Selection of antagonistically active strains of lactic acid bacteria from milk of various animal species

Aitzhanova, Aida; Oleinikova, E. A.; Saubenova, M. G.; Daugalieva, S. T.; Berzhanova, Ramza Zh.

doi:10.26577//eb.2020.v83.i2.08

Cited by 3 publications

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“…The scientifically established phenomenon of the protective function of the lactobacilli's biofilm is promising for further use in the medical, pharmaceutical, food and agricultural industries, which is a priority for Kazakhstan (Aitzhanova et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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2023

JJBS

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Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscript that meets the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately one to two months after acceptance.

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Section: Introductionmentioning

confidence: 99%

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2023

JJBS

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Section: Introductionmentioning

confidence: 99%

The ability of Lactobacillus helveticus -13 (Lh-13) Isolate Isolated from Lactic Acid Products to form A biofilm by Applying Modern Microscopy Methods

2023

JJBS

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This article presents the study of the morpho-cultural properties and biofilm formation of lactobacilli isolated from lactic acid products (irimchik, suzbe, cheese, ashygan kozhe) produced in different districts of the Karaganda region. The species of the isolated lactic acid bacillus isolates were identified by using a MALDI-TOF mass spectrometer. The evaluation of the resistance of the isolates to various stress factors, such as the ability to survive at low acidic pH values and in the presence of bile, made it possible to identify some promising isolates -Lactobacillus helveticus -13 (Lh-13) and Lactiplantibacillus plantarum -5 (Lpl-5), for studying antimicrobial and biofilm-forming activity. The antimicrobial activity of Lh-13, Lpl-5 was closely studied. They have a certain level of antibacterial and fungicidal activities, which indicates their pronounced inhibition against indicator microorganisms. Using a laboratory robot, TecanEVolizer100 (Tecan), the optical density of Lh-13, Lpl-5 was studied in the wells of polystyrene plates, reflecting the intensity of biofilm formation along their surface. Using laser capture microdissection, the images of cell morphology in biofilm of Lh-13 isolates included in the matrix were obtained. The atomic force microscopy made it possible to study the two and three-dimensional morphological images of biofilm-forming isolates of Lh-13.

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“…The nutritional peculiarities of the population of Kazakhstan have a long history of traditional lactic acid products from milk of cows, mares, camels, and goats and are based on milk fermentation [1]. To date, the study of probiotic strains with highly active biological properties isolated from local Kazakh lactic acid products with a long history is of high priority [2].…”

Section: Introductionmentioning

confidence: 99%

Screening of Antimicrobial and Adhesive Activity of Lactobacilli Isolated from the National Food Products from Different Districts of the Karaganda Region (Kazakhstan)

Amirkhanova

Ахметова

Kozhakhmetov

et al. 2021

Open Access Maced J Med Sci

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BACKGROUND: It is a national priority to look for new probiotic bacteria with highly active biological properties to create a new generation of probiotics, ferments, therapeutic, and prophylactic fermented milk products, taking into account ethnocultural and regional characteristics. AIM: The aim of the study is to assess probiotic properties of strains of lactobacilli (antimicrobial and adhesive), which are isolated from national lactic acid products from different districts of the Karaganda region (Kazakhstan). MATERIALS AND METHODS: There were modern microbiological methods applied during the experiment. To determine the morpho-cultural properties, the following methods were used: Gram staining, a catalase test, serial dilutions. The Matrix Supported Laser Desorption/Ionization Flight Time Mass Spectrometry was used for identification, and the deferred-antagonism method was used to determine the antimicrobial activity. The buccal epithelial cells were used for the cell object as a test system to determine the adhesive activity. RESULTS: In this experiment, 26 lactobacillus isolates were isolated from 68 samples of national lactic acid products produced in a traditional homemade way in different districts of the Karaganda region (Kazakhstan). As a result of the studies carried out on the cultural and morphological characteristics and identification by the mass spectrometer, the following lactobacilli were obtained: Lactobacillus acidophilus (two strains), Lactobacillus delbrueckii subsp. bulgaricum (two strains), Lactobacillus rhamnosus (seven strains), Lactobacillus plantarum (two strains), Lactobacillus paracasei (11 strains), and Lactobacillus fermentum (two strains). Twenty-six isolates of lactobacilli were tested for antimicrobial activity, 13 isolates of which showed an inhibitory effect, but the degree of antagonism varied among lactobacillus isolates. In general, the inhibitory activity of lactobacillus isolates was shown against the Gram-negative indicator microorganisms Salmonella typhimurium NCTC 12023, Escherichia coli NCTC 12923. The antibacterial activity was shown against the Staphylococcus aureus NCTC 12973 indicator microorganism in nine isolates of lactobacilli. Only six isolates of lactobacilli showed antifungal activity against the test strain of Candida albicans NCPF 3179. Out of 13 isolates of lactobacilli, nine isolates of medium and high activity competed for binding to buccal epithelial cells. CONCLUSION: The obtained isolates from traditional dairy products are considered to be promising candidates and competitive isolates with some probiotic potential. This study calls for further researches to be made in this area.

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Selection of antagonistically active strains of lactic acid bacteria from milk of various animal species

Cited by 3 publications

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The ability of Lactobacillus helveticus -13 (Lh-13) Isolate Isolated from Lactic Acid Products to form A biofilm by Applying Modern Microscopy Methods

Screening of Antimicrobial and Adhesive Activity of Lactobacilli Isolated from the National Food Products from Different Districts of the Karaganda Region (Kazakhstan)

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