Selection for improved resistance to Aeromonas hydrophila in Indian major carp Labeo rohita: Survival and innate immune responses in first generation of resistant and susceptible lines

Sahoo, P.K.; Rauta, Pradipta Ranjan; Mohanty, B.R.; Mahapatra, Kanta Das; Saha, Jatindra Nath; Rye, Morten; Ambekar, Jalindar D.

doi:10.1016/j.fsi.2011.06.014

Cited by 64 publications

(44 citation statements)

References 51 publications

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“…Mortalities occur quickly (sometimes within 30 hours after experimental challenge, [4]) and differences in the challenge infection procedure are believed to affect expression of the genetic potential to survive this disease. Even so, one generation of divergent selection based on challenge test data has been shown to result in significantly higher average rates of survival (73.3 ± 3.3% versus 16.7 ± 3.3%), blood phagocyte respiratory burst activity, serum myeloperoxidase activity and ceruloplasmin level in resistant compared to susceptible line rohu [5]. A major limitation to selective breeding is the inability to directly test highly valuable broodstock by challenging them to the disease.…”

Section: Introductionmentioning

confidence: 99%

A linkage map of transcribed single nucleotide polymorphisms in rohu (Labeo rohita) and QTL associated with resistance to Aeromonas hydrophila

Robinson

Baranski²,

Mahapatra

et al. 2014

BMC Genomics

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BackgroundProduction of carp dominates world aquaculture. More than 1.1 million tonnes of rohu carp, Labeo rohita (Hamilton), were produced in 2010. Aeromonas hydrophila is a bacterial pathogen causing aeromoniasis in rohu, and is a major problem for carp production worldwide. There is a need to better understand the genetic mechanisms affecting resistance to this disease, and to develop tools that can be used with selective breeding to improve resistance. Here we use a 6 K SNP array to genotype 21 full-sibling families of L. rohita that were experimentally challenged intra-peritoneally with a virulent strain of A. hydrophila to scan the genome for quantitative trait loci associated with disease resistance.ResultsIn all, 3193 SNPs were found to be informative and were used to create a linkage map and to scan for QTL affecting resistance to A. hydrophila. The linkage map consisted of 25 linkage groups, corresponding to the number of haploid chromosomes in L. rohita. Male and female linkage maps were similar in terms of order, coverage (1384 and 1393 cM, respectively) and average interval distances (1.32 and 1.35 cM, respectively). Forty-one percent of the SNPs were annotated with gene identity using BLAST (cut off E-score of 0.001). Twenty-one SNPs mapping to ten linkage groups showed significant associations with the traits hours of survival and dead or alive (P <0.05 after Bonferroni correction). Of the SNPs showing significant or suggestive associations with the traits, several were homologous to genes of known immune function or were in close linkage to such genes. Genes of interest included heat shock proteins (70, 60, 105 and “small heat shock proteins”), mucin (5b precursor and 2), lectin (receptor and CD22), tributyltin-binding protein, major histocompatibility loci (I and II), complement protein component c7-1, perforin 1, ubiquitin (ligase, factor e4b isoform 2 and conjugation enzyme e2 c), proteasome subunit, T-cell antigen receptor and lymphocyte specific protein tyrosine kinase.ConclusionsA panel of markers has been identified that will be validated for use with both genomic and marker-assisted selection to improve resistance of L. rohita to A. hydrophila.

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Section: Introductionmentioning

confidence: 99%

A linkage map of transcribed single nucleotide polymorphisms in rohu (Labeo rohita) and QTL associated with resistance to Aeromonas hydrophila

Robinson

Baranski²,

Mahapatra

et al. 2014

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…The global production of L. rohita was approximately 1.2 million tons in 2005, out of which nearly 1 million tons was contributed by India [2]. Bacterial infections are one of the most important causes of disease problems in Indian aquaculture [3]. Aeromonas hydrophila is the most common pathogen, and it can easily spread through accidental abrasions [4].…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeo rohita

