Selection and Characterization of Endophytic Bacteria as Biocontrol Agents of Tomato Bacterial Wilt Disease

Nawangsih, Abdjad Asih; Damayanti, Ika; Wiyono, Suryo; Kartika, Juang Gema

doi:10.4308/hjb.18.2.66

Cited by 42 publications

(26 citation statements)

References 12 publications

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“…Isolate AB131-1 and LBR02 produced siderophore. The ability to produce siderophores is one of the characters that make microorganisms successful competitors in several environments because, in sufficient quantities, it may limit Fe 3+ availability to the pathogen and influence the induction of host resistance against the pathogen (Meziane et al 2005). Streptomyces spp.…”

Section: Discussionmentioning

confidence: 99%

Endophytic Streptomyces spp. as Biocontrol Agents of Rice Bacterial Leaf Blight Pathogen (Xanthomonas oryzae pv. oryzae)

Hastuti

Lestari

Suwanto

et al. 2012

HAYATI Journal of Biosciences

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Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants inoculated with only Streptomyces spp. Plants inoculated with Xoo and sprayed with a chemical bactericide, and plants inoculated with only Xoo served as positive controls, whereas plants not inoculated with either Streptomyces spp. or Xoo were used as negative controls. The results showed that the effect of endophytic Streptomyces spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P > 0.05). However, plants inoculated with endophytic Streptomyces spp. were significantly taller and produced higher tiller number than control plants (P < 0.05). Streptomyces spp. isolate AB131-1 gave the highest plant height. In vitro studies on biocontrol mechanisms of selected Streptomyces spp. isolates showed that isolate LBR02 gave the highest inhibition activity on Xoo growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic Streptomyces spp. from different species. These studies found that the paddy plant which was inoculated with endophytic Streptomyces spp. AB131-1 and infected by Xoo could increase the height of plant and number of tillers.

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Section: Discussionmentioning

confidence: 99%

Endophytic Streptomyces spp. as Biocontrol Agents of Rice Bacterial Leaf Blight Pathogen (Xanthomonas oryzae pv. oryzae)

Hastuti

Lestari

Suwanto

et al. 2012

HAYATI Journal of Biosciences

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“…Uninoculated control leaves were injected with SDW only (negative control). Tobacco plants (inoculated and uninoculated) were incubated at room temperature for 24 h. Isolates leading to the formation of chlorotic and/or necrotic zones on the inoculated leaf areas were considered as pathogenic and they were excluded from further screening bioassays (Nawangsih et al 2011).…”

Section: Hypersensitivity Test Of Endophytic Bacterial Isolatesmentioning

confidence: 99%

Biocontrol of Fusarium Wilt and Growth Promotion of Tomato Plants Using Endophytic Bacteria Isolated from Solanum elaeagnifolium Stems

Abdallah

Jabnoun‐Khiareddine

Nefzi

et al. 2016

Journal of Phytopathology

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Seven culturable bacterial isolates, obtained from the internal stem tissues of Solanum elaeagnifolium and successfully colonizing the internal stem tissues of tomato cv. Rio Grande, were screened for their in vivo antifungal activity against Fusarium oxysporum f.sp. lycopersici (FOL) and their growth‐promoting potential on tomato plants. SV101 and SV104 isolates, assessed on pathogen‐challenged tomato plants led to a significant decrease (77–83%) in Fusarium wilt severity and vascular browning extent (76%), as compared to the inoculated and untreated control. Isolates enhanced growth parameters on pathogen‐challenged and unchallenged tomato plants. SV104 and SV101 isolates were most effective in suppressing disease and enhancing plant growth. These two isolates were identified as Bacillus sp. str. SV101 () and B. tequilensis str. SV104 (). They displayed antifungal activity against FOL; pathogen growth was inhibited by 64% and an inhibition zone (11.50 and 19.75 mm) against FOL could be formed using whole cell suspensions. SV101 and SV104 extracellular metabolites also inhibited FOL growth by 20 and 55%, respectively, as compared to control. B. tequilensis str. SV104 was shown to produce protease, chitinase, pectinase, IAA and siderophores. Bacillus sp. str. SV101 displayed pectinase activity and was found to be an IAA‐producing and phosphate‐solubilizing agent. To our knowledge, this is the first study reporting on S. elaeagnifolium use as a potential source of potent biocontrol and plant growth‐promoting agents.

