Selectin Blockade Reduces Neutrophil Interaction With Platelets at the Site of Deep Arterial Injury by Angioplasty in Pigs

Merhi, Yahye; Provost, Patrick; Chauvet, Patrick; Théorêt, Jean-François; Phillips, M. L.; Latour, Jean-Gilles

doi:10.1161/01.atv.19.2.372

Cited by 33 publications

(37 citation statements)

References 59 publications

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“…31 The balloon was positioned under fluoroscopic control into the left and right common carotid arteries. Five 30-second inflations were performed at a pressure of 6 atm, with resting intervals of 60 seconds between each inflation.…”

Section: Double Arterial Injury By Angioplasty and Experimental Groupsmentioning

confidence: 99%

“…Before and within 10 minutes of treatment administration, heart rate, blood pressure, hematological parameters, activated clotting time (ACT), and platelet aggregation were determined in each animal, as described previously. 31 …”

Section: Double Arterial Injury By Angioplasty and Experimental Groupsmentioning

confidence: 99%

“…8,9,31 Briefly, a low-speed centrifuge yielded platelet-rich plasma, from which platelets were isolated and incubated with 300 Ci of 51 Cr for 30 minutes. Next, the suspension was centrifuged to remove unbound 51 Cr, resuspended in platelet-poor plasma, and reinjected into the animal.…”

Section: Isolation and Labeling Of Neutrophils And Plateletsmentioning

confidence: 99%

“…The amount of neutrophils and platelets per square centimeter adhering to arterial segments was calculated from the radioactivity of the segment and from the cellular count and radioactivity of a reference blood sample, corrected for the luminal surface of each arterial segment, as described previously. 31,32 The results are presented as platelet and neutrophil adhesion values from injured segments minus the adhesion on uninjured distal reference segments.…”

Section: Quantification Of Neutrophil and Platelet Adhesionmentioning

confidence: 99%

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Recombinant Soluble P-Selectin Glycoprotein Ligand-1-Ig Reduces Restenosis Through Inhibition of Platelet-Neutrophil Adhesion After Double Angioplasty in Swine

Bienvenu

Tanguay²,

Théorêt³

et al. 2001

Circulation

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Background-P-selectin mediates leukocyte recruitment to activated platelets and endothelium through its high-affinity receptor P-selectin glycoprotein ligand-1 (PSGL-1). Platelet and leukocyte activation and binding have been reported after coronary angioplasty and were correlated with restenosis. We investigated the effect of a recombinant soluble PSGL-1 (rPSGL-Ig) on the adhesion of platelets and neutrophils and the development of restenosis after double arterial injury. Methods and Results-Four weeks after angioplasty of both carotid arteries in pigs, a second angioplasty was performed at the same sites, 15 minutes after a single administration of vehicle or rPSGL-1 (1 mg/kg IV). Animals were euthanized 1 hour, 4 hours, 1 week, or 4 weeks later. Adhesion of autologous 51 Cr-platelets and 111 In-neutrophils was quantified and histological/morphometric analyses were performed. Although rPSGL-Ig did not affect adherence of these cells 1 hour after injury, it significantly reduced the adhesion of platelets (50% at 4 hours and 85% at 1 week) and neutrophils (50% at 4 hours and 78% at 1 week) to deeply injured arteries. At 4 weeks, the residual lumen was 63% larger in rPSGL-Ig-treated arteries as compared with control arteries (6.1Ϯ0.6 versus 3.8Ϯ0.1 mm 2 ; PϽ0.002). The neointimal area was slightly reduced (0.5 in rPSGL-Ig versus 0.7 mm 2 in control). The ratio of the external elastic lamina of injured to uninjured reference segments was Ͼ1 in treated arteries and Ͻ1 in control arteries. Conclusions-P-selectin antagonism with rPSGL-Ig inhibits early platelet/leukocyte adhesion on injured arteries and reduces restenosis through a positive impact on vascular remodeling. Hence, rPSGL-Ig may have potential in the prevention of restenosis.

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Section: Double Arterial Injury By Angioplasty and Experimental Groupsmentioning

confidence: 99%

Section: Double Arterial Injury By Angioplasty and Experimental Groupsmentioning

confidence: 99%

Section: Isolation and Labeling Of Neutrophils And Plateletsmentioning

confidence: 99%

Section: Quantification Of Neutrophil and Platelet Adhesionmentioning

confidence: 99%

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Recombinant Soluble P-Selectin Glycoprotein Ligand-1-Ig Reduces Restenosis Through Inhibition of Platelet-Neutrophil Adhesion After Double Angioplasty in Swine

Bienvenu

Tanguay²,

Théorêt³

et al. 2001

Circulation

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“…In human studies, P-selectin levels were shown to be significantly increased in plasma in patients with acute myocardial infarct (34). The use of a selectin blocker (CY-1503), an analogue of sialyl Lewis X selectin ligand, inhibited leukocyte and platelet interaction after arterial injury produced by angioplasty in pigs (35). Other studies have shown that the selectin blocker fucoidin provides cardioprotection in rats and dogs with coronary artery occlusion (36).…”

Section: Heartmentioning

confidence: 99%

Pathophysiologic role of selectins and their ligands in ischemia reperfusion injury

Chamoun¹

2000

Front Biosci

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Polymorphonuclear neutrophils and instability of the atherosclerotic plaque: a causative role?

et al. 2013

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Activated PMNs were shown to produce and release reactive oxygen species, inflammatory leukotrienes and proteolytic lysosomal enzymes, directly inducing vascular damage. Activated PMNs also secrete myeloperoxidase, involved in lipoprotein oxidation. PMNs have a finite lifespan and typically die through apoptosis, which thus represents a counter-regulatory mechanism limiting the toxic potential of these short-lived, terminally differentiated cells. Dysregulation of this process probably contributes to the pathogenesis and progression of several inflammatory diseases. Moreover, high circulating levels of PMN-platelet aggregates have been reported in patients with clinical atherosclerosis, and recent studies suggest that these aggregates may play a role in vascular response to injury. It has been suggested that this heterotypic interaction between platelets and leukocytes might represent a link between hemostasis/thrombosis and the inflammatory response.

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Selectin Blockade Reduces Neutrophil Interaction With Platelets at the Site of Deep Arterial Injury by Angioplasty in Pigs

Cited by 33 publications

References 59 publications

Recombinant Soluble P-Selectin Glycoprotein Ligand-1-Ig Reduces Restenosis Through Inhibition of Platelet-Neutrophil Adhesion After Double Angioplasty in Swine

Recombinant Soluble P-Selectin Glycoprotein Ligand-1-Ig Reduces Restenosis Through Inhibition of Platelet-Neutrophil Adhesion After Double Angioplasty in Swine

Pathophysiologic role of selectins and their ligands in ischemia reperfusion injury

Polymorphonuclear neutrophils and instability of the atherosclerotic plaque: a causative role?

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