Selection and Characterization of the Virulent Fusarium oxysporum f. sp. lycopersici Isolate Inciting Vascular Wilt of Tomato

Sidharthan, V. Kavi; Aggarwal, Rashmi; Surenthiran, N.; Pothiraj, Govindan; Kowsalya, P.; Shanmugam, V.

doi:10.20546/ijcmas.2018.702.212

Cited by 14 publications

(11 citation statements)

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“…Recently, Ren et al (2018) also reported the EC 50 value of AITC as 0.95 mg L −1 against F. oxysporum in a fumigation bioassay. It is noteworthy that the TOFU-IHBT isolate used in this study was one of the most virulent strains of Fol (Sidharthan et al, 2018). In this regard, MEO exhibited promising potential against this hard-tomanage strain of Fol, as the MEO test emulsion performed better than commercial formulation.…”

Section: Discussionmentioning

confidence: 82%

“…A highly virulent Fol strain, TOFU-IHBT, available at the laboratory collections of the Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, was used as the test pathogenic fungus (Sidharthan et al, 2018). The fungal culture was activated by sub-culturing on a PDA medium and incubation at 27 ± 1 • C.…”

Section: Antifungal Bioassaymentioning

confidence: 99%

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Deciphering the Behavioral Response of Meloidogyne incognita and Fusarium oxysporum Toward Mustard Essential Oil

et al. 2021

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Environmental concerns related to synthetic pesticides and the emphasis on the adoption of an integrated pest management concept as a cardinal principle have strengthened the focus of global research and development on botanical pesticides. A scientific understanding of the mode of action of biomolecules over a range of pests is key to the successful development of biopesticides. The present investigation focuses on the in silico protein-ligand interactions of allyl isothiocyanate (AITC), a major constituent of black mustard (Brassica nigra) essential oil (MEO) against two pests, namely, Meloidogyne incognita (Mi) and Fusarium oxysporum f. sp. lycopersici (Fol), that cause severe yield losses in agricultural crops, especially in vegetables. The in vitro bioassay results of MEO against Mi exhibited an exposure time dependent on the lethal concentration causing 50% mortality (LC50) values of 47.7, 30.3, and 20.4 μg ml−1 at 24, 48, and 72 h of exposure, respectively. The study revealed short-term nematostatic activity at lower concentrations, with nematicidal activity at higher concentrations upon prolonged exposure. Black mustard essential oil displayed excellent in vitro Fol mycelial growth inhibition, with an effective concentration to cause 50% inhibition (EC50) value of 6.42 μg ml−1. In order to decipher the mechanism of action of MEO, its major component, AITC (87.6%), which was identified by gas chromatography–mass spectrometry (GC-MS), was subjected to in silico docking and simulation studies against seven and eight putative target proteins of Mi and Fol, respectively. Allyl isothiocyanate exhibited the highest binding affinity with the binding sites of acetyl cholinesterase (AChE), followed by odorant response gene-1 (ODR1) and neuropeptide G-protein coupled receptor (nGPCR) in Mi, suggesting the possible suppression of neurotransmission and chemosensing functions. Among the target proteins of Fol, AITC was the most effective protein in blocking chitin synthase (CS), followed by 2,3-dihydroxy benzoic acid decarboxylase (6m53) and trypsinase (1try), thus inferring these as the principal molecular targets of fungal growth. Taken together, the study establishes the potential of MEO as a novel biopesticide lead, which will be utilized further to manage the Mi–Fol disease complex.

