Selected qualitative and biochemical parameters of cryopreserved semen of Holstein-Friesian (HF) AI bulls

Lecewicz, Marek; Hering, Dorota; Kamiński, Stanisław; Majewska, Anna; Kordan, Władysław

doi:10.1515/pjvs-2015-0030

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…The progressive motility of frozen Sahiwal bull semen reported in this study agrees with the findings of Lecewicz et al (2015); Dolezalova (2016); Baloch et al (2019) and Mostari et al (2004). However, the findings of the present study are lower than the findings of some previous research works (Sugulle et al, 2006;Ansari et al, 2010;Gopinthan et al, 2018) and higher than the findings of Ulfina et al (2002).…”

Section: Evaluation Of Frozen Semensupporting

confidence: 85%

Frozen semen quality and fertility of imported pure Holstein Friesian and Sahiwal breeding bulls in Bangladesh

Hasan

Islam

Husain

et al. 2020

Asian Australas. J. Biosci. Biotechnol.

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The present study was conducted to evaluate the frozen semen quality in terms of different motility patterns and fertility of imported pure Holstein Friesian and Sahiwal breeding bulls maintained under farming condition of Bangladesh. 100% pure Holstein Friesian and Sahiwal breeding bulls were imported and thereafter reared in the American dairy limited (ADL). Semen samples were collected once a week, processed and frozen in the laboratory of ADL. On the day of collection, semen volume and sperm concentration measured immediately after collection. Different motility patterns of spermatozoa (progressive, fast, slow, local, immotile) were measured by Computer Assisted Semen Analyzer (CASA). Effect of breed was found significant (p<0.05) on semen volume and sperm concentration. The semen volume (ml/ejaculate) and sperm concentration (×106) were found to be 6.13±0.28 and 1664.28±62.41 in Holstein Friesian breeding bulls whereas 5.26±0.17 and 2036.00±43.99 in Sahiwal bulls, respectively. The progressive motility, fast motility, slow motility and local motility were significantly (p<0.05) higher in Holstein Friesian bulls than that of Sahiwal bulls both before and after freezing. Different motility patterns such as progressive, fast, slow, local and immotile motility were found to be 89.22±0.45%, 75.97±0.67%, 9.10±0.36%, 4.25±0.15% and 10.05±0.41% in Holstein Friesian bulls whereas 84.98±0.89%, 65.99±0.92%, 11.88±0.37%, 5.65±0.19% and 15.69±0.54% in Sahiwal breeding bulls, respectively before freezing. On the other hand, after freezing, the progressive, fast, slow, local and immotile spermatozoa were found to be 68.19±0.46%, 53.39±2.01%, 9.21±0.46%, 8.47±0.72% and 26.76±2.01% in Holstein Friesian bulls whereas 56.54±0.25%, 42.52±0.67%, 11.11±0.55%, 13.04±1.24% and 30.45±1.58% in Sahiwal bulls, respectively. Non-return rate was found insignificant between the breeds and it was 64.19% in Holstein Friesian and 64.71% in Sahiwal bulls. In conclusion, frozen semen quality of Holstein Friesian pure breed is better than that of Sahiwal breeding bulls but fertility of both breeds are similar. Therefore, frozen semen of both breeds can be used in the field level for the genetic improvement of cattle. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (1), 33-41

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Section: Evaluation Of Frozen Semensupporting

confidence: 85%

Frozen semen quality and fertility of imported pure Holstein Friesian and Sahiwal breeding bulls in Bangladesh

Hasan

Islam

Husain

et al. 2020

Asian Australas. J. Biosci. Biotechnol.

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“…Los valores encontrados para la actividad enzimática antioxidante del semen criopreservado (Cuadro 1) fueron superiores a los reportados por Lecewicz et al (2015). Sin embargo, se conoce que la actividad enzimática antioxidante podría incrementarse por el aporte de los diferentes componentes de los diluyentes, como la YH (Restrepo et al, 2016); un medio rico en antioxidantes no enzimáticos como la vitamina E, la vitamina B12, la biotina y la fosvitina (Seuss-Baum, 2007).…”

Section: Resultados Y Discusiónunclassified

Efecto de las lipoproteínas de baja densidad y la trehalosa sobre la actividad enzimática antioxidante del semen bovino criopreservado