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“…Agens biokontrol yang dapat digunakan sebagai alternatif pengendalian dalam rangka mewujudkan sistem pertanian yang berkelanjutan adalah bakteri endofit dan rizobakteria pemacu pertumbuhan tanaman (RPPT) (Sutariati et al 2006;Nawangsih et al 2011). Bakteri endofit adalah bakteri yang mengolonisasi jaringan tanaman sehat tanpa menyebabkan penyakit pada inangnya, sedangkan RPPT adalah kelompok bakteri menguntungkan yang agresif mengolonisasi rizosfer.…”

Section: Pendahuluanunclassified

“…Bakteri endofit adalah bakteri yang mengolonisasi jaringan tanaman sehat tanpa menyebabkan penyakit pada inangnya, sedangkan RPPT adalah kelompok bakteri menguntungkan yang agresif mengolonisasi rizosfer. Bakteri Pseudomonas fluorescens RH4003 (Nawangsih et al 2007) dan Bacillus subtilis AB89 adalah RPPT yang telah berhasil diisolasi dari perakaran tomat. Aplikasi di lapangan mampu menekan perkembangan penyakit layu bakteri.…”

Section: Pendahuluanunclassified

Interaksi antara Bakteri Endofit dan Bakteri Perakaran Pemacu Pertumbuhan Tanaman dalam Menekan Penyakit Layu Bakteri pada Tomat

Nawangsih

Wardani

2014

jfi

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ABSTRAKSalah satu faktor yang mempengaruhi produksi tomat ialah adanya penyakit layu bakteri yang disebabkan oleh Ralstonia solanacearum. Salah satu alternatif pengendalian yang ramah lingkungan untuk mengelola penyakit ini ialah dengan memanfaatkan agens biokontrol. Aplikasi bakteri endofit Staphylococcus epidermidis BC4 dan rizobacteria pemacu pertumbuhan tanaman (RPPT) (Pseudomonas fluorescens RH4003 dan Bacillus subtilis AB89) diharapkan menjadi alternatif tersebut. Penelitian ini bertujuan mengevaluasi interaksi antara bakteri endofit dan RPPT dalam menekan perkembangan penyakit layu bakteri dan pemacuan pertumbuhan tomat. Perlakuan bakteri yang dapat memacu pertambahan tinggi dan bobot kering tanaman ialah kombinasi antara 75% S. epidermidis BC4 dengan 25% P. fluorescens RH4003. Perlakuan yang dapat menekan insidensi penyakit paling tinggi ialah perlakuan P. fluorescens RH4003 yang diaplikasikan secara tunggal. Indeks penekanan penyakit oleh S. epidermidis BC4 dan P. fluorescens RH4003 secara tunggal berturut-turut mencapai 41.18 dan 45.88%. Interaksi antara bakteri endofit dan kedua bakteri perakaran pemacu pertumbuhan tanaman bersifat antagonis. Kata kunci: Bacillus subtilis, bakteri endofit, PGPR, Pseudomonas fluorescens, Staphylococcus epidermidis ABSTRACTOne of the factors affected production of tomato is the incidence of bacterial wilt disease caused by Ralstonia solanacearum. An alternative control to manage the disease which is environmentally friendly is the use of biocontrol agents. Application of endophytic bacteria (Staphylococcus epidermidis BC4) and plant growth promoting rhizobacteria (Pseudomonas fluorescens RH4003 and Bacillus subtilis AB89) as biocontrol of tomato bacterial wilt was expected to be an alternative method. The objective of this study is to evaluate the interaction among endophytic bacteria and PGPR to suppress the development of bacterial wilt disease (R. solanacearum) and promoting the growth of tomato. Bacterial treatment which caused the highest suppression on disease incidence was single application of P. fluorescens RH4003. Disease index caused by S. epidermidis BC4 and P. fluorescens RH4003 applied individually was up to 41.18 dan 45.88%, respectively. Interaction between the endophytic bacteria and both of the PGPRs were antagonistic.

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Selection and Characterization of Endophytic Bacteria as Biocontrol Agents of Tomato Bacterial Wilt Disease

Cited by 42 publications

References 12 publications

Endophytic Streptomyces spp. as Biocontrol Agents of Rice Bacterial Leaf Blight Pathogen (Xanthomonas oryzae pv. oryzae)

Endophytic Streptomyces spp. as Biocontrol Agents of Rice Bacterial Leaf Blight Pathogen (Xanthomonas oryzae pv. oryzae)

Biocontrol of Fusarium Wilt and Growth Promotion of Tomato Plants Using Endophytic Bacteria Isolated from Solanum elaeagnifolium Stems

Interaksi antara Bakteri Endofit dan Bakteri Perakaran Pemacu Pertumbuhan Tanaman dalam Menekan Penyakit Layu Bakteri pada Tomat

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