Selection and identification of a novel ssDNA aptamer targeting human skeletal muscle

Sun, Shuming; Liu, Han; Yan, Hu; Wang, Yanpeng; Zhao, Mingri; Yuan, Y.; Han, Yafei; Jing, Yingying; Cui, Jinghua; Ren, Xiaoxiang; Chen, Xiao; Su, Jiacan

doi:10.1016/j.bioactmat.2022.05.016

Cited by 33 publications

(15 citation statements)

References 68 publications

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“…It is further suggested that DEX has a highly selective excitatory effect on α 2 adrenalin receptors in the locus coeruleus of the brain and can effectively inhibit the discharge function of neurons, thus exerting analgesic and sedative effects. Therefore, increasing the amount of DEX during surgery can significantly prolong the anesthesia time and effect [ 20 , 21 ]. However, at the same time, high-dose DEX over inhibited neurons in the brain, resulting in a corresponding increase in subsequent eye opening time.…”

Section: Related Workmentioning

confidence: 99%

[Retracted] Effects of Dexmedetomidine on Hemodynamics and Anesthesia Effect of Different Doses of General Anesthesia in Patients Undergoing Hepatobiliary Surgery

Xue

et al. 2022

Contrast Media & Molecular Imaging

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In order to provide corresponding anesthesia methods of hepatobiliary diseases and obtain better clinical effect, the hemodynamics and clinical efficacy are evaluated by using different doses of dexamethasone (DEX) during the operation. 97 patients undergoing general anesthesia for hepatobiliary surgery in our hospital from April 2020 to August 2021 are analyzed retrospectively. All patients are divided into G1 (n = 24), G2 (n = 24), G3 (n = 24), and control group (n = 25) according to the random number table method. The G1, G2, and G3 groups are injected with DEX 1.0 ug/kg, DEX 0.8 ug/kg, and DEX 0.5 ug/kg by intravenous infusion pump before induction of general anesthesia, and the control group is injected with 0.9%Nacl solution 10 mL. Intraoperative anesthesia time, postoperative eye opening time, and extubation time of all groups are observed, and the incidences of postoperative adverse reactions are compared. The experimental results show that during general anesthesia in the liver and gallbladder surgery, the patients with high dose of DEX can better maintain the intraoperative hemodynamic parameters, and effectively restrain the postoperative stress reaction.

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Section: Related Workmentioning

confidence: 99%

[Retracted] Effects of Dexmedetomidine on Hemodynamics and Anesthesia Effect of Different Doses of General Anesthesia in Patients Undergoing Hepatobiliary Surgery

Xue

et al. 2022

Contrast Media & Molecular Imaging

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“…Western blot is used to detect PD-1 and PD-L1 proteins from T1 to T2 [15][16][17]. e liver cancer tissue is thoroughly ground, 200 μl of lysis buffer is added, and an ice bath is performed for 30 min.…”

Section: Detection Of Pd-1 and Pd-l1 Proteins By Wbmentioning

confidence: 99%

[Retracted] Analysis of the Relationship between Scleritis and T Cell Activation in Patients with Hepatocellular Carcinoma Treated with PD‐1 Carrelizumab

Yuan

Pan

Zou

et al. 2022

Contrast Media & Molecular Imaging

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In order to explore the function of inhibiting the immune effect, the relationship between programmed death receptor 1 (PD-1) carrelizumab in the treatment of hepatocellular carcinoma-induced scleritis and T cell activation is investigated. A total of 120 patients with primary liver cancer treated in the department of oncology of our hospital from July 2020 to January 2022 are selected and treated with carrelizumab. According to the occurrence of PD-1 carrelizumab treatment, the patients are divided into the scleritis group and nonscleritis group. The levels of T cells, PD-1, PD-L1 proteins, and serum inflammatory factors at different time points are compared. The experimental results show that the occurrence of scleritis after liver cancer treatment with PD-1 carrelizumab is closely associated with Treg cells, the percentage of Th17 cells, the expression of PD-1, PD-L1 proteins, and inflammatory factors. It is clearly evident that PD-1 carrelizumab can increase the risk of scleritis by affecting T cell activation.

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“…A group of researchers have used the human skeletal-muscle-specific ssDNA aptamer (HSM01) and found that it preferentially interacted with human skeletal muscle cells in vitro. The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells [ 67 ]. We also performed the additional experiments to evaluate the scaffolds effect on the human EA.hy926 cells.…”

Section: Discussionmentioning

confidence: 99%

Engineered 3D-Printed Polyvinyl Alcohol Scaffolds Incorporating β-Tricalcium Phosphate and Icariin Induce Bone Regeneration in Rat Skull Defect Model

Sun

Dai

et al. 2022

Molecules

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The skull defects are challenging to self-heal, and autologous bone graft repair has numerous drawbacks. The scaffolds for the rapid and effective repair of skull defects have become an important research topic. In this study, polyvinyl alcohol (PVA)/β-tricalcium phosphate(β-TCP) composite scaffolds containing icariin (ICA) were prepared through direct-ink three-dimensional (3D) printing technology. β-TCP in the composite scaffold had osteoconductive capability, and the ICA molecule had osteoinductive capacity. The β-TCP and ICA components in the composite scaffold can enhance the capability to repair skull defects. We show that ICA exhibited a slow-release behaviour within 80 days. This behaviour helped the scaffold to continuously stimulate the formation of new bone. The results of in vitro cell compatibility experiments showed that the addition of ICA molecules contributed to the adhesion and proliferation of MC-3T3-E1 cells. The level of alkaline phosphatase secretion demonstrated that the slow release of ICA can promote the osteogenic differentiation of MC-3T3-E1 cells. The introduction of ICA molecules accelerated the in situ bone regeneration in in vivo. It is concluded that the 3D-printed PVA scaffold with β-TCP and ICA has a wide range of potential applications in the field of skull defect treatment.

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Selection and identification of a novel ssDNA aptamer targeting human skeletal muscle

Cited by 33 publications

References 68 publications

[Retracted] Effects of Dexmedetomidine on Hemodynamics and Anesthesia Effect of Different Doses of General Anesthesia in Patients Undergoing Hepatobiliary Surgery

[Retracted] Effects of Dexmedetomidine on Hemodynamics and Anesthesia Effect of Different Doses of General Anesthesia in Patients Undergoing Hepatobiliary Surgery

[Retracted] Analysis of the Relationship between Scleritis and T Cell Activation in Patients with Hepatocellular Carcinoma Treated with PD‐1 Carrelizumab

Engineered 3D-Printed Polyvinyl Alcohol Scaffolds Incorporating β-Tricalcium Phosphate and Icariin Induce Bone Regeneration in Rat Skull Defect Model

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