Selected Wild Plants Ethanol Extracts Bioactivity on the Coagulation Cascade

Omar, Ghadeer; Abdallah, Lubna; Rahim, Ahmad Abdal; Othman, R.; Barakat, Ali

doi:10.9734/jsrr/2017/32989

Cited by 13 publications

(14 citation statements)

References 30 publications

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“…For example, lack of proper clot formation may evoke excessive bleeding. To our current knowledge, there is no data regarding plantderived EVs effect on hemostasis; however, from pharmacognosy and phytochemistry sciences, it is known that some plants have anti-bleeding properties [167]. Also, many examples of hemostatic plants, such as Rubia cordifolia (Manjistha) [168], Ageratum conyzoides, Alchornea cordifelia, Aspilia africana, Baphia nitida, Chromolaena odorata, Jathropha curcas, Landolphia owariensis are identified in the literature.…”

Section: Plant-derived Extracellular Vesicles In Hemostasismentioning

confidence: 99%

Extracellular Vesicles in Skin Wound Healing

Narauskaitė

Vydmantaitė

Rusteikaitė

et al. 2021

Preprint

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Each year, millions of individuals suffer from a non-healing wound, abnormal scarring, or injuries accompanied by an infection. For these cases, scientists are searching for new therapeutic interventions, from which one of the most promising is the use of extracellular vesicles (EVs). Naturally, EV-based signalling takes part in all four wound healing phases: hemostasis, inflammation, proliferation and remodelling. Such an extensive involvement of EVs suggests exploiting their action to modulate the impaired healing phase. Furthermore, next to their natural wound healing capacity, EVs can be engineered for better defined pharmaceutical purposes, such as carrying specific cargo or targeting specific destinations by labelling them with certain surface proteins. This review aims to promote scientific awareness in basic and translational research of EVs by summarizing the current knowledge about their natural role in each stage of skin repair and the most recent findings in application areas, such as wound healing, skin regeneration and treatment of dermal diseases, including the stem cell-derived, plant-derived and engineered EVs.

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Section: Plant-derived Extracellular Vesicles In Hemostasismentioning

confidence: 99%

Extracellular Vesicles in Skin Wound Healing

Narauskaitė

Vydmantaitė

Rusteikaitė

et al. 2021

Preprint

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“…All results were obtained by a digital coagulation analyzer (coagulation analyzer Coa-DATA 4004, Germany). All measurements were conducted in duplicate and 1% DMSO was used as a negative control (15).…”

Section: Microbial Isolatesmentioning

confidence: 99%

Antimicrobial, Anticoagulant and Anticancer Effects of Arum Palaestinum Flowers Extracts

Dwikat

Jaradat

Amer

et al. 2021

Preprint

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Background: Wild plants are amply utilized in traditional medicine and folkloric food worldwide. Arum palaestinum Boiss. (AP) is one of the wild Palestinian plants which leaves have a long history in the Middle Eastern countries as food and medicine. Herby, the current study aimed to evaluate the antimicrobial, coagulation cascade activities, and anticancer effects of (AP) flowers extract Methods: The aqueous extract of (AP) flowers was screened on its antimicrobial activity using microdilution assay against eight pathogens. While, prothrombin time, activated partial thromboplastin time, and thrombin time tests were measured utilizing standard hematological methods. And Anti cancer effect was assessed by using Parameters of cell cycles and alph feta protein level that were investigated for (AP) flowers fractionated with aqueous, DMSO, and methanol Results: The antimicrobial screening results revealed that the aqueous extract of (AP) has strong antibacterial effects against P. vulgaris and E. faecium compared with Ampicillin with MIC values of 6.25, 6.25 and 18 mg/ml, respectively. The aqueous extract of (AP) showed anticoagulant activity with significant prolonged results in aPTT and TT tests at high concentrations (50 mg/ml and 25 mg/ml) and slightly prolonged results in the PT test at a high concentration (50 mg/ml). The anticancer results indicate a delay in cell cycle through decreased the cell proliferation rate following effects of the AP fractions. The delay in the S phase was in favor of the water fraction. Water and DMSO fractions while maintained the cells in the G2-M phase similar to the DOX, the flower extract in methanol accelerated the cells in the G2-M phase suggesting that (AF) flower extracts have anti-cancer properties. At the same time Aqueous extract decreased HCC aFP to 1.55-fold (P=0.0008). While DMSO and methanolic extract had no significant effects on HCC aFP levels, compared to control untreated cells of 2519.16 ± 198.1 ng/ml. This data show that (AF) aqueous solution is potent inhibitor of alpha-fetoprotein secretion (P-value <0.05), which indicates its anti-carcinogenic effects Conclusion: These results showed that the aqueous extract of (AP) plant possesses bioactive components with antibacterial and anticoagulant properties, which may be exploited in the treatment of infectious diseases and blood coagulation disorders.

