Seed conservation in ex situ genebanks—genetic studies on longevity in barley

Nagel, Manuela; Vogel, Hermine; Landjeva, S.; Buck-Sorlin, Gerhard; Lohwasser, U.; Scholz, Uwe; Börner, Andreas

doi:10.1007/s10681-009-9975-7

Cited by 76 publications

(71 citation statements)

References 24 publications

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“…The only lot with a significantly lower longevity was DT6, but this difference could be explained by its initial germination percentage, also considerably lower (77%) than in the remaining lots (above 92%), which indicates that DT6 had already deteriorated at the start of the experiment. While seed longevity has been found to be relatively similar within some genera and families (Walters et al, 2005;Probert et al, 2009), there are previous reports of highly variable seed longevity even among related species (Walters et al, 2005;Pérez-García et al, 2009;van Treuren et al, 2013), lots of the same cultivar (Nagel et al, 2009;Niedzielski et al, 2009;Nagel and Borner, 2010), or wild seed lots (Kochanek et al, 2009). Within Brassicaceae, longevity was found to vary greatly among wild species stored both at high and low humidity (Mira et al, 2014).…”

Section: Discussionmentioning

confidence: 97%

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Lazar¹,

Mira

Pamfil³

et al. 2014

Turk J Agric For

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The aim of this study was to determine whether the loss of germinability and vigour in wall-rocket seed lots was related to electrolyte leakage. Ten seed lots of 2 wall-rocket species (9 of Diplotaxis tenuifolia and one of D. erucoides (Brassicaceae)) were stored at 45 °C and 70% relative humidity and electrolyte leakage was measured using the conductivity test. Small differences in longevity were found among species or seed lots, suggesting that a similar relative longevity might be expected within the genus Diplotaxis after artificial ageing, and that it will be above the average of that found in other species of the same family at similar storage conditions. Electrolyte loss was strongly related to loss of seed viability. There was a negative linear relationship between conductivity and seed germination (R 2 = 0.85), and a positive relationship with T 50 (R 2 = 0.77). Small differences were found among species or seed lots regarding the correlation between electrolyte leakage and seed germination and vigour. Our results indicate that the conductivity method is able to predict the viability of wall-rocket seeds stored at 45 °C and 70% RH, with the same regression equation of conductivity and germination (-33.97x + 135.59) being able to predict the viability of different seed lots and species. A conductivity level ranging from 1.4 to 2.2 mS g -1 DW would indicate that the potential of a Diplotaxis seed lot to germinate has decreased to 80%. The correlation between electrolyte leakage and seed germination provides a valuable means for early detection of seed viability in wall-rocket seeds.

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Section: Discussionmentioning

confidence: 97%

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Lazar¹,

Mira

Pamfil³

et al. 2014

Turk J Agric For

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“…Seed longevity is determined by a number of environmental and genetic factors, and while many attempts have been made to optimize storage conditions (Ellis et al 1982(Ellis et al , 1993Barzali et al 2005), its inheritance and genetic control is poorly researched. Both inter-and intraspecific variability have been demonstrated in model and crop plant species (Walters et al 2005), and genes responsible for differences in seed longevity have been identified in A. thaliana (Clerkx et al 2004), cabbage (Bettey et al 2000), rice (Miura et al 2002;Sasaki et al 2005;Zeng et al 2006;Xue et al 2008), and barley (Nagel et al 2009). In rice, a series of mapping populations revealed QTL for seed longevity/storability mapping to seven chromosomes; however, only those on chromosome 9 were consistently identified.…”

Section: Qtl For Seed Longevitymentioning

confidence: 99%

“…(Bettey et al 2000), rice (Cui et al 2002), barley (Edney and Mather 2004), sunflower (Al Chaarani et al 2005) and wheat (Landjeva et al 2008). Genomic regions harbouring QTL associated with seed longevity have been identified in rice (Miura et al 2002;Sasaki et al 2005;Zeng et al 2006;Xue et al 2008), Arabidopsis thaliana (Clerkx et al 2004) and barley (Nagel et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Genetic mapping within the wheat D genome reveals QTL for germination, seed vigour and longevity, and early seedling growth

