with applications using advanced analysis by fluorescence enhancement or quenching to unravel biomembrane structure features and phospholipase activity. Finally, this Review reinforces the need for innovative and more efficient routes for the synthesis of tailored glycerophospholipids fluorescent conjugates.

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Section: Glycerophospholipid Conjugatesmentioning

confidence: 99%

Section: Applications Of Fluorescent Glycerophospholipid Conjugatesmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Emergent Glycerophospholipid Fluorescent Probes: Synthesis and Applications

et al. 2019

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“…Thus, the activity and selectivity of sPLA2 is determined by the joint operation of IBS and the catalytic site. A wide range of techniques has been developed to detect the final hydrolytic activity of sPLA2 [4,5], but for a more detailed study of the functioning of inter-Abbreviations: sPLA2, secretory phospholipase A2; bvPLA2, phospholipase A2 from Apis mellifera bee venom; IBS, interfacial binding site; POPC, palmitoyloleoylphosphatidylcholine; POPG, palmitoyloleoylphosphatidylglycerol; TMB-PC, 1,3,5,7tetramethyl-BODIPY-labeled phosphatidylcholine.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the Phospholipase A2 Selectivity on POPC/POPG Membranes Using the Interaction Map

Алексеева

Volynsky

Болдырев

2021

Biochem. Moscow Suppl. Ser. A

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The regulation of the activity and selectivity of phospholipase A2 (PLA2), which is capable of cleaving fatty acid in the second position (sn-2) of the phospholipid, is carried out through the membrane-binding and catalytic sites of the enzyme. For hydrolytic activity, PLA2 must first bind to the phospholipid membrane, and the binding efficiency depends on the composition of the membrane. The membrane-binding site of PLA2 is formed by several tens of amino acids and its composition differs from enzyme to enzyme; hydrophobic and positively charged amino acids play a key role in the interaction. In this work, we investigated the interaction of PLA2 from bee venom with phospholipid bilayers of palmitoyl oleoylphosphatidylcholine (POPC) containing different amounts of palmitoyloleoylphosphatidylglycerol (POPG). On the basis of the measurements of the protein intrinsic fluorescence and the anisotropy of the fluorescence of the lipid probe we propose the construction of lipid–protein interaction maps, which reflect both the efficiency of protein binding and changes in the structure of the membrane. These changes cause alterations in the fluorescence anisotropy of the label, which in turn is a measure of the mobility of the lipid environment of the fluorescent probe. Analysis of interaction maps showed that there is a relationship between lipid mobility and enzyme binding efficiency: the optimum interaction of PLA2 with membranes from a POPC/POPG mixture lies in the region of the highest lipid mobility, and not in the region of the highest negative charge. This dependence complements the existing understanding of the process of recognition of the membrane surface by the enzyme and the selection of lipids by the enzyme already bound to the membrane. The proposed mapping method can be extended to other membrane-active proteins.

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“…In the course of our development of membrane-bound probes and specific quenchers of their fluorescence [11,12], we have faced the necessity of designing a new membrane-bound black quencher specific to pyrene fluorescence-a quencher for pyrene and pyrene-based lipid probes. Here we report the synthesis of this black quencher.…”

Section: Introductionmentioning

confidence: 99%

3-{4-[(E)-{4-[(E)-Phenyldiazenyl]phenyl}diazenyl]phenoxy}propane-1,2-diol

Chupin

Болдырев

2017

Molbank

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Abstract:Title compound was designed to be a black quencher of pyrene fluorescence. It was made amphiphilic to serve as a membrane-bound probe. The synthesis is a two-step procedure. The first step is a Mitsunobu reaction of [{(phenyldiazenyl)phenyl}diazenyl]phenol with 1,2-O-isopropylideneglycerol. The second step is the cleavage of the isopropylidene protecting group. The title compound has the extinction coefficient 59,000 at λmax = 380 nm. The Forster distance between the title compound and the pyrene was found to be 37.8 Å.

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Secretory phospholipase A2 activity in blood serum: The challenge to sense

Cited by 11 publications

References 17 publications

Emergent Glycerophospholipid Fluorescent Probes: Synthesis and Applications

Emergent Glycerophospholipid Fluorescent Probes: Synthesis and Applications

Estimation of the Phospholipase A2 Selectivity on POPC/POPG Membranes Using the Interaction Map

3-{4-[(E)-{4-[(E)-Phenyldiazenyl]phenyl}diazenyl]phenoxy}propane-1,2-diol

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