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“…However, for alcoholic extraction, ten grams of each plant powder were soaked in 100 mL of (70%) ethanol or methanol for one week with interval shaking. Then the same steps of the aqueous extraction procedure were repeated, except that (1%) dimethyl sulfoxide (DMSO) was used as a solvent and evaporation was performed by a rotary evaporator (Omar et al, 2017).…”

Section: Plant Extraction Proceduresmentioning

confidence: 99%

“…In 2014, Viscum album extracts from olive and almond host plants were proved to have significant prolongation effect on PT and aPTT (Abualhasan et al, 2014). Later on, another in vitro study showed that the ethanol extracts of Urtica urens, Parietaria judica, Satureja thymbra, Thymbra spicata, Teucrium creticum, Verbascum fruticulosum, Lupinus pilosus, Paronychia argentea, and Ruta chalepensis had either anticoagulation or coagulation effect on the examined blood samples (Omar et al, 2017). A wide spectrum of different phytochemicals could be extracted using various solvents due to variations in their solubility and polarity (Liu, 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro haemostatic efficacy of aqueous, methanol and ethanol plant extracts of three medicinal plant species in Palestine

et al. 2020

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The haemostatic efficacy of different extract types of Satureja thymbra L., Thymbra spicata L. (Lamiaceae) and Verbascum fruticulosum Post. (Scrophulariaceae) was evaluated in this study via the Prothrombin time (PT) and Activated partial thromboplastin time (aPTT) analysis. Aqueous, methanol and ethanol extracts of the examined plant species leaves were prepared to a final concentration 50 mg/mL. In vitro PT and aPTT assays were conducted on normal platelet poor plasma blood samples by a digital coagulation analyzer. The obtained results revealed anticoagulation activity of all investigated plant species with observed variations among them. The aqueous and ethanol extracts of T. spicata as well as the aqueous extract of S. thymbra prolonged PT values significantly (p < 0.05). While, all V. fruticulosum extract types have had no significant effect on the PT values. The recorded aPTT data showed that all aqueous extracts have had a significant effect on the blood haemostasis as they increased aPTT values in all plant species under study. Out of which, both the ethanol and methanol extracts of T. spicata and methanol extract of S. thymbra showed similar effect. Of great concern, it was clearly noticed that the aqueous and ethanol extract of T. spicata and the aqueous extract of S. thymbra possess the strongest anticoagulation effect as they increased both PT and aPTT values significantly relative to the control (p < 0.05). The variable anticoagulation bioactivity among the studied plant species could be referred to the various solvents degrees of solubility of different phyto-constituents. Thus, the efficacy of the plant species extracts evaluation as anticoagulants or coagulants were related to the plant species and to the solvent of extraction.

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Selected Wild Plants Ethanol Extracts Bioactivity on the Coagulation Cascade

Abstract: Aim: As there is little data available on the validity of wild plants use in Palestine for blood disorders, the aim of this study was to determine the anticoagulant properties of Urtica urens,

Cited by 13 publications

References 30 publications

Extracellular Vesicles in Skin Wound Healing

Extracellular Vesicles in Skin Wound Healing

Antimicrobial, Anticoagulant and Anticancer Effects of Arum Palaestinum Flowers Extracts

In vitro haemostatic efficacy of aqueous, methanol and ethanol plant extracts of three medicinal plant species in Palestine

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