Selection and characterisation of an <scp>HD1</scp>‐like <i>Bacillus thuringiensis</i> isolate with a high insecticidal activity against <i>Spodoptera littoralis</i> (Lepidoptera: Noctuidae)

Azzouz, Hichem; Kebaili-Ghribi, J.; Farhat-Touzri, Dalel Ben; Daoud, F.; Fakhfakh, Ines; Tounsi, Slim; Jaoua, Samir

doi:10.1002/ps.3661

Cited by 12 publications

(7 citation statements)

References 77 publications

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“…The insecticidal activity of spore/crystal mixtures is an effective way to search for native Bt isolates having new entomocidal properties [ 7 , 32 , 34 , 35 , 36 ]. In our study, preliminary assays indicated that some of the 18 selected isolates in the collection had high toxicity against Lepidoptera.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Bacillus thuringiensis isolates by their insecticidal activity and their production of Cry and Vip3 proteins

et al. 2018

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Bacillus thuringiensis (Bt) constitutes the active ingredient of many successful bioinsecticides used in agriculture. In the present study, the genetic diversity and toxicity of Bt isolates was investigated by characterization of native isolates originating from soil, fig leaves and fruits from a Turkish collection. Among a total of 80 Bt isolates, 18 of them were found carrying a vip3 gene (in 23% of total), which were further selected. Insecticidal activity of spore/crystal mixtures and their supernatants showed that some of the Bt isolates had significantly more toxicity against some lepidopteran species than the HD1 reference strain. Five isolates were analyzed by LC-MS/MS to determine the Cry protein composition of their crystals. The results identified the Cry1Ac protein and a Cry2A-type protein in all isolates, Cry1Ea in 3 of them and Cry1Aa in one. The sequence analysis of the new vip3 genes showed that they had a high similarity to either vip3Aa, vip3Af or vip3Ag (94–100%). The vip3Aa gene of the 6A Bt isolate was cloned and sequenced. The protein was named Vip3Aa65 by the Bacillus thuringiensis Nomenclature Committee. The expressed and purified Vip3Aa65 protein was tested against five lepidopteran species and its toxicity compared to that of a reference protein (Vip3Aa16). Both proteins had similar toxicity against Grapholita molesta and Helicoverpa armigera, whereas Vip3Aa65 was less active than Vip3Aa16 against three species from the Spodoptera genus. A tetrameric structure of the Vip3Aa65 protein was detected by gel filtration chromatography. The study revealed some isolates with high insecticidal activity which can be considered promising candidates to be used in pest control.

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Section: Discussionmentioning

confidence: 99%

Characterization of Bacillus thuringiensis isolates by their insecticidal activity and their production of Cry and Vip3 proteins

et al. 2018

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“…The Egyptian cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is a native pest to Africa (Shonouda and Osman, 2000;El-Khawas and Abd El-Gawad, 2002), and distributed in many European countries (Pineda et al, 2007;EPPO, 2019), Asia Minor and the Middle East countries (El-Aswad, 2007;El-Sabrout, 2013;Azzouz et al, 2014). Economically, it is a dangerous pest of many field crops and vegetables in North Africa, Middle East countries including Egypt (Kandil et al, 2003) and glasshouses plants and flowers production in Southern Europe (Roques et al, 2008;Abdel-Mageed et al, 2018), as well as various cash and traditional food crops in Africa (Capinera, 2008;Khedr et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Threshold Temperatures and Thermal Requirements of Cotton Leafworm, Spodoptera littoralis Hb.

Allam

Manaa

Dahi

et al. 2021

Egyptian Academic Journal of Biological Sciences. A, Entomology

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“…Although Egyptian cotton leafworm Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is a native pest to Africa (Shonouda and Osman, 2000;El-Khawas and Abd El-Gawad, 2002), it is distributed in many European countries (Pineda et al, 2007;Lanzoni et al, 2012;EPPO, 2019), Asia Minor and the Middle East countries (El-Aswad, 2007;El-Sabrout, 2013;Azzouz et al, 2014). S. littoralis caterpillars feed on more than 90 plant species of economic importance belonging to 44 plant families (Kandil et al, 2003;, such as vegetables, horticultural crops and ornamental plants (Dahi, 2005;Amin, 2007;Ismail, 2014;Sut et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Impairing Effectiveness of Nerolidol, a Sesquiterpene Compound, on Adult Performance and Reproductive Potential of Egyptian Cotton Leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae).

Hamadah

Ghoneim

Waheeb

2020

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Although Egyptian cotton leafworm Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) is native in Africa, it is distributed throughout the world. It is a serious polyphagous insect damaging more than 90 host plants of economic importance. The objective of the current study was to evaluate the disruptive impact of Nerolidol, a Sesquiterpene compound, on the most important parameters of adult performance and reproductive potential of this insect. The newly moulted larvae of 5 th (penultimate) or 6 th (last) instar larvae fed castor bean leaves previously treated with 7 concentrations of Nerolidol (400, 200, 100, 50, 25, 12.5 & 6.25 ppm) for 24 hr. The most important results could be summarized as follows. Nerolidol exhibited an adulticidal activity only at the higher concentrations. Nerolidol exerted an anti-morphogenic activity against adult moths since some malformed adults were produced at the higher concentrations. Regardless the treated larval instar, Nerolidol induced the successfully emerged adults to live remarkably shortened total longevity and oviposition period, but the pre-oviposition period was generally prolonged. Nerolidol exhibited an inhibitory effect on the oviposition efficiency since the oviposition rate was deleteriously regressed, in a dose-dependent course. Nerolidol caused a disturbance of the reproductive capacity since fecundity and fertility were dramatically prohibited. After larval treatment with Nerolidol, the successfully mated adult females laid eggs with a significantly prolonged incubation period, especially at the higher concentration levels.

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Selection and characterisation of an HD1‐like Bacillus thuringiensis isolate with a high insecticidal activity against Spodoptera littoralis (Lepidoptera: Noctuidae)

Abstract: On the basis of its potent insecticidal activity and its high level of δ-endotoxin production, the BUPM28 isolate can be considered to be an effective alternative for the control of S. littoralis.

Cited by 12 publications

References 77 publications

Characterization of Bacillus thuringiensis isolates by their insecticidal activity and their production of Cry and Vip3 proteins

Characterization of Bacillus thuringiensis isolates by their insecticidal activity and their production of Cry and Vip3 proteins

Threshold Temperatures and Thermal Requirements of Cotton Leafworm, Spodoptera littoralis Hb.

Impairing Effectiveness of Nerolidol, a Sesquiterpene Compound, on Adult Performance and Reproductive Potential of Egyptian Cotton Leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae).

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