Selected Nanomaterials’ Application Enhanced with the Use of Stem Cells in Acceleration of Alveolar Bone Regeneration during Augmentation Process

Zakrzewski, Wojciech; Rybak, Zbigniew; Szymonowicz, Maria; Wiglusz, Rafał J.

doi:10.3390/nano10061216

Cited by 38 publications

(27 citation statements)

References 205 publications

(233 reference statements)

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“…In this sense, recent advances have been made with gold nanoparticles as a biomaterial in dentistry due to their antifungal and antibacterial activity, mechanical properties and availability of different sizes and concentrations [ 14 ]. However, the studies focused on the use of bioactive nanoparticles for periodontal bone augmentation are very scarce, and most of them have been carried out with hydroxyapatite nanoparticles [ 15 , 16 ]. In this context, the recent developments in the preparation of mesoporous bioactive glass nanoparticles could provide a very interesting alternative for this purpose [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment

et al. 2020

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The incorporation and effects of hollow mesoporous nanospheres in the system SiO2–CaO (nanoMBGs) containing ipriflavone (IP), a synthetic isoflavone that prevents osteoporosis, were evaluated. Due to their superior porosity and capability to host drugs, these nanoparticles are designed as a potential alternative to conventional bioactive glasses for the treatment of periodontal defects. To identify the endocytic mechanisms by which these nanospheres are incorporated within the MC3T3-E1 cells, five inhibitors (cytochalasin B, cytochalasin D, chlorpromazine, genistein and wortmannin) were used before the addition of these nanoparticles labeled with fluorescein isothiocyanate (FITC–nanoMBGs). The results indicate that nanoMBGs enter the pre-osteoblasts mainly through clathrin-dependent mechanisms and in a lower proportion by macropinocytosis. The present study evidences the active incorporation of nanoMBG–IPs by MC3T3-E1 osteoprogenitor cells that stimulate their differentiation into mature osteoblast phenotype with increased alkaline phosphatase activity. The final aim of this study is to demonstrate the biocompatibility and osteogenic behavior of IP-loaded bioactive nanoparticles to be used for periodontal augmentation purposes and to shed light on internalization mechanisms that determine the incorporation of these nanoparticles into the cells.

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Section: Introductionmentioning

confidence: 99%

Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment

et al. 2020

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“…Numerous biomaterials, such as autologous bone, allografts, xenografts, alloplastic materials (i.e., nanomaterials), biologic mediators, and stem cells, have been successfully applied in various techniques to restore the horizontal and vertical dimensions of the alveolar ridges prior to or at the time of dental implant placement [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

A Modified Ridge Splitting Technique Using Autogenous Bone Blocks—A Case Series

et al. 2020

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Background: Alveolar atrophy following tooth loss is a common limitation of rehabilitation with dental implant born prostheses. Ridge splitting is a well-documented surgical method to restore the width of the alveolar ridge prior to implant placement. The aim of this case series is to present a novel approach to ridge expansion using only autogenous bone blocks. Methods: Patients with Kennedy Class I. and II. mandibles with insufficient bone width were included in this study. Ridge splitting was carried out with the use of a piezoelectric surgery device by preparing osteotomies and after mobilization of the buccal cortical by placing an autologous bone block harvested from the retromolar region as a spacer between the buccal and lingual cortical plates. Block-grafts were stabilized by osteosynthesis screws. Implant placement was carried out after a 3-month healing period. A total of 13 implants were placed in seven augmented sites of six patients. Results: Upon re-entry, all sites healed uneventfully. Mean ridge width gain was 2.86 mm, range: 2.0–5.0 mm. Conclusions: Clinical results of our study show that the modified ridge splitting technique is a safe and predictable method to restore width of the alveolar ridge prior to implant placement.

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“…Especially, in term of its osteoinductive capacity, the nano- and microtopography of a bone substitute material might be of great interest in order to mimic the unique nano- and microtopography of natural bone as closely as possible. In this regard, changes in the nanotopography of artificial surfaces were reported to directly regulate differentiation of stem cells [ 59 , 60 , 61 , 62 , 63 , 64 ]. Accordingly, we were able to show the potential of a defined nanotopography of about 30 nm pores present on the surface of collagen to induce osteogenic differentiation of human inferior turbinate as well as human mesenchymal stem cells in our previous work [ 12 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

Spongostan™ Leads to Increased Regeneration of a Rat Calvarial Critical Size Defect Compared to NanoBone® and Actifuse

Koettnitz

Merten

Kronenberg³

et al. 2021

Materials

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Bone substitute materials are becoming increasingly important in oral and maxillofacial surgery. Reconstruction of critical size bone defects is still challenging for surgeons. Here, we compared the clinically applied organic bone substitute materials NanoBone® (nanocrystalline hydroxyapatite and nanostructured silica gel; n = 5) and Actifuse (calcium phosphate with silicate substitution; n = 5) with natural collagen-based Spongostan™ (hardened pork gelatin containing formalin and lauryl alcohol; n = 5) in bilateral rat critical-size defects (5 mm diameter). On topological level, NanoBone is known to harbour nanopores of about 20 nm diameter, while Actifuse comprises micropores of 200–500 µm. Spongostan™, which is clinically applied as a haemostatic agent, combines in its wet form both nano- and microporous topological features by comprising 60.66 ± 24.48 μm micropores accompanied by nanopores of 32.97 ± 1.41 nm diameter. Micro-computed tomography (µCT) used for evaluation 30 days after surgery revealed a significant increase in bone volume by all three bone substitute materials in comparison to the untreated controls. Clearly visual was the closure of trepanation in all treated groups, but granular appearance of NanoBone® and Actifuse with less closure at the margins of the burr holes. In contrast, transplantion of Spongostan™ lead to complete filling of the burr hole with the highest bone volume of 7.98 ccm and the highest bone mineral density compared to all other groups. In summary, transplantation of Spongostan™ resulted in increased regeneration of a rat calvarial critical size defect compared to NanoBone and Actifuse, suggesting the distinct nano- and microtopography of wet Spongostan™ to account for this superior regenerative capacity. Since Spongostan™ is a clinically approved product used primarily for haemostasis, it may represent an interesting alternative in the reconstruction of defects in the maxillary region.

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Selected Nanomaterials’ Application Enhanced with the Use of Stem Cells in Acceleration of Alveolar Bone Regeneration during Augmentation Process

Cited by 38 publications

References 205 publications

Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment

Ipriflavone-Loaded Mesoporous Nanospheres with Potential Applications for Periodontal Treatment

A Modified Ridge Splitting Technique Using Autogenous Bone Blocks—A Case Series

Spongostan™ Leads to Increased Regeneration of a Rat Calvarial Critical Size Defect Compared to NanoBone® and Actifuse

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