Securing the aorta for organ perfusion in donation after cardiac death donors; novel techniques and a cautionary tale

Ray, Christopher; Kanwar, Aditya; Noormohamed, Mohamed Saleem; Sohrabi, Soroush; Ray, S.; Navarro, Alex; Talbot, David

doi:10.1111/j.1432-2277.2009.00873.x

Cited by 2 publications

(1 citation statement)

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“…However, one major limitation to clinical islet transplantation is the availability of donor organs. Donation after cardiac death (DCD) (Ray et al, 2009), defined as the irreversible cessation of circulatory and respiratory functions, has become an internationally recognized organ source.…”

Section: Introductionmentioning

confidence: 99%

Influence of adenosine on preservation of porcine pancreas in islet transplantation

Song¹,

Dz²,

Cheng³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Ischemia time during transplantation has greatly restricted the quality and utilization of grafts. To improve the quality of islet transplantation, adenosine was added into the University of Wisconsin (UW) pancreas perfusate to assess its effect on islet yield and function in porcine pancreas. Ten pancreata from donation after cardiac death pigs were obtained and randomly divided into two groups: control group (N = 5) with UW perfusion solution, and experimental group (N = 5) with adenosine-enriched UW perfusion solution. The yield and purity of the islet cells were counted after they were collected, purified, and stained with dithizone. Acridine orange/propidium iodide staining was applied to determine islet cell viability. Islet function was assessed by glucose stimulated insulin secretion assays, and released insulin was quantified by enzyme-linked immunosorbent assay. The metabolic substrates of the pancreas were analyzed by trace dialysis technology. We found that the addition of adenosine in UW perfusion solution significantly increased the yield, purity and viability of islet cells, as well as enhanced their insulin release. In addition, the levels of metabolic substrates, pyruvate and lactate, were significantly reduced. The addition of adenosine could effectively increase islet cell viability during mechanical perfusions, which may improve islet transplantation. This perfusion protocol may be clinically feasible, and should be considered in the clinical setting.

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Section: Introductionmentioning

confidence: 99%