Secukinumab - a stupendous option in psoriasis management

Sriram, Damal Kandadai; George, Melvin; Snehalatha, K.; Gowri, Govindaraj

doi:10.18203/2320-6012.ijrms20162897

Cited by 1 publication

(2 citation statements)

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“…The retention factor values also depended on the solvent where the samples were dissolved either methanol or dichloromethane. From the results of paper [16], the Rf value of the green tea is 0.55, this result is obtained when is used the mobile phase of ethyl acetate: acetic acid (19:1, v/v) with the distance travelled by the solvent and by the solute 4.5 cm and 2.5 cm.…”

Section: Resultsmentioning

confidence: 91%

“…According to the results from the scientific paper [16] the content of caffeine in "Darjeeling Green tea" is about 2.92 % while in the scientific paper [17] the yield of caffeine in the green tea is 0.65 % which are slightly different from the results that we have obtained in our paper work, the yield of caffeine is 2.04 % in the green tea from the grocery store for health nutrition and in the tea from the green market is 0.06 %. The results obtained from Ashihara and Kubota (1986) found that caffeine biosynthesis is the most active in young tea leaves and buds [18].…”

Section: Resultsmentioning

confidence: 99%

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Development of thin-layer chromatographic method for determination of caffeine in black, green, and white tea

2021

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Caffeine is naturally present in tea and coffee giving the pleasant and stimulant effect. Several different types of teas, black, green, and white teas bought in market were analysis for caffeine content. The boiled sample tea was filtered through filter paper. Lead(II) acetate was used to separate tannins from caffeine followed by filtration through filter paper with a black ribbon. The liquid-liquid extraction was carried out using dichloromethane (3×5 mL) and sodium sulfate as a drying agent. The TLC method was performed on Merck precoated silica gel plates 5×10 cm (60F254, 200 μm) using either methanol or dichloromethane as solvents and the mobile phases were glacial acetic acid and ethyl acetate (95:5, v/v), while the second one was consisted of ethyl acetate and ethanol (80:20, v/v), respectfully. The Rf values were 0.36 and 0.86 for the first and the second mobile phase, respectively, in comparison to the standard caffeine. The values for pH of boiled sample teas were in the range from 4.85 to 5.80. The most abundant tea sample for caffeine was determined in green tea bought in the grocery store for health nutrition (2.04 %). The yield for tea samples from green market, white tea and two tea black samples were 0.06, 0.71, 0.07, and 0.05%, respectively. The developed TLC method can be used for determination of caffeine content in tea samples.

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Section: Resultsmentioning

confidence: 91%