Trichoderma fungi are considered relevant plant growth promoters for increasing the efficiency in the use of nutrients, as well as acting as biological control agents. This study aimed to select Trichoderma spp. isolates with potential for phosphate solubilization and their application as growth promoters in interaction with homeophatic preparations, in cherry tomato. Among 16 Trichoderma spp. isolates obtained from soils of organic tomato growing areas tested in vitro, together with a commercial product (Trichodermil®), two of them showed the ability for indole-3-acetic acid production and phosphate solubilization. The Trichoderma “R” had the highest mycelial growth speed index and presented twice as much spores than the commercial product. An in vivo experiment was also conducted in a greenhouse, in order to observe the potential of Trichoderma spp. isolates and homeophatic preparations on the cherry tomato growth promotion, using a randomized block experimental design, in a 4 x 3 factorial arrangement, with three Trichoderma isolates and two homeopathic preparations (Phosphorus 6CH and Carbo vegetabilis 6CH) + treatment without homeopathic preparation. The leaf area and dry mass of leaves and roots were determined. It was possible to observe that the isolate “R”, identified as Trichoderma asperellum, was effective in the cherry tomato growth promotion, while the homeopathic preparations applied did not show any effect.