1984
DOI: 10.1104/pp.76.3.571
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Selection and Characterization of a Carrot Cell Line Tolerant to Glyphosate

Abstract: Cultured carrot (Daucs carota L.) cells were adapted to growing in 25 millimolar glyphosate by transfer into progressively higher concentrations ofthe herbicide. Tolerance was increased 52-fold, and the adaptation was stable in the absence of glyphosate. The uptake of glyphosate was similar for adapted and nonadapted cells. Activity of the enzyme 5-enolpyruvylshikimic acid-3-phosphate synthase was 12-fold higher in the adapted line compared to nonadapted cells, while activities of shikimate dehydrogenase and a… Show more

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Cited by 120 publications
(55 citation statements)
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“…An alfalfa (Medicago sativa L.) cell line that had a 60-to 100-fold increase in tolerance to PPT, an inhibitor of GS, only exhibited a 3-to 7-fold increase in herbicide-sensitive GS activity (8). A glyphosate-tolerant carrot (Daucus carota) cell line exhibited a 50-fold increase in tolerance and a 12-fold increase in EPSP synthase activity compared with the control (21). Alternatively, closer correspondence of EPSP synthase overproduction and glyphosate tolerance level were reported for a glyphosate-tolerant Petunia hybrida cell line (28,30).…”
Section: Acetyl-coenzyme a Carboxylase Activitymentioning
confidence: 98%
“…An alfalfa (Medicago sativa L.) cell line that had a 60-to 100-fold increase in tolerance to PPT, an inhibitor of GS, only exhibited a 3-to 7-fold increase in herbicide-sensitive GS activity (8). A glyphosate-tolerant carrot (Daucus carota) cell line exhibited a 50-fold increase in tolerance and a 12-fold increase in EPSP synthase activity compared with the control (21). Alternatively, closer correspondence of EPSP synthase overproduction and glyphosate tolerance level were reported for a glyphosate-tolerant Petunia hybrida cell line (28,30).…”
Section: Acetyl-coenzyme a Carboxylase Activitymentioning
confidence: 98%
“…Glyphosate has been shown to inhibit methionine synthesis in plants, in addition to the aromatic amino acids [99]. We have already discussed glyphosate's inhibition of assimilatory sulfite reductase in the common gut microbe E. coli, and this is an essential enzyme in the methionine synthesis pathway.…”
Section: Homocysteine Thiolactonementioning
confidence: 99%
“…Hence, suppression of this enzyme leads to methionine deficiency in addition to the accumulation of toxic amounts of sulfite in the gut. Glyphosate's known inhibition of methionine synthesis in plants [99] may plausibly be due at least in part to suppression of assimilatory sulfite reductase.…”
Section: Molybdenum Deficiency and Sulfitementioning
confidence: 99%
“…The mutagenicity of methylglyoxal is suppressed by sulfur-containing molecules, such as sulfite, cysteine and glutathione [96]. Glyphosate has been shown to deplete methionine levels by 50% to 65% in a glyphosate-sensitive carrot plant line [97]. Methionine is an essential sulfur-containing amino acid crucial for maintaining levels of cysteine, glutathione and sulfate.…”
Section: Glyoxal Methylglyoxal and Glyoxylatementioning
confidence: 99%