Seed quality in hybrid swarm populations of Pinus mugo Turra and P. sylvestris L.

Maňka, Peter; Vooková, B.; Salaj, Terézia; Čamek, Vladimír; Boleček, Peter; Gömöry, Dušan

doi:10.1007/s00606-008-0121-0

Cited by 5 publications

(12 citation statements)

References 20 publications

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“…In some cases, this can be improved by using the stored pollen from the preceding year (Lanteri et al 1993). Also, pollen germination can be important for obtaining interspecific and intraspecific hybrids, which can exhibit reduced pollen germinability (Kormutak et al 2007). …”

Section: Discussionmentioning

confidence: 99%

“…The collected pollen was dried during 48 h in the thermostat at 30°C, and purified by passing through a series of sieves, up to 0.2 mm. A part of the fresh pollen was immediately tested for germination, and the other part was stored in the sealed glass vials and in a desiccator over Silica Gel at 4 different storage temperatures: 1) room temperature: 23 ±1°C, 2) +4°C, 3) -15°C and 4) -20°C (Lanteri et al 1993, Kormutak et al 2007). …”

Section: Collection and Storage Of Pollenmentioning

confidence: 99%

“…Most studies of variability morpho-physiological traits related to pollen of species level, but only a few authors dealt with individual variability (Kormutak et al 1994(Kormutak et al , 2007Nikolić 1996;Slobodnik and Krizo 1997;Nikolić and Tošić 2006). Reports about individual variability of Serbian spruce flowering and pollen germination are still rare and insufficient (Grbović 1998;Grbović and Isajev 1997;Šijacić-Nikolić and Isajev 2001;Batos and Nikolić 2004).…”

Section: Introductionmentioning

confidence: 99%

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Variability of in vitro germination of Picea omorika pollen

Batos¹,

Nikolić²

2012

Dendrobiology

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Serbian spruce (Picea omorika /Panc./Purkyne) is a Balkan endemic and Tertiary relict, therefore it has an exceptional significance from the historical-geographical aspect as well as from the aspect of tree improvement and biodiversity conservation. Pollen was collected from 24 trees in two consecutive years and analyzed once a month. Germination of fresh pollen and pollen stored under different temperatures (room temperature: 23 ±1°C, +4°C, -15°C and -20°C) by in vitro method on a medium with 10% sucrose was analyzed. Fresh pollen germination was 53.63% and 68.06% for pollen collected in first and second year, respectively. After one year of storage at -20°C pollen germination was 22.36% and 60.72%, respectively. The differences between pollen collection years, temperature treatments, storage periods and individuals were statistically significant. Since pollen germination rates of individual trees between the first and the second year showed weak positive correlation, one could conclude that germination of fresh pollen grains is under the certain influence of environmental conditions. It is determined strong positive correlations between fresh and stored pollen at -20°C from both years of pollen collection.

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Section: Discussionmentioning

confidence: 99%

Section: Collection and Storage Of Pollenmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Variability of in vitro germination of Picea omorika pollen

Batos¹,

Nikolić²

2012

Dendrobiology

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“…The attempt has therefore been made to analyze histologically the seed development in these hybrids on the locality Habovka, northern Slovakia, aiming in shedding some light on this aspect of their fertilization process and embryogeny. The proof supporting hybrid nature of the pine population at the respective locality is based on needle and seed characters (Musil 1977a, b;Viewegh 1981;Staszkiewicz 1994) as well as on molecular evidence involving cpDNA and mtDNA data (Kormutak et al 2007a, b).…”

Section: Introductionmentioning

confidence: 99%

Abortive embryogenesis in hybrid swarm populations of Pinus sylvestris L. and Pinus muga Turra

Vooková

Maňka

Salaj

et al. 2008

Trees

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Comparative study on fertilization process in Pinus sylvestris, Pinus mugo and in their putative hybrid swarm individuals was done involving pre-zygotic and post-zygotic stages. The amount of surviving ovules from open pollination reflecting the mode of interaction between pollen grains and nucellar tissue of an ovule averaged at 8.1 of sound ovules per conelet in Pinus sylvestris, 7.3 ovules in the hybrid swarm population and at 4.9 ovules in Pinus mugo. A strong correlation was observed between the number of surviving ovules and the proportion of germinating seeds in the compared species and hybrids. Normal course of embryogenesis in Pinus sylvestris and Pinus mugo contrasted with increased frequency of disturbances observed in the hybrid swarm individuals. The differential survival rates of the ovules and deviations from typical pattern of embryogenesis are discussed from the standpoint of cross-ability relationship between Pinus sylvestris and Pinus mugo.

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“…To date studies conducted on Pinus mugo have concerned mostly its anatomical and morphological variability (Boratyńska et al 2004;, allozyme variation (Prus-G lowacki & Szweykowski 1983;Slavov & Zhelev 2004), clonal struc-622 K. Celiński et al Table 1. (Wachowiak et al 2006;Wachowiak et al 2008;Kormutak et al 2008;Kormutak et al 2009). However, up to date no data have been available on the genetic diversity of Pinus mugo populations using nuclear microsatellite markers.…”

Section: Introductionmentioning

confidence: 99%

Cross-species amplification and characterization of microsatellite loci in Pinus mugo Turra

et al. 2013

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Pinus mugo (dwarf mountain pine) is an important component of European mountain ecosystems. However, little is known about the present genetic structure and population differentiation of this species at the DNA level, possibly due to a lack of nuclear microsatellite markers (SSR) developed for Pinus mugo. Therefore in this study we transferred microsatellite markers originally developed for Pinus sylvestris and Pinus taeda to Pinus mugo. This cross-species amplification approach is much faster and less expensive than isolation and characterization of new microsatellite markers. The transfer rates from the source species to Pinus mugo were moderately low (26%). There were no differences in microsatellite repeat motifs between the source species and Pinus mugo. Nuclear microsatellite markers successfully transferred to Pinus mugo can be applied to various genetic studies on this species, due to the high level of their polymorphism and high value of polymorphic information content.

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Seed quality in hybrid swarm populations of Pinus mugo Turra and P. sylvestris L.

Cited by 5 publications

References 20 publications

Variability of in vitro germination of Picea omorika pollen

Variability of in vitro germination of Picea omorika pollen

Abortive embryogenesis in hybrid swarm populations of Pinus sylvestris L. and Pinus muga Turra

Cross-species amplification and characterization of microsatellite loci in Pinus mugo Turra

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