Selection of a Morphological Variant ofAutographa californicaNuclear Polyhedrosis Virus with Increased Virulence Following Serial Passage inPlutella xylostella

Kolodny-Hirsch, Douglas M.; Beek, N.A.M. van

doi:10.1006/jipa.1997.4659

Cited by 33 publications

(29 citation statements)

References 26 publications

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“…Serial passage of NPV populations in alternate insect species generally results in marked increases in pathogenicity against the alternate hosts (Martignoni & Iwai ; Weitzman et al . ; Kolodny‐Hirsch & van Beek ) and is accompanied by an altered relative abundance of individual variants (Hitchman et al . ).…”

Section: Baculovirus Diversity and Evolutionmentioning

confidence: 99%

Baculoviruses: diversity, evolution and manipulation of insects

Ikeda

Hamajima

Kobayashi

2014

Entomological Science

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Baculoviruses, members of the family Baculoviridae, are large, enveloped viruses that contain a doublestranded circular DNA genome of 80-180 kbp, encoding 90-180 putative proteins. These viruses are exclusively pathogenic for arthropods, particularly insects, and have been developed, or are being developed, as environmentally sound pesticides and eukaryotic vectors for foreign protein expression, surface display, gene delivery for gene therapy, vaccine production and drug screening. The baculoviruses contain a set of approximately 30 core genes that are conserved among all baculovirus genomes sequenced to date. Individual baculoviruses also contain a number of lineage-or species-specific genes that have greatly impacted the diversification and evolution of baculoviruses. In this review, we first describe the general properties and biology of baculoviruses and then focus on the baculovirus genes and mechanisms involved in the replication, spread and survival of baculoviruses within the context of their diversity, evolution and insect manipulation.

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Section: Baculovirus Diversity and Evolutionmentioning

confidence: 99%

Baculoviruses: diversity, evolution and manipulation of insects

Ikeda

Hamajima

Kobayashi

2014

Entomological Science

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“…Success in adaptation to homologous or heterologous hosts by serial passages has been reported for baculoviruses (21,26). The approach developed in this study was to survey existing isolates for their efficacy against the resistant codling moth and then to examine the possible adaptation of the most pathogenic candidate to the resistant host by serial passages through a laboratory colony of C. pomonella originating from field-collected resistant insects.…”

mentioning

confidence: 99%

Cydia pomonella granulovirus Genotypes Overcome Virus Resistance in the Codling Moth and Improve Virus Efficiency by Selection against Resistant Hosts

Berling¹,

Blachère-López²,

Soubabère

et al. 2009

Appl Environ Microbiol

109

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Cydia pomonella granulovirus (CpGV) has been used for 15 years as a bioinsecticide in codling moth (Cydia pomonella) control. In 2004, some insect populations with low susceptibility to the virus were detected for the first time in southeast France. RGV, a laboratory colony of codling moths resistant to the CpGV-M isolate used in the field, was established with collection of resistant insects in the field followed by an introgression of the resistant trait into a susceptible colony (Sv). The resistance level (based on the 50% lethal concentrations [LC 50 s]) of the RGV colony to the CpGV-M isolate, the active ingredient in all commercial virus formulations in Europe, appeared to be over 60,000-fold compared to the Sv colony. The efficiency of CpGV isolates from various other regions was tested on RGV. Among them, two isolates (I12 and NPP-R1) presented an increased pathogenicity on RGV. I12 had already been identified as effective against a resistant C. pomonella colony in Germany and was observed to partially overcome the resistance in the RGV colony. The recently identified isolate NPP-R1 showed an even higher pathogenicity on RGV than other isolates, with an LC 50 of 166 occlusion bodies (OBs)/l, compared to 1.36 ؋ 10 6 OBs/l for CpGV-M. Genetic characterization showed that NPP-R1 is a mixture of at least two genotypes, one of which is similar to CpGV-M. The 2016-r4 isolate obtained from four successive passages of NPP-R1 in RGV larvae had a sharply reduced proportion of the CpGV-M-like genotype and an increased pathogenicity against insects from the RGV colony.

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“…Studies on baculoviruses have demonstrated that viral isolate pathogenicity varies depending on propagation host species due to morphological changes in the virus such as the number of nucleocapsids per virion or virions per OB (Tompkins et al, 1981) and changes in viral identity (Kolodny-Hirsch and van Beek, 1997). Examples show that the number of passages required for a change in pathogenicity vary from only one passage (Stairs et al, 1981) to several passages (Tompkins et al, 1981(Tompkins et al, , 1988Kolodny-Hirsch and van Beek, 1997). AdhoEPV-Mi behavior differed from that of AdhoEPV-It and AdhoEPV-Ts in H. magnanima; AdhoEPV-Mi had different replication characteristics in H. magnanima.…”

Section: Discussionmentioning

confidence: 99%