2009

Self Cite

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Quantitative trait loci (QTL) controlling germination, seed vigour and longevity, and early seedling growth were identified using a set of common wheat lines carrying known D genome introgression segments. Seed germination (capacity, timing, rate and synchronicity) was characterized by a standard germination test, based either on the 1 mm root protrusion (germination sensu stricto) or the development of normal seedlings. To quantify seed vigour, the same traits were measured from batches of seed exposed for 72 h at 43°C and high (ca. 100%) humidity. Seed longevity was evaluated from the relative trait values. Seedling growth was assessed both under non-stressed and under osmotic stress conditions. Twenty QTL were mapped to chromosomes 1D, 2D, 4D, 5D, and 7D. Most of the QTL for germination sensu stricto clustered on chromosome 1DS in the region Xgwm1291-Xgwm337. A region on chromosome 7DS associated with Xgwm1002 harboured loci controlling the development of normal seedlings. Seed vigour-related QTL were present in a region of chromosome 5DL linked to Xgwm960. QTL for seed longevity were coincident with those for germination or seed vigour on chromosomes 1D or 5D. QTL for seedling growth were identified on chromosomes 4D and 5D. A candidate homologues search suggested the putative functions of the genes within the respective regions. These results offer perspectives for the selection of favourable alleles to improve certain vigour traits in wheat, although the negative effects of the same chromosome regions on other traits may limit their practical use.

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“…In sunflower seeds, reductions in seed viability and vigor that occur during aging are associated with accumulation of hydrogen peroxide, accelerated lipid peroxidation, and reduced activity (Kibinza et al, 2006;Nagel et al, 2009;Li et al, 2015). Metallothioneins (MTs) serve as signaling molecules and ROS scavengers, both within and outside the nucleus, , which highlights their potential interaction with DNA repair machinery .…”

Section: Reactive Oxygen Species (Ros) and Signalingmentioning

confidence: 99%

“…Quantitative trait loci associated with seed longevity have been thoroughly investigated, in order to elucidate the genetic basis of seed deterioration (Shatters et al, 1995;Arif et al, 2012). As a result of these efforts, genes that govern seed longevity have been identified and may facilitate the prediction of the longevity in plant germplasm repertoires (Nagel et al, 2009;Vijay et al, 2009). For example, four genomic regions associated with wheat seed longevity are known to include genes related to stress responses and spike traits (Arif et al, 2012).…”

Section: Molecular Markersmentioning

confidence: 99%

Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and ex-situ Conservation

Ahmad¹,

Shah²,

Rehman³

et al. 2017

Current Issues in Molecular Biology

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Abbreviations:AFLP, amplified fragment length polymorphism AGO, argonaute miRNA, micro RNA NGS, next generation sequencing PCD, programmed cell death PIMT, protein L-isoaspartyl methyltransferase PRC2, Polycomb repressive complex 2 RAPD, random amplification of polymorphic DNA ROS, reactive oxygen species SSR, simple sequence repeat TUNEL, terminal deoxynucleotide transferasemediated dUTP nick-end labeling qRT-PCR, quantitative reverse transcriptional polymerase chain reaction Abstract For human food security, the preservation of 7.4 million ex-situ germplasm is a global priority. However, ex situ-conserved seeds are subject to aging, which reduces their viability and ultimately results in the loss of valuable genetic material over long periods. Recent progress in seed biology and genomics has revealed new opportunities to improve the long-term storage of ex-situ seed germplasm. This review summarizes the recent improvements in seed physiology and genomics, with the intention of developing genomic tools for evaluating seed aging. Several lines of seed biology research have shown promise in retrieving viability signal from various stages of seed germination. We conclude that seed aging is associated with mitochondrial alteration and programmed cell death, DNA and enzyme repair, anti-oxidative genes, telomere length, and epigenetic regulation. Clearly, opportunities exist for observing seed aging for developing genomic tools to increment the traditional germination test for effective conservation of ex-situ germplasm.

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Seed conservation in ex situ genebanks—genetic studies on longevity in barley

Cited by 76 publications

References 24 publications

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Genetic mapping within the wheat D genome reveals QTL for germination, seed vigour and longevity, and early seedling growth

Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and ex-situ Conservation

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