Rojano

Betancur

2019

Rev. investig. vet. Perú

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El objetivo de este estudio fue evaluar la capacidad antioxidante enzimática del semen bovino criopreservado con diferentes fuentes de lipoproteínas de baja densidad (LDL) y trehalosa (T). Diez eyaculados seleccionados a partir de cinco toros fueron criopreservados bajo los tratamientos: LDL (8% m/v), T (100 mM), TLDL (T 100 mM y LDL 8% m/v), yema de huevo centrifugada (YHC, 20% v/v) y yema de huevo (YH, 20% v/v) como tratamiento control. Se evaluó la capacidad enzimática antioxidante de la catalasa (CAT), la superóxido dismutasa (SOD) y la glutatión peroxidasa (GPx) del semen bovino luego de la descongelación por espectrofotometría y su relación con los parámetros cinéticos espermáticos. Se utilizó un modelo completamente al azar. La normalidad de las variables se validó mediante la prueba de Kolmogorov-Smirnov. Las fuentes de variación se evaluaron a través de un modelo mixto. La comparación de las medias entre los tratamientos se llevó a cabo utilizando la prueba de Tukey. Los tratamientos YH, YHC y LDL aumentaron la actividad antioxidante de CAT y GPx y se correlacionaron positivamente con los parámetros cinéticos y la integridad de la membrana espermática (p<0.05). No se encontraron diferencias entre LDL y TLDL con respecto a la actividad de CAT y GPx. La actividad enzimática de SOD fue similar en todos los tratamientos en comparación con el control (YH). T no mejoró la actividad antioxidante de las enzimas utilizadas. Se concluye que los diluyentes con YH, YHC y LDL mejoran la actividad antioxidante de CAT y GPx, generando una correlación positiva con la calidad seminal.

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“…SOD and GPX activity were previously measured in ram seminal plasma: SOD and GPX levels were higher in rams supplemented with vitamin C and E by oral administration, in comparison to the control group (rams without oral supplementation) [ 23 ]. Different authors described low levels of these enzymatic antioxidants were related with high DNA damage in bulls [ 24 ] and low motility in stallions [ 25 ]. In recent years, different analyses related to oxidative stress have been described considering the beneficial and detrimental role of ROS and considering the redox balance as a more accurate parameter in oxidative stress studies [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…The MiOXSYS ® system is a new technology to conjointly evaluate redox balance by a galvanostatic-method [ 27 ]. The MiOXSYS ® system allows detection of variations in oxidant capacity in semen samples [ 24 ]. To summarize, the analysis of oxidative stress should be addressed with different oxidative stress markers, such as enzymatic activity of antioxidants located in seminal plasma (SOD, GPX, CAT), and through considering redox balance in a global way.…”

Section: Introductionmentioning

confidence: 99%

Frequency of Semen Collection Affects Ram Sperm Cryoresistance

Palacin-Martinez

Álvarez

Montes-Garrido

et al. 2022

Animals

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The improvement of frozen-thawed sperm quality has been mostly approached from the view of cryopreservation protocol optimization in terms of cryoprotectant solutions, freezing-thawing rates and antioxidant supplementation, while the impact of sperm collection frequency remains unknown in rams. In this work, a multiparametric study was carried out in cooled and frozen-thawed semen to evaluate sperm quality after different semen collection frequencies during a month: zero sperm collection (0 CW), four sperm collections per week (4 CW), and ten sperm collections per week (10 CW). Traditional analyses have been applied, in combination with novel technologies related to redox balance. Frozen-thawed semen quality showed a significant decrease (p < 0.05) in 0 CW and 10 CW in comparison to 4 CW, concerning motility and kinetics parameters. However, apoptosis showed a significant increase (p < 0.05) in 10 CW in comparison to 0 CW and 4 CW. The employment methods related to redox balance provided us with the definitive probe to ensure the influence of collection frequency on balance redox after thawing. Specifically, glutathione peroxidase (GPX) and superoxide dismutase (SOD) activity showed a significant decrease (p < 0.05) in 10 CW compared to 0 CW and 4 CW. The characterization of alternative strategies to sperm cryopreservation based on consideration of male sexual regimes, could improve the quality of frozen-thawed sperm.

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Selected qualitative and biochemical parameters of cryopreserved semen of Holstein-Friesian (HF) AI bulls

Cited by 4 publications

References 6 publications

Frozen semen quality and fertility of imported pure Holstein Friesian and Sahiwal breeding bulls in Bangladesh

Frozen semen quality and fertility of imported pure Holstein Friesian and Sahiwal breeding bulls in Bangladesh

Efecto de las lipoproteínas de baja densidad y la trehalosa sobre la actividad enzimática antioxidante del semen bovino criopreservado

Frequency of Semen Collection Affects Ram Sperm Cryoresistance

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