Giri

Sen

Sukumaran

2012

Fish & Shellfish Immunology

114

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“…First 15 families with higher survival record are considered as resistant line and last ten families having the lowest survival were considered as susceptible line. Offspring from both the lines were produced and challenged with same dose of A. hydrophila [14]. The second generations of resistant and susceptible lines were generated from first generation respective lines by taking 20 males and 20 females from each line.…”

Section: Differential Expression Analysismentioning

confidence: 99%

“…Furthermore, limited sequence information was available for some immune-related genes such as complement component 3 (C3), G and C-type lysozymes, interleukin 1b (IL1b), b-2 microglobulin (b-2 M), CXCa, tumor necrosis factor a (TNFa), inducible nitric oxide synthase (iNOS), few tolllike receptors (TLRs), nucleotide binding and oligomerisation domain 2 (NOD 2) and transferrin, etc. [12][13][14][15] until the high throughput Illumina mRNA-seq carried out in our earlier study [16] from comparison of first generation selected resistant and susceptible lines of rohu raised against Aeromonas hydrophila infection. The methods used to generate these lines were described in Sahoo et al [14].…”

Section: Introductionmentioning

confidence: 99%

“…[12][13][14][15] until the high throughput Illumina mRNA-seq carried out in our earlier study [16] from comparison of first generation selected resistant and susceptible lines of rohu raised against Aeromonas hydrophila infection. The methods used to generate these lines were described in Sahoo et al [14]. Gene ontology terms broadly grouped 3,419 (7.7 %) contigs as involved in stress response, while 1,939 (4.4 %) were categorised as involved in immune system processes.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of immune-related ESTs and differential expression analysis of few important genes in lines of rohu (Labeo rohita) selected for resistance and susceptibility to Aeromonas hydrophila infection

et al. 2014

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A total of 137,629 contigs generated via de novo transcriptome assembly from resistant and susceptible lines of rohu (first generation) raised against aeromoniasis were further analyzed in terms of defence-related genes. Out of 1,939 contigs showing homology to genes involved in immune processes, 1,866 were further categorised into different functional subgroups. Comparative analysis revealed five genes for the first time in any carp species out of which apolipoprotein h, septin 4 isoform 3 and septin isoform cra_c were identified for the first time in fish. Differential expression analysis of ten genes viz., heat shock proteins (Hsps) (Hsp30, Hsp70 and Hsp90), serum lectin isoform 1 (SLI1), linker histone H1M (LHH1M), NAD(P)H quinone 1 (NQO1), zona pellucida 2 (ZP2) and three unknown genes that were highly up-expressed in first generation resistant line fish from mRNA-seq coverage data, was carried out using susceptible and resistant individuals of the second generation selected populations in eight different tissues viz. liver, kidney, intestine, gill, brain, spleen, skin and muscle using qPCR. Significant up-regulation in Hsp90, NQO1, C_116914 and C_22454 in specific tissues of resistant line and variable expression in Hsp30 and LHH1M genes in different tissues of both lines were noticed. The expression of Hsp70 was lower in many tissues of the resistant line than in susceptible line rohu. The expression of ZP2, SLI1 and C_94589 genes was not significantly different in terms of fold difference between the two lines. Differentially expressed genes need further characterisation to explore their role in resistance to Aeromonas hydrophila infection in rohu.

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Selection for improved resistance to Aeromonas hydrophila in Indian major carp Labeo rohita: Survival and innate immune responses in first generation of resistant and susceptible lines

Cited by 64 publications

References 51 publications

A linkage map of transcribed single nucleotide polymorphisms in rohu (Labeo rohita) and QTL associated with resistance to Aeromonas hydrophila

A linkage map of transcribed single nucleotide polymorphisms in rohu (Labeo rohita) and QTL associated with resistance to Aeromonas hydrophila

Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeo rohita

Analysis of immune-related ESTs and differential expression analysis of few important genes in lines of rohu (Labeo rohita) selected for resistance and susceptibility to Aeromonas hydrophila infection

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