Abbreviations : LAI, leaf area index; SPAD, chlorophyll meter; 0-0N, 0 and 0 g N m -2 applied at active tillering and jointing, respectively; 4-2N, 4 and 2 g N m -2 applied at active tillering and jointing, respectively Abstract : We examined the effects of seeding rate, 50 or 150 seeds m -2 , nitrogen (N) application rate at active tillering and jointing, 4 and 2 g N m -2 , respectively, or none, and N application rate at anthesis, 0, 2, 4, or 6 g N m -2 , on grain yield and protein content of a bread wheat cultivar, 'Minaminokaori', during the 2004-2005 crop season in southwestern Japan. Grain yield was similar at a seeding rate of 50 and 150 seeds m -2 . It was higher when 4 and 2 g N m -2 were applied at active tillering and jointing, respectively (4-2N), than when no N was applied at these stages (0-0N). However, it was not infl uenced by N application rate at anthesis. Grain protein content was similar at 50 and 150 seeds m -2 . It was higher in 4-2N than in 0-0N. It was the highest when 6 g N m -2 was applied at anthesis, followed by 4, 2, and 0 g N m -2 . The SPAD value at anthesis was higher at 50 than 150 seeds m -2 , but leaf area index (LAI) at anthesis was similar at 50 and 150 seeds m -2 and protein content of grain was nearly the same at 50 and 150 seeds m -2 irrespective of N application rate at anthesis. LAI and the SPAD value were higher in 4-2N than in 0-0N and the protein content of grain was also higher in 4-2N than in 0-0N irrespective of N application rate at anthesis. Therefore, both LAI and the SPAD value may be important traits related to the N application rate at anthesis suitable for yielding wheat grain with a high protein content.