Selection and optimisation of a method for efficient metabolites extraction from microalgae

Serive, Benoît; Kaas, Raymond; Bérard, Jean‐Baptiste; Pasquet, Virginie; Picot, Laurent; Cadoret, Jean‐Paul

doi:10.1016/j.biortech.2012.07.105

Cited by 53 publications

(29 citation statements)

References 27 publications

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“…Porphyridium purpureum CCAP 1380.3 (bangiophyceae, rhodophyte) was selected as it contains three PBP (PE, PC and APC) in high amounts [2,33]. Pp is one of the most used species to purify PBP, and its exopolysaccharidic cell wall constitutes a mechanical barrier that limits pigments extraction using conventional soaking processes [34,35].…”

Section: Microalgaementioning

confidence: 99%

Microwave-Assisted Extraction of Phycobiliproteins from Porphyridium purpureum

Juin

Chérouvrier

Thiéry

et al. 2014

Appl Biochem Biotechnol

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In the present study, microwave-assisted extraction was first employed to extract the phycobiliproteins of Porphyridium purpureum (Pp). Freeze-dried Pp cells were subjected to microwave-assisted extraction (MAE) to extract phycoerythin (PE), phycocyanin (PC), and allophycocyanin (APC). MAE combined reproducibility and high extraction yields and allowed a 180- to 1,080-fold reduction of the extraction time compared to a conventional soaking process. The maximal PE extraction yield was obtained after 10-s MAE at 40 °C, and PE was thermally damaged at temperatures higher than 40 °C. In contrast, a flash irradiation for 10 s at 100 °C was the best process to efficiently extract PC and APC, as it combined a high temperature necessary to extract them from the thylakoid membrane to a short exposure to thermal denaturation. The extraction order of the three phycobiliproteins was coherent with the structure of Pp phycobilisomes. Moreover, the absorption and fluorescence properties of MAE extracted phycobiliproteins were stable for several months after the microwave treatment. Scanning electron microscopy indicated that MAE at 100 °C induced major changes in the Pp cell morphology, including fusion of the exopolysaccharidic cell walls and cytoplasmic membranes of adjacent cells. As a conclusion, MAE is a fast and high yield process efficient to extract and pre-purify phycobiliproteins, even from microalgae containing a thick exopolysaccharidic cell wall.

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Section: Microalgaementioning

confidence: 99%

Microwave-Assisted Extraction of Phycobiliproteins from Porphyridium purpureum

Juin

Chérouvrier

Thiéry

et al. 2014

Appl Biochem Biotechnol

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“…can be utilized for pigment and lipid extraction and unexploited biomass can be used for the production of bio-hydrogen through dark fermentation. Biomethane can be produced using residues of biomass of feedstock microalgae after lipids extraction (Serive et al 2012 ).…”

Section: Biorefi Nery Concept For Biofuels and High Value Productsmentioning

confidence: 99%

Microalgal Biomass as a Source of Renewable Energy

Raza

Husssain

Shahid

et al. 2014

Biomass and Bioenergy

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“…Various extraction techniques can be used to obtain sufficient amount of crude extract to complete the isolation of active molecules and achieve their structural elucidation but this information is readily found in the literature and will not be discussed here (for fungi: [69], for microalgae: [70]). In the process of natural product drug discovery, bioassay results guide the purification processes towards increasingly small fractions.…”

Section: High-throughput Screening Of Planktonic Resourcesmentioning

confidence: 99%

Bioprospecting Marine Plankton

et al. 2013

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The ocean dominates the surface of our planet and plays a major role in regulating the biosphere. For example, the microscopic photosynthetic organisms living within provide 50% of the oxygen we breathe, and much of our food and mineral resources are extracted from the ocean. In a time of ecological crisis and major changes in our society, it is essential to turn our attention towards the sea to find additional solutions for a sustainable future. Remarkably, while we are overexploiting many marine resources, particularly the fisheries, the planktonic compartment composed of zooplankton, phytoplankton, bacteria and viruses, represents 95% of marine biomass and yet the extent of its diversity remains largely unknown and underexploited. Consequently, the potential of plankton as a bioresource for humanity is largely untapped. Due to their diverse evolutionary backgrounds, planktonic organisms offer immense opportunities: new resources for medicine, cosmetics and food, renewable energy, and long-term solutions to mitigate climate change. Research programs aiming to exploit culture collections of marine micro-organisms as well as to prospect the huge resources of marine planktonic biodiversity in the oceans are now underway, and several bioactive extracts and purified compounds have already been identified. This review will survey and assess the current state-of-the-art and will propose methodologies to better exploit the potential of marine plankton for drug discovery and for dermocosmetics.

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Selection and optimisation of a method for efficient metabolites extraction from microalgae

Cited by 53 publications

References 27 publications

Microwave-Assisted Extraction of Phycobiliproteins from Porphyridium purpureum

Microwave-Assisted Extraction of Phycobiliproteins from Porphyridium purpureum

Microalgal Biomass as a Source of Renewable Energy

Bioprospecting Marine Plankton

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