1964
DOI: 10.1038/202928a0
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Selection of Media for Isolation of Streptomycetes

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Cited by 784 publications
(338 citation statements)
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“…The suspension was diluted with 45 ml saline containing 1.5% phenol (w/v) and shaken with glass beads for 30 minutes at 30°C. After filtration through sterile glass wool and dilution with saline (10 Ϫ1 , 10 Ϫ2 and 10 Ϫ3 , respectively), 0.1 ml aliquots were spread onto starch -casein agar [9], containing cycloheximide (50 m g/ml), nystatin (50 m g/ml), nalidixic acid (20 mg/ml) and novobiocin (20 mg/ml). The plates were incubated 14 days at 27°C, and the resulting colonies were transferred to starch -casein agar and ISP-2 agar [10].…”
Section: Microorganismmentioning
confidence: 99%
“…The suspension was diluted with 45 ml saline containing 1.5% phenol (w/v) and shaken with glass beads for 30 minutes at 30°C. After filtration through sterile glass wool and dilution with saline (10 Ϫ1 , 10 Ϫ2 and 10 Ϫ3 , respectively), 0.1 ml aliquots were spread onto starch -casein agar [9], containing cycloheximide (50 m g/ml), nystatin (50 m g/ml), nalidixic acid (20 mg/ml) and novobiocin (20 mg/ml). The plates were incubated 14 days at 27°C, and the resulting colonies were transferred to starch -casein agar and ISP-2 agar [10].…”
Section: Microorganismmentioning
confidence: 99%
“…Morphological observations were made on plates containing oatmeal agar, thin potato-carrot agar, water agar, and soil extract agar (43). The additional media used to characterize strain SK&F-AAD-216T were inorganic salts-starch agar (44), peptone-yeast extract-iron agar (44), starch-casein-nitrate agar (22), DNase Test Agar (BBL Microbiology Systems), Bennett agar (17), tyrosine agar (44), gelatin medium 19 (49), thin Pablum agar (31), and litmus milk medium (Difco Laboratories). All tests were performed at * Corresponding author.…”
Section: Methodsmentioning
confidence: 99%
“…After grinding lichen samples with mortar and pestle, a homogenate was prepared using sterile 0.85% NaCl in a 1:10 (w/v) ratio, together with a lab stomacher (BagMixer; Interscience, St Nom, France). Diluted fractions were plated on R2A agar (Carl Roth, Karlsruhe, Germany), R2A agar with 25 mg ml À 1 cycloheximide, starch casein agar (Kuester and Williams, 1964) and ISP2 agar (Shirling and Gottlieb, 1966). Bacterial colonies were randomly picked within 5 days of incubation at room temperature.…”
Section: Isolation Of Lichen-associated Bacteriamentioning
confidence: 99%