Selection of exopolysaccharide-producing lactic acid bacteria isolates from Inner Mongolian traditional yoghurt

Screening for compounds that can neutralize the toxicity of tetrodotoxin (TTX) or reduce its negative effects is necessary. Our study tested the TTX detoxification capacity of exopolysaccharide (EPS) extracted from lactic acid bacteria. EPS of Leuconostoc mesenteroides N3 isolated from the Vung Tau sea (Vietnam), Lactobacillus plantarum PN05, and Lactobacillus rhamnosus PN04 were used in the study. To more completely evaluate the importance of EPS in detoxification, EPS samples of Leuconostoc mesenteroides N3, Lactobacillus plantarum PN05 and Lactobacillus rhamnosus PN04 were also tested. The majority of EPS of these bacteria contained glucose; this was observed using thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis. As observed with FTIR analysis, only EPS of Lactobacillus plantarum PN05 contained methyl groups. The results indicated that detoxification of TTX in mice could be obtained at an optimal dose of 248 µg EPS from Leuconostoc mesenteroides incubated with 54 µg cuprous oxide for 40 min or 148 µg EPS Lactobacillus rhamnosus incubated with 55 µg cuprous oxide for 40 min, while EPS from Lactobacillus plantarum showed TTX detoxification capacity without cuprous oxide combination. Consequently, EPS from Lactobacillus plantarum PN05 can be used in TTX prevention. This is the first report on the importance of lactic acid bacteria in TTX detoxification.

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“…The EPS concentration was determined by the phenol-sulphuric acid method [23]. The absorbance was measured at 490 nm.…”

Section: Methodsmentioning

confidence: 99%

Detection of the Potential Inactivation of Tetrodotoxin by Lactic Acid Bacterial Exopolysaccharide

Dat

Canh

et al. 2018

Toxins

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“…(Figure 1). The detection of EPS production by lactic acid producing bacteria is frequently accomplished through the screening of mucosal colonies, as reported in literature [21]. Prior studies have effectively detected bacteria that produce extracellular polymeric substances (EPS) and identified the presence of biofilm and its associated colonies of bacteria.…”

Section: Detection Of Weissella Cibaria For the Production Of Dextranmentioning

confidence: 99%

Production of Dextran from Domestic Weissella cibaria Isolated

Suheil,

Ahmaed

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Dextran is an exopolysaccharide (EPS) of bacterial origin that is readily available in the commercial market. that is used in various industrial applications as an adjuvant, emulsifier, carrier, and stabilizer in the food and biomedical industries. Industrial production of dextran is carried out by fermentation in sucrose-rich media. Studies to optimize dextran production have shown that dextran yield varies depending on specific production conditions. This study aimed to generate dextrans Lactobacillus species were obtained from the vaginal and stool samples of healthy infants. The mucosal technique was employed to determine the biosynthesis of dextran from various isolates of Lactobacillus spp. colony assay and confirmed by isopropanol precipitation. The effects of various parameters such as sucrose concentration, nitrogen concentration, temperature, incubation time, pH, and inoculum size were studied to optimize the maximum dextran yield. In the present study, Weissella cibaria may produce dextran. Optimal conditions for dextran production are a 48-hour incubation at The experimental conditions involved maintaining a temperature of 37°C, with a sugar concentration of 10%, and a yeast extract concentration of 0.5%. The inoculum volume was set at 4%, and the pH level was maintained at 6.5. The experiment was conducted under aerobic conditions. The obtained value for dextran dry weight was 520 mg/100 ml.

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“…Ltd, China; molecular weight cut-off, 8-14kDa) for 2 days. The EPS yield was determined by the phenol-sulphuric acid method according to Dubois et al (1956);Chun-lei et al, (2014).…”

Section: Extraction and Quantification Of Exopolysaccharidementioning

confidence: 99%

“…Following inoculation, the 10ml inocula were completely transferred into the 90ml broth; then incubated at 40 o C for 36h. After which EPS was extracted and quantified as earlier described, according to the method of Chun-lei et al, (2014).…”

Section: Umyu Journal Of Microbiology Research Wwwujmrumyuedung E-iss...mentioning

confidence: 99%

Screening For Potential Exopolysaccharide Producers From Lactobacillus spp Isolated From Locally Fermented Milk (Nono)

Kabir¹,

Shamsuddeen²,

Magashi³

2022

UJMR

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Exopolysaccharides (EPS) are exogenous bacterial sugar polymers with many applications in dairy, pharmaceutical and cosmetic industries, using it as thickeners, stabilizers, and gelling agents. The study aimed to screen for potential exopolysaccharide producers from Lactobacillus spp. isolated from locally fermented milk (nono). Twenty-five nono Samples were collected from Wambai market, Kano. Lactic acid bacteria were isolated using de Man Ragosa and Sharpe Agar. Isolates were identified by API 50 CHL kit and web and screened for EPS production in which the EPS was extracted and quantified using the phenol-sulphuric method. Next, the influence of carbon source (Glucose, Sucrose and Lactose) and concentrations on EPS were evaluated on some of the isolated strains. The functional groups of the EPS were confirmed using FTIR. The isolated Lactobacillus spp. were all Gram positive, catalase and oxidase negative, API identification yielded; Lactobacillus acidophilus 1, Lb. brevis 1, Lb. fermentum, Lb. paracasei ssp paracasei, Lb. acidophilus 3. Ten isolates yielded EPS in the range of 248.33mg/l - 07.83mg/l. The FTIR analysis of extracted EPS produced peaks around 3,300–881cm-1. Hence the study has brought to light the presence of potential EPS producing LAB in nono, which could be further exploited to harness their potential. Keywords: Exopolysaccharide, Lactic acid Bacteria, Lactobacillus spp, Kano

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Selection of exopolysaccharide-producing lactic acid bacteria isolates from Inner Mongolian traditional yoghurt

Cited by 8 publications

References 6 publications

Detection of the Potential Inactivation of Tetrodotoxin by Lactic Acid Bacterial Exopolysaccharide

Detection of the Potential Inactivation of Tetrodotoxin by Lactic Acid Bacterial Exopolysaccharide

Production of Dextran from Domestic Weissella cibaria Isolated

Screening For Potential Exopolysaccharide Producers From Lactobacillus spp Isolated From Locally Fermented Milk (Nono)

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