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Section: Discussionmentioning

confidence: 82%

Section: Antifungal Bioassaymentioning

confidence: 99%

Deciphering the Behavioral Response of Meloidogyne incognita and Fusarium oxysporum Toward Mustard Essential Oil

et al. 2021

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“…Potato dextrose agar (PDA) medium was used to determine colony morphology, growth rates and pigmentation. For the production of chlamydospores, macro-and microconidia, the medium CLA (carnation leaf agar) was used (43,47). For molecular identification, total DNA from the fungus recovery from agar medium was obtained as template in polymerase chain reaction (PCR).…”

Section: Fungus Isolation and Molecular Identificationmentioning

confidence: 99%

Anti-infective properties of medicinal plants from the Baja California peninsula, Mexico for the treatment of Fusarium oxysporum f. sp. basilici in organic sweet basil (Ocimum basilicum)

et al. 2021

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Certified-organic farming systems in Baja California Peninsula and Northwest Mexico are nationally and globally recognized, especially due to the production of vegetables and aromatic herbs under protected agriculture systems. Based on the background of some species of the flora of Baja California Sur (BCS) to inhibit a diversity of microorganisms, the effect of 22 medicinal plants of the region was explored to know the in vitro activity against the fungus Fusarium oxysporum f. sp. basilici isolated from basil (Ocimum basilicum L.). The plants processed as crude ethanolic and aqueous extracts were analyzed in duplicate (three replicates) evaluating the inhibition of mycelial growth and spore germination. In mycelial inhibition test, all plants extracts (1000 mg L-1) showed an effectiveness of 11 to 40% to inhibit F. oxysporum. The most effective plant extracts according to 50% effective inhibition dose (ED50), were Larrea tridentata, Hymenoclea monogyra and Lippia palmeri with an ED50 of 220, 303 and 3000 mg L-1, respectively. Tukey’s PostHoc tests indicated that H. monogyra and L. tridentata are ten times (ED50 <300 mg L-1) more effective than L. palmeri (ED50 3000 mg L-1). In addition, the dose-response trend analyzes according to the logarithmic-logistic model (drc packages), showed the maximum slope values between 100 and 1000 mg L-1. In the spore germination inhibition tests, most ethanolic extracts (5000 mg L-1) showed an effectiveness between 21 and 80%. The results of this study demonstrated that the inhibitory potential of these plants used in BCS traditional medicine are a viable alternative for the control of F. oxysporum f. sp. basilici in organic basil production systems. Highlights 22 medicinal native plants from the Baja California peninsula can effectively inhibit (in vitro) oxysporum f. sp. basilica. The most effective species were Larrea tridentata, Hymenoclea monogyra and Lippia palmeri with a mycelial growth inhibition (ED50) of 220, 300 and 3000 mg L-1, respectively. In vitro effectiveness of Fusarium-growth inhibition of medicinal plants of BCS were in ED50 ranges from 220 to 3000 mg L-1. Creosote bush (L. tridentata) extract at a dose of 5000 mg L-1 can inhibit the fungal sporulation by up 80%. The ethanol-aqueous extract of the medicinal plants of BCS have the potential to be used for long-term control of the fusariosis disease in basil.

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“…Among the fungal species responsible for the deterioration of tomato fruits are those belonging to the genus Fusarium responsible for Fusariosis (Mugao, 2010;Abu et al, 2013;Ayele et al, 2021). Fusarium species belonging to this genus are the most frequent pathogens on tomato fruits, causing leaf wilt and numerous rots of the different organs of tomato (Kavi et al, 2018). These pathogens cause brown to black discoloration of the fruit.…”

Section: Introductionmentioning

confidence: 99%

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2021

Int. J. Curr. Res. Biosci. Plant Biol.

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Selection and Characterization of the Virulent Fusarium oxysporum f. sp. lycopersici Isolate Inciting Vascular Wilt of Tomato

Cited by 14 publications

References 0 publications

Deciphering the Behavioral Response of Meloidogyne incognita and Fusarium oxysporum Toward Mustard Essential Oil

Deciphering the Behavioral Response of Meloidogyne incognita and Fusarium oxysporum Toward Mustard Essential Oil

Anti-infective properties of medicinal plants from the Baja California peninsula, Mexico for the treatment of Fusarium oxysporum f. sp. basilici in organic sweet basil (Ocimum basilicum)

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