Segmentation pattern of zebrafish caudal fin is affected by developmental temperature and defined by multiple fusions between segments

Abstract

Caudal-fin lepidotrichia is composed of numerous segments, which are linked to each other by intersegmental joints. During fish growth, lepidotrichia elongate by the addition of new segments at their distal margin, whereas the length of each segment remains constant after it is formed. In the present paper, we examined whether the water temperature affects the segmentation pattern of the juvenile and adult caudal fin. For this purpose, zebrafish (Danio rerio) embryos and larvae were exposed to three different temperature conditions (24°C, 28°C, and 32°C) from the pharyngula stage (1 day postfertilization [dpf]) to metamorphosis, whereas the control temperature (28°C) was applied to all the groups before and after this period. Results demonstrated that water temperature had a significant effect on the length of the segments of each lepidotrichium, at both the juvenile and adult stages. Moreover, at higher temperatures, there was a significant proximal shift of the position of the first bifurcation of the second lepidotrichium of the dorsal lobe. At all the experimental conditions, the length of proximal segment was not constant during fish growth, but it followed a discontinuous saltatory growth. Histological analysis of the proximal lepidotrichia segments revealed that the observed apparent growth of segments is the result of fusions between segments. Fusion occurs not by mineralization of the intersegmental joints, but by bone deposition around the joints.

A Systematic Analysis of Metal and Metalloid Concentrations in Eight Zebrafish Recirculating Water Systems

“…13,14 Conversely, high concentrations can also lead to severe symptoms and disorders in reproduction, growth and development, 13,[15][16][17] metabolism, 10,18,19 and behavior. [20][21][22] The toxicity of particular metals is typically defined by their concentration of free ions in water, which is highly dependent on environmental parameters such as water hardness, conductivity, temperature, [23][24][25][26] and pH. 27,28 Environmental agencies such as the Environmental Protection Agency (EPA, USA) and the Australia, New Zealand Environment, and Conservation Council (ANZECC) developed guidelines for freshwater and saltwater levels for various metals (Table 1).…”

Exercise‐induced lordosis in zebrafish Danio rerio (Hamilton, 1822)

“…Anatomy and development of the skeleton in zebrafish are well characterized (Bensimon‐Brito et al ., 2012; Bird & Mabee, 2003; Christou et al ., 2018; Ferreri et al ., 2000; Parichy et al ., 2009). Today zebrafish is considered as a vertebrate model for human bone diseases ( e.g ., Clement et al ., 2008; Gistelinck et al ., 2016; Gistelinck et al ., 2018; Mackay et al ., 2013; Witten et al ., 2017).…”

More Bone with Less Minerals? The Effects of Dietary Phosphorus on the Post-Cranial Skeleton in Zebrafish

“…This study examines the post-cranial skeleton in zebrafish, given that the vertebral column is the most studied anatomical structure in biomedical research and in aquaculture [ 36 , 37 ]. Likewise, fin rays are popular for the study of bone regeneration and fish health [ 38 , 39 ]. Focusing on both endoskeletal and dermal skeletal elements, this study shows in detail how structure and mineralisation of different bone structures are affected by dietary